Comparative effect of Piper betle, Chlorella vulgaris and tocotrienol-rich fraction on antioxidant enzymes activity in cellular ageing of human diploid fibroblasts

Abstract

BackgroundHuman diploid fibroblasts (HDFs) undergo a limited number of cellular divisions in culture and progressively reach a state of irreversible growth arrest, a process termed cellular ageing. Even though beneficial effects of Piper betle, Chlorella vulgaris and tocotrienol-rich fraction (TRF) have been reported, ongoing studies in relation to ageing is of interest to determine possible protective effects that may reverse the effect of ageing. The aim of this study was to evaluate the effect of P. betle, C. vulgaris and TRF in preventing cellular ageing of HDFs by determining the activity of antioxidant enzymes viz.; catalase, superoxide dismutase (SOD) and glutathione peroxidase.MethodsDifferent passages of HDFs were treated with P. betle, C. vulgaris and TRF for 24 h prior to enzymes activity determination. Senescence-associated beta-galactosidase (SA β-gal) expression was assayed to validate cellular ageing.ResultsIn cellular ageing of HDFs, catalase and glutathione peroxidase activities were reduced, but SOD activity was heightened during pre-senescence. P. betle exhibited the strongest antioxidant activity by reducing SA β-gal expression, catalase activities in all age groups, and SOD activity. TRF exhibited a strong antioxidant activity by reducing SA β-gal expression, and SOD activity in senescent HDFs. C. vulgaris extract managed to reduce SOD activity in senescent HDFs.ConclusionP. betle, C. vulgaris, and TRF have the potential as anti-ageing entities which compensated the role of antioxidant enzymes in cellular ageing of HDFs.

Tocotrienol‐Rich Fraction Ameliorates Antioxidant Defense Mechanisms and Improves Replicative Senescence‐Associated Oxidative Stress in Human Myoblasts

“…SOD1 and GPX1 mRNA expression were not significantly different across the cell stages. However, SOD2 and CAT mRNA expression were upregulated in presenescent myoblasts, which was similar to Sod activity in a previous study [ 32 ]. Additionally, a decline in Cat activity was observed in presenescent myoblasts compared to young myoblasts.…”

Treatment with Modified Extracts of the Microalga Planktochlorella nurekis Attenuates the Development of Stress-Induced Senescence in Human Skin Cells

“…Data on Chlorella extract-associated anti-aging action are limited to selected models of replicative senescence, namely, human fibroblasts and myocytes at different passage numbers (young, pre-senescent, and senescent cells) [24,27,[43][44][45] and UV-induced cytotoxicity and senescence [46,47]. Hot water extract of Chlorella vulgaris lowered the levels of DNA damage in young, pre-senescent, and senescent fibroblasts in control conditions [27] and during oxidative stress [43].…”

“…Hot water extract of Chlorella vulgaris lowered the levels of DNA damage in young, pre-senescent, and senescent fibroblasts in control conditions [27] and during oxidative stress [43]. However, the antioxidant activity of hot water extract of Chlorella vulgaris was limited to pre-senescent fibroblasts as judged by the extract-mediated increase in catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activity [44]. More recently, Chlorella extract-mediated modulation of the expression of genes involved in oxidative stress response and DNA damage response and insulin/insulin-like growth factor-1 signaling, cell differentiation, and cell proliferation pathways was analyzed using young, pre-senescent, and senescent fibroblasts [24].…”

AKTIVITAS SITOTOKSIK SENYAWA GOLONGAN FENOLIK DARI EKSTRAK DAUN SIRIH (Piper betle L.)

“…Penelitian dengan subjek tumbuhan sirih sudah banyak dilakukan diantaranya untuk mempelajari potensi antioksidan ekstrak daun sirih, sifat sitotoksik ekstrak etanol daun sirih merah, dan bioaktivitas antidiabetes ekstrak etanol dan ekstrak air daun sirih (Makpol et al, 2013, Emrizal et al, 2014, dan Arambewela et al, 2005. Dari berbagai hasil penelitian daun sirih diketahui secara luas memiliki sifat-sifat antifungi, antioksidan, antiplatelet, antipiretik, antiinflamasi, antitrombotik, dan sebagai depresan (Sripradha, 2014).…”