Comparative detection of mite allergens in house dust of homes in Moscow by enzyme‐linked immunosorbent assay and acarologic analysis

Abstract

The present study revealed that 73% of surveyed apartments in Moscow whose residents included children with the atopic form of bronchial asthma and sensitization to Dermatophagoides pteronyssinus allergens were infested with the pyroglyphid mites D. pteronyssinus and D. farinae. The number of mites in the surveyed apartments varied between 0 and 154 mites/g of dust for D. pteronyssinus and between 0 and 162 mites/g of dust for D. farinae. The levels of mite allergens in these apartments ranged from 0.5 to 165.8 micrograms/g for Der p I and from 0.3 to 91.3 micrograms/g of dust for Der f I. The Der p I allergen was found to predominate, and its concentration in one-third of the apartments was more than 10-fold greater than that of Der f I. Correlation between the number of pyroglyphid mites and the concentration of group I allergens was established for both D. pteronyssinus (r = 0.4932; P < 0.01) and D. farinae (r = 0.6748; P < 0.01). In most of the apartments, high and moderate levels of Der I allergens were detected.

Environmental assessment and exposure control of dust mites: a practice parameter

“…A strong correlation was found between the number of mites and the concentrations of Der p 1 and Der f 1. 195 …”

Presence and density of domestic mites in the microenvironment of mite‐sensitive dogs with atopic dermatitis

“…Another interesting finding was the relatively low median DM density per gram of dust in group A households (8.8), owner mattress (7.4) and dog sleeping area (8). In human eczema, presence of ≥100 DMs/g of dust increases the possibility of sensitization, while in asthma ≥500 DMs/g of dust is needed for the same purpose 14,23–27 …”

“…In particular, the median number of DMs has been estimated as 32/g of dust in Sweden, 27 and more than 100 DMs/g of dust in 83.3% of asthmatic patient households in the USA 23 . Interestingly, the generally accepted cut‐off concentration of DM allergens which is needed for the clinical expression of allergic diseases in humans (2 μg/g of dust) 14,24,25,27 was found in only 6–42% of dust samples collected from households with atopic dogs in Austria and the UK 20,45 . Although regional differences may explain some of these findings, we hypothesize that lower DM density is needed for the sensitization and clinical expression of CAD than for human asthma.…”

“…In atopic humans, a relationship between exposure to DM allergens and sensitization or allergic clinical manifestations has been proposed; presence of ≥100 and of ≥500 DMs/g of dust increases the possibility of sensitization and asthma induction, respectively 14,23–27 . Whether the same is true in CAD is still unknown, although in one study the concentrations of DM allergens did not differ between households with atopic and healthy dogs 20 …”

Biodiversity And Population Dynamics Of Dust Mites

“…Relative humidity also affects the development and growth of the mite population (Zheltikova. 1985) [30]. Tilak and Jogdand (1989) [18] worked on the impact of environment on the occurrence of house dust mites in different localities of Aurangabad and reported highest percent contribution of house dust mite is recorded during rainy season in the month of September under congenial environmental conditions, when the temperature was around 25 0 C and range of humidity was between 75% and 85%.…”