2016
DOI: 10.1016/j.biomaterials.2016.03.034
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Comparative effect of physicomechanical and biomolecular cues on zone-specific chondrogenic differentiation of mesenchymal stem cells

Abstract: Current tissue engineering approaches to regeneration of articular cartilage rarely restore the tissue to its normal state because the generated tissue lacks the intricate zonal organization of the native cartilage. Zonal regeneration of articular cartilage is hampered by the lack of knowledge for the relation between physical, mechanical, and biomolecular cues and zone-specific chondrogenic differentiation of progenitor cells. This work investigated in 3D the effect of TGF-β1, zone-specific growth factors, op… Show more

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Cited by 44 publications
(65 citation statements)
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(81 reference statements)
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“…Fortunately, this challenge may be mitigated by understanding the relationship between the zonal microstructure and bulk tissue function. Both the arcade‐like architecture of the collagenous network and graded glycosaminoglycan content play a major role in conferring function to osteochondral tissue by dictating both the phenotype of embedded chondrocytes and mechanical properties of each zone . Without the appropriate structural and compositional cues, regenerated tissue will lack zonal properties and have comprised function; it is critical to impart zonal character in engineered osteochondral tissue.…”
Section: Discussionmentioning
confidence: 99%
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“…Fortunately, this challenge may be mitigated by understanding the relationship between the zonal microstructure and bulk tissue function. Both the arcade‐like architecture of the collagenous network and graded glycosaminoglycan content play a major role in conferring function to osteochondral tissue by dictating both the phenotype of embedded chondrocytes and mechanical properties of each zone . Without the appropriate structural and compositional cues, regenerated tissue will lack zonal properties and have comprised function; it is critical to impart zonal character in engineered osteochondral tissue.…”
Section: Discussionmentioning
confidence: 99%
“…Furthermore, the smaller elongated pore channels [48 µm, Fig. (A2)] may encourage infiltrating MSCs to adopt an elongated morphology which may help establish a superficial zone phenotype …”
Section: Discussionmentioning
confidence: 99%
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“…[7] Critically, substrates possessing bulk rigidity of circa 20-50 kPa have been previously shown to induce stem cell differentiation to cartilage and bone specific lineages in vitro. [7,17] Poly(dimethylsiloxane) (PDMS), in particular, has recently found widespread use in cell adhesion/migration assays [18] microfluidic and MEMS technologies [19] due to its favorable optical, biocompatible and mechanical properties. Indeed, PDMS substrates have been used to study the role of extracellular rigidity on cellular adhesion [20] and differentiation, [21] due to the ease by which its rigidity may be precisely controlled by simply varying the base:accelerator ratio.…”
Section: Introductionmentioning
confidence: 99%
“…Another principle used to enhance the mechanical properties of hydrogels is the use of nanofibers. These can be used to enforce a hydrogel by forming an internal random network [22] or, by aligning them, increasing the tensile strength [23] or tune the matrix compressive modulus [24].…”
Section: A C C E P T E D Accepted Manuscriptmentioning
confidence: 99%