Comparative effect of fucoxanthin and vitamin C on oxidative and functional parameters of human lymphocytes

Molina, Nathalia; Morandi, Ana Carolina; Bolin, Anaysa Paola; Otton, Rosemari

doi:10.1016/j.intimp.2014.06.026

Cited by 72 publications

(56 citation statements)

References 69 publications

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“…The vitamin concentrations used were 50 µM for vitamin C, 50 µM for vitamin E, and 500 µM for NADH. Vitamin concentrations used in our study were chosen according to previous studies which showed that these concentrations did not affect cell viability [28] [34] [35]. After incubation for 48 h and centrifugation at 1500 rpm for 10 minutes, the cells were removed and the supernatants were collected for cytokine analysis.…”

Section: Isolation and In Vitro Lymphocyte Proliferation Assaymentioning

confidence: 99%

“…Vitamins C and E are two essential nutrients that can scavenge free radicals and constitute a strong line of defence in retarding ROS-induced cellular damage. Indeed, vitamin C has been shown to increase lymphocyte proliferation, to reduce oxidative damage in cultured human lymphocytes and to protect against cell destruction [26]- [28]. Many studies proved that vitamin E enhanced the immune system, T-cell maturation, lymphocyte proliferation and cytokine production [29] [30].…”

Section: Introductionmentioning

confidence: 99%

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Vitamins C, E, and NADH on <i>in Vitro</i> Lymphocyte Proliferation and Redox Status among Obese Patients

Meraou¹,

Merzouk²,

Saidi³

et al. 2016

FNS

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Background: The prevalence of overweight and obesity associated with oxidative stress and immune abnormalities is continuously increasing. Antioxidant supplementations might counteract potential damage caused by ROS to cellular tissues. Objective: To determine the role of vitamins on immune improvement during obesity, we investigated in vitro effects of vitamins C, E, and NADH on mitogen-stimulated proliferation, Th1-and Th2-type cytokine production, and oxidant/antioxidant status of lymphocytes isolated from obese patients. Methods: Peripheral blood lymphocytes were isolated using a density gradient of Histopaque. They were in vitro cultured and stimulated by Con A in the presence or absence of vitamins. Cell proliferation was determined by MTT assay and interleukin-2, interleukin-4 and interferon-γ (INFγ) secretions. Cell oxidant/antioxidant balance was studied by assaying glutathione (GSH), malondialdehyde (MDA), carbonyl protein levels, catalase activity and micronucli frequency. Results: Obesity is associated with enhanced oxidative stress response. Indeed, vitamin C, E and NADH improved significantly lymphocyte proliferation and diminished cellular oxidative stress. Conclusion: Treatments of lymphocytes with vitamins had beneficial effects on lymphocyte proliferation, cytokines secretions and redox status, generating an anti-inflammatory profile and should be considered in therapeutic approaches for normalizing immune cell function in obesity.

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Section: Isolation and In Vitro Lymphocyte Proliferation Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Vitamins C, E, and NADH on <i>in Vitro</i> Lymphocyte Proliferation and Redox Status among Obese Patients

Meraou¹,

Merzouk²,

Saidi³

et al. 2016

FNS

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show abstract

“…The carotenoids' consumption may protect against UV-induced inflammation by inhibiting apoptosis of keratinocytes and decreasing the erythema's intensity [9,10].…”

Section: Introductionmentioning

confidence: 99%

Fucoxanthin increases lifespan of Drosophila melanogaster and Caenorhabditis elegans

Lashmanova

Proshkina

Zhikrivetskaya

et al. 2015

Pharmacological Research

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“…Interestingly, fucoxanthin and its metabolite fucoxanthinol exhibit few adverse effects on normal, uninfected cells both in vitro and in vivo (Yamamoto et al, 2011). Extensive studies have been carried out regarding the separation, purifi cation, bioavailability, and biological activity of fucoxanthin from macroalgae (Wang et al, 2005;Yang et al, 2008;Fung et al, 2013;Molina et al, 2014;Ye et al, 2014). However, the fucoxanthin content in macroalgae is very low, requiring complex processes for purifi cation and high production costs.…”

Section: Introductionmentioning

confidence: 99%

A comparative analysis of fatty acid composition and fucoxanthin content in six Phaeodactylum tricornutum strains from diff erent origins

Wang

et al. 2015

Chin. J. Ocean. Limnol.

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Phaeodactylum tricornutum is a potential livestock for the combined production of eicosapentaenoic acid (EPA) and fucoxanthin. In this study, six marine diatom strains identifi ed as P . tricornutum were cultured and their total lipid, fatty acid composition and major photosynthetic pigments determined. It was found that the cell dry weight concentration and mean growth rate ranged between 0.24-0.36 g/L and 0.31-0.33/d, respectively. Among the strains, SCSIO771 presented the highest total lipid content, followed by SCSIO828, and the prominent fatty acids in all strains were C16:0, C16:1, C18:1, and C20:5 (EPA). Polyunsaturated fatty acids, including C16:2, C18:2, and EPA, comprised a signifi cant proportion of the total fatty acids. EPA was markedly high in all strains, with the highest in SCSIO828 at 25.65% of total fatty acids. Fucoxanthin was the most abundant pigment in all strains, with the highest in SCSIO828 as well, at 5.50 mg/g. The collective results suggested that strain SCSIO828 could be considered a good candidate for the concurrent production of EPA and fucoxanthin.

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Comparative effect of fucoxanthin and vitamin C on oxidative and functional parameters of human lymphocytes

Cited by 72 publications

References 69 publications

Vitamins C, E, and NADH on <i>in Vitro</i> Lymphocyte Proliferation and Redox Status among Obese Patients

Vitamins C, E, and NADH on <i>in Vitro</i> Lymphocyte Proliferation and Redox Status among Obese Patients

Fucoxanthin increases lifespan of Drosophila melanogaster and Caenorhabditis elegans

A comparative analysis of fatty acid composition and fucoxanthin content in six Phaeodactylum tricornutum strains from diff erent origins

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Comparative effect of fucoxanthin and vitamin C on oxidative and functional parameters of human lymphocytes

Cited by 72 publications

References 69 publications

Vitamins C, E, and NADH on &lt;i&gt;in Vitro&lt;/i&gt; Lymphocyte Proliferation and Redox Status among Obese Patients

Vitamins C, E, and NADH on &lt;i&gt;in Vitro&lt;/i&gt; Lymphocyte Proliferation and Redox Status among Obese Patients

Fucoxanthin increases lifespan of Drosophila melanogaster and Caenorhabditis elegans

A comparative analysis of fatty acid composition and fucoxanthin content in six Phaeodactylum tricornutum strains from diff erent origins

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Product

Resources

About

Vitamins C, E, and NADH on <i>in Vitro</i> Lymphocyte Proliferation and Redox Status among Obese Patients

Vitamins C, E, and NADH on <i>in Vitro</i> Lymphocyte Proliferation and Redox Status among Obese Patients