Comparative effect of pesticides on brain acetylcholinesterase in tropical fish

Assis, Caio Rodrigo Dias; Linhares, Amanda Guedes; Oliveira, Vagne de Melo; França, Renata Cristina Penha; Carvalho, Elba Verônica Matoso Maciel de; Bezerra, Ranilson S.; Carvalho, Luiz Bezerra de

doi:10.1016/j.scitotenv.2012.09.058

Cited by 63 publications

(34 citation statements)

References 33 publications

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“…TE is a pesticide OP of the phosphorothioate group. The phosphorothioates are more lipophilic than the phosphates and are rapidly absorbed and accumulated in tissues (Assis et al, 2012). Although this pesticide is more biodegradable and less persistent than other compounds, TE has been detected globally and, due to widespread use, has created concerns for environmental health.…”

mentioning

confidence: 99%

Enzymatic Alterations and Genotoxic Effects Produced by Sublethal Concentrations of Organophosphorous Temephos in Poecilia reticulata

Pereira

Júnior

2015

Journal of Toxicology and Environmental Health, Part A

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The responses of biochemical and genetic parameters were evaluated in tissues of Poecilia reticulata exposed to sublethal and environmentally relevant concentrations of 0.005, 0.01, or 0.02 mg/L of the organophosphorous (OP) pesticide temephos (TE) for 168 h. Activities of enzymes brain acetylcholinesterase (AChE) and liver carboxylesterase (CbE) were determined. Nuclear abnormalities (NA) and micronucleus (MN) frequency in gill erythrocytes were also measured. No mortality was observed over the experimental period; however, brain AChE activities were decreased significantly in guppies in all TE treatment groups after 72 h of exposure. Hepatic CbE activities of fish were increased in all TE treatment groups at 96, 120, and 144 h of exposure. The frequencies of MN and NA in fish gill erythrocytes displayed a marked rise after 168 h of exposure to concentrations of 0.01 or 0.02 mg/L TE. Thus, determination of these parameters may be employed as potential indices of exposure to TE using this sentinel organism for monitorining.

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confidence: 99%

Enzymatic Alterations and Genotoxic Effects Produced by Sublethal Concentrations of Organophosphorous Temephos in Poecilia reticulata

Pereira

Júnior

2015

Journal of Toxicology and Environmental Health, Part A

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“…In accordance with the present results, previous studies have demonstrated that chlorpyrifos has strong inhibitory effect on AChE activity. Assis et al (2012) (Uner et al 2006). Thus, these results show that AChE is a pesticide target and can be used as a bioindicator (Oruç 2010;Pérez et al 2013).…”

Section: Discussionmentioning

confidence: 60%

Thein vitroandin vivoeffects of chlorpyrifos on acetylcholinesterase activity of rainbow trout brain

Topal

Şişecioğlu

Atamanalp

et al. 2015

Journal of Applied Animal Research

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Chlorpyrifos is widely used to control agricultural pests associated with fruit, nut and vegetable crops, despite its toxic effects and potential brain alterations in aquatic organisms. This study was carried out to determine the in vivo and in vitro effects of chlorpyrifos on rainbow trout brain acetylcholinesterase (AChE) enzyme activity. The fish were exposed to 2.25 µg/L (25% of 96 h LC 50 ), 4.5 µg/L (50% of 96 h LC 50 ) and 6.75 µg/L (75% of 96 h LC 50 ) of chlorpyrifos for 24, 48, 72 and 96 h. In vitro studies, inhibition constants (K i ) and half-maximal inhibitory concentration (IC 50 ) values for chlorpyrifos were determined by Lineweaver-Burk graphs and by plotting activity percentage vs.[I], respectively. Enzyme activities were determined by a colorimetric method. In fish exposed to chlorpyrifos 2.25 and 4.5 µg/L, enzyme inhibition was not observed (p > 0.05); however, chlorpyrifos exposure to 6.75 µg/L for 72 and 96 h caused a significant decrease in enzyme activity (p < 0.05). The IC 50 value of chlorpyrifos was found to be 30 µg/L. These results show that chlorpyrifos may cause direct cellular injury in the brain, and suggest that AChE may be used as a bioindicator for toxicological studies.

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“…], providing information about their inhibitory behaviour and their potential interference in the use of AChE from these species as a biomarker for the presence of anticholinesterase compounds. In our previous studies, AChE from the same species was physicochemical and kinetically characterized and used to investigate the effect of organophosphorus and carbamate pesticides showing sensitivity comparable to a commercial and purified enzyme [14].…”

Section: Objectivementioning

confidence: 99%

“…Enzyme activity was evaluated using an adaptation of Ellman's method according to Assis et al [14]. Briefly, 0.25 mmol/L DTNB (200 μL) prepared in 0.5 mol/L Tris-HCl buffer pH 7.4 was added to the crude extract (20 μL), and the reaction started by the addition of 62 mmol/L acetylthiocholine iodide (20 μL) except for the C. macropomum assays (125 mmol/L).…”

Section: Enzyme Activity and Protein Determinationmentioning

confidence: 99%