Treatment with Modified Extracts of the Microalga Planktochlorella nurekis Attenuates the Development of Stress-Induced Senescence in Human Skin Cells

Abstract

More recently, we have proposed a safe non-vector approach to modifying the biochemical profiles of the microalga Planktochlorella nurekis and obtained twelve clones with improved content of lipids and selected pigments and B vitamins and antioxidant activity compared to unaffected cells. In the present study, the biological activity of water and ethanolic extracts of modified clones is investigated in the context of their applications in the cosmetic industry and regenerative medicine. Extract-mediated effects on cell cycle progression, proliferation, migration, mitogenic response, apoptosis induction, and oxidative and nitrosative stress promotion were analyzed in normal human fibroblasts and keratinocytes in vitro. Microalgal extracts did not promote cell proliferation and were relatively non-cytotoxic when short-term treatment was considered. Long-term stimulation with selected microalgal extracts attenuated the development of oxidative stress-induced senescence in skin cells that, at least in part, was correlated with nitric oxide signaling and increased niacin and biotin levels compared to an unmodified microalgal clone. We postulate that selected microalgal extracts of Planktochlorella nurekis can be considered to be used in skin anti-aging therapy.

Application of Green Algal Planktochlorella nurekis Biomasses to Modulate Growth of Selected Microbial Species

“…The samples were then incubated at 37 °C for 24 h with shaking (1000 RPM) and centrifuged (12,000 RPM, RT, 10 min). Supernatants were collected and stored until use at −20 °C [ 58 ].…”

Creatine and Nicotinamide Prevent Oxidant-Induced Senescence in Human Fibroblasts

“…Given this new paradigm of photocarcinogenesis involving dermal fibroblast senescence with resultant IGF-1 deficiency, therapeutics designed to prevent or treat fibroblast senesce could have use, especially in populations at high risk for skin cancer. Examples of agents currently studied for abilities to inhibit fibroblast senescence include extracts of microalga [37] or Akebia quinate fruit [38]. Additionally, Cr supplementation protects against mitochondrial mutagenesis as well as increases in matrix metalloproteinase-1 in response to UVA [39].…”

Natural and Synthetic Bioactives for Skin Health, Disease and Management

“…The authors concluded that these effects may be related to increased nitric oxide, niacin and biotin levels in cells compared to an unmodified microalgal clone. Their data suggested that selected microalgal extracts of Planktochlorella nurekis could be used in an anti-skin aging regimen [10].…”