Comparative Determination of Bacitracin by HPLC and Microbiological Methods in Some Pharmaceuticals and Feed Grade Preparations

Fast separation of bacitracin on monolithic silica columns

“…The separation of B1 and B2 was not achieved even with the mobile phase composed only of methanol and phosphate buffer (Fig. 3a), which proved to be effective for separation of mentioned peak pairs in our previous study performed on conventional column [8,10]. It is worth mentioning that the separation of peaks B1 and B2 is most difficult to achieve as recognised by other authors, too [5].…”

“…As optimal concentration and pH of phosphate buffer in the mobile phase had already been determined previously on conventional columns [10,13], we tried to improve the selectivity of Bc separation by changing the ratio between methanol and acetonitrile in the mobile phase. The ratio between organic phase and buffer was kept constant 50:50…”

Pathways of Chemical Degradation of Polypeptide Antibiotic Bacitracin

“…F splits into FЈ and FЉ). 8,25) A pronounced splitting of peaks of oxidative degradation products H2, H3 and F, with the exception of H1, was observed on conventional reversed phase HPLC columns when acidic mobile phase with pH 2 was used (Fig. 4).…”

Electrochemical Properties of Membranes Based on Associates of Beta-Lactam Antibiotics with Tetradecilammonium

“…There exist various physicochemical drug assay methods [2][3][4]. A promising method of the assay of antibacterial substances in biological and medicinal media is potentiometry with ion-selective electrodes (ISE).…”