Pathways of Chemical Degradation of Polypeptide Antibiotic Bacitracin

Pavli, Viljem; Kmetec, Vojko

doi:10.1248/bpb.29.2160

Cited by 17 publications

(10 citation statements)

References 16 publications

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“…Chlortetracycline, bacitracin A, bacitracin F, fenbendazole, and tylosin were included in the analyses. At near neutral pH, bacitracin A rapidly degrades to multiple relatively unstable products through oxidation and hydrolysis on exposure to air and water (Pavli and Kmetec, 2006). One of the more stable degradates in water is bacitracin F (Pavli et al, 2004).…”

Section: Methodsmentioning

confidence: 99%

Narrow Grass Hedges Reduce Tylosin and Associated Antimicrobial Resistance Genes in Agricultural Runoff

Soni

Bartelt–Hunt

Snow³

et al. 2015

J. Environ. Qual.

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Agricultural runoff from areas receiving livestock manure can potentially contaminate surface water with antimicrobials and antimicrobial resistance genes (ARGs). The objective of this study was to investigate the effectiveness of narrow grass hedges (NGHs) on reducing the transport of antimicrobials and ARGs in runoff after land application of swine manure slurry. Plot-scale rainfall simulation tests were conducted on 0.75 m by 4.0 m plots designed to test three treatment factors: manure amendment (control plots receiving no manure vs. amended plots receiving manure based on 3 times N requirement), NGH (plots with a NGH vs. plots without a NGH), and rainfall events (days 1-3). Runoff generated during three 30-min simulated rainfall events was sampled and analyzed for antimicrobials and ARGs. Manure amendment was responsible for the presence of antimicrobial tylosin ( < 0.0001) and tylosin resistance gene (B) ( < 0.0001) in runoff. Narrow grass hedges proved to be effective in reducing tylosin ( < 0.0001) and (B) ( < 0.0347) in runoff. Manure amendment was responsible for the introduction of tylosin ( < 0.0482) and (B) ( = 0.0128) into the soil; however, it had no significant impact on the abundance of the 16S rRNA gene in soil. Results from this study suggest that NGHs could be a best management practice to control the transport of antimicrobials and ARGs in agricultural runoff.

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Section: Methodsmentioning

confidence: 99%

Narrow Grass Hedges Reduce Tylosin and Associated Antimicrobial Resistance Genes in Agricultural Runoff

Soni

Bartelt–Hunt

Snow³

et al. 2015

J. Environ. Qual.

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“…This potent antibiotic is currently used in some topical preparations both in human and veterinary medicine and has also been tested, with limited success, for elimination of vancomycin-resistant enterococci [5]. Bacitracin is not administered systemically owing to its nephrotoxicity but is considered safe when taken orally as the gastrointestinal tract does not absorb significant amounts of the drug [6,7].…”

Section: Introductionmentioning

confidence: 99%

Study on the dissemination of the bcrABDR cluster in Enterococcus spp. reveals that the BcrAB transporter is sufficient to confer high-level bacitracin resistance

Matos

Pinto

Ruivo

et al. 2009

International Journal of Antimicrobial Agents

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Bacitracin is used both in clinical practice and as an animal growth promoter in some countries. Recent reports revealed the dissemination of high-level bacitracin resistance (HLBR) in the genus Enterococcus. The bcrABD operon confers HLBR in an Enterococcus faecalis strain, and the presence of the regulator BcrR was stated as essential for this phenotype. Our objectives were to investigate the spread of the bcrABDR genes in the genus Enterococcus and to evaluate the role of this genotype in the HLBR phenotype. Fifty-eight enterococcal isolates from different environments and species were screened for the presence of the bcrABDR genes, and minimum inhibitory concentrations for bacitracin were determined. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to study the expression of bacitracin resistance genes in strains with HLBR following growth in the presence and absence of the antibiotic. The bcrABDR genotype was found associated with E. faecalis strains from clinical settings bearing a HLBR phenotype. The most disseminated mechanism of bacitracin resistance was the BcrAB ABC transporter, encoded by the bcrAB genes. This transporter, which is constitutively expressed in the absence of bcrR, was sufficient to confer HLBR. Our findings stress the need for further evaluation of bacitracin resistance in this genus.

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“…Although several bacitracins have been described in the literature, the most thoroughly researched is bacitracin A (7), a cyclic decapeptide with four of the aminoacids (glutamic, ornithine, phenylalanine, and asparagine) in the D-configuration. The molecule contains a thiazoline ring structure and exhibits potent antibiotic activity against Gram-positive bacteria (Pavli and Kmetec 2006). Other decapeptides Microorganisms isolated from marine habitats have emerged as a promising source of new bioactive metabolites with potential for development into drugs for treating human diseases (Davidson 1995).…”

Section: Bacitracin Familymentioning

confidence: 99%

Comparative genome analysis of Bacillus spp. and its relationship with bioactive nonribosomal peptide production

et al. 2013

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Bacillus genus comprises an important number of species which produce a wide range of secondary metabolites displaying a broad spectrum of activity and great structural diversity. The genome sequences of an important number of species have been published and a large number of orphan genes reported. This review, covering all the literature in this field up to end of 2011, summarizes and compares the genetic potential of these organisms from the point of view of bioactive nonribosomal peptide production and their application as antibiotics, plant pathogen biocontrol, promotion of plant growth, etc. The biological and structural studies of the peptides isolated from Bacillus species are revised and some aspects of the biosynthesis of these metabolites and related compounds are discussed.

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Pathways of Chemical Degradation of Polypeptide Antibiotic Bacitracin

Cited by 17 publications

References 16 publications

Narrow Grass Hedges Reduce Tylosin and Associated Antimicrobial Resistance Genes in Agricultural Runoff

Narrow Grass Hedges Reduce Tylosin and Associated Antimicrobial Resistance Genes in Agricultural Runoff

Study on the dissemination of the bcrABDR cluster in Enterococcus spp. reveals that the BcrAB transporter is sufficient to confer high-level bacitracin resistance

Comparative genome analysis of Bacillus spp. and its relationship with bioactive nonribosomal peptide production

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