Comparative Cytotoxicity of <scp><i>Glycyrrhiza glabra</i></scp> Roots from Different Geographical Origins Against Immortal Human Keratinocyte (HaCaT), Lung Adenocarcinoma (A549) and Liver Carcinoma (HepG2) Cells

Basar, Norazah; Oridupa, Olayinka A.; Ritchie, Kenneth J.; Nahar, Lutfun; Osman, Nashwa; Stafford, Angela; Kushiev, Kh. Kh.; Kan, Asuman; Sarker, Satyajit D.

doi:10.1002/ptr.5329

Cited by 35 publications

(26 citation statements)

References 22 publications

(30 reference statements)

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“…Cell lines, cell cultures and the 3-(4,5-dimethylthiazol-2-yl)-2,5-dphenyltetrazolium bromide assay. The potential cytotoxicity of C. reticulata stem bark extracts, their VLC fractions and the isolated compounds were studied against cancer cell lines A549 (human lung adenocarcinoma), MCF7 (human breast adenocarcinoma), PC3 (human prostate adenocarcinoma) and the normal cell line PNT2 (human prostate) using the MTT assay (Mosmann, 1983;Basar, Oridupa, Ritchie, et al, 2015). All cell lines were cultured in RPMI-1640 medium supplemented with 10% foetal bovine serum and 1% antibiotic-antimycotic solution (100 ×).…”

Section: Methodsmentioning

confidence: 99%

Cytotoxic Properties of the Stem Bark ofCitrus reticulataBlanco (Rutaceae)

et al. 2017

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The bioassay-guided fractionation of the n-hexane extract of Citrus reticulata Blanco (Rutaceae) stem bark yielded scoparone (1), xanthyletin (2), lupeol (3), β-amyrin (4), stigmasterol (5), β-sitosterol (6) and palmitic acid. The structures of these compounds were determined by comprehensive spectroscopic analyses, i.e., 1D and 2D NMR and EI-MS, and by comparison with the reported data. Extracts, fractions and isolated compounds 1-6 were assessed for cytotoxicity by the 3-(4,5-dimethylthiazol-2-yl)-2,5-dphenyltetrazolium bromide (MTT) assay against three human cancer cell lines, i.e., human lung adenocarcinoma cell line A549, human breast adenocarcinoma cell line MCF7 and human Caucasian prostate adenocarcinoma cell line PC3. Significant activity of the n-hexane and the dichloromethane extracts was observed against the breast cancer cell line MCF7 with IC s of 45.6 and 54.7 μg/mL, respectively. Moreover, the 70% ethyl acetate in n-hexane chromatographic fraction showed significant activity displaying IC values of 53.0, 52.4 and 49.1 μg/mL against the cancer cell lines A549, MCF7 and PC3, respectively. Encouragingly, an IC of 510.0 μg/mL against the human normal prostate cell line PNT2 indicated very low toxicity and hence favourable selectivity indices for the 70% ethyl acetate in n-hexane fraction in the range of 9.6-10.4 towards cell lines A549, MCF7 and PC3. Because compounds isolated from the above fraction only delivered IC values in the range of 18.2-96.3, 9.2-34.1 and 7.5-97.2 μg/mL against A549, MCF7 and PC3 cell lines, respectively, synergistic action between compounds is suggested. Bioassay results valorize the anticancer effectivity of the stem bark of this plant in Cameroonian pharmacopoeia. Copyright © 2017 John Wiley & Sons, Ltd.

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Section: Methodsmentioning

confidence: 99%

Cytotoxic Properties of the Stem Bark ofCitrus reticulataBlanco (Rutaceae)

et al. 2017

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“…Briefly, RD cells suspension (100 µL) were seeded into 96-well plates and maintained in the incubator for 24 h. Subsequently, the cells, which obtained a confluency of about 90%, were treated with serial dilutions (100 µL each) of the mushroom extract, ranging from 1000 -0.01 µg/mL. The extract itself was previously dissolved in DMSO and diluted with the culture medium, ensuring the maximum concentration of DMSO was not more than 0.05%, a concentration that is not toxic to cancer cells 30 . The treatment was carried out for a period of 72 h in the same environment mentioned above.…”

Section: Mtt (3-(45-dimethylthiazol-2-yl)-25-diphenyl Tetrazolium) mentioning

confidence: 99%

Antiprotozoal, antiviral and cytotoxic properties of the nigerian mushroom, hypoxylon fuscum pers. fr. (xylariaceae)

Ogbole¹,

Segun²,

Akinleye³

et al. 2018

actapharm

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In Nigeria, mushrooms are utilised for the treatment of several ailments. Although the biological activities of some Nigerian mushrooms have been reported, there is dearth of information on the therapeutic potentials of Hypoxylon fuscum. The aim of this study is to investigate the antileishmanial, antiplasmodial, antitrypanosomal, cytotoxic and antiviral activities of H. fuscum. The antiplasmodial studies revealed that the extract was active against chloroquine-sensitive D6 and chloroquine-resistant W2 strains of Plasmodium falciparum (IC 50 of 6.98 and 8.33 µg/ mL, respectively). The extract showed antitrypanosomal activity on Trypanosoma brucei brucei but lacked inhibitory activity against Leishmania donovani. The extract displayed cytotoxicity on Artemia salina larvae and rhabdomyosarcoma cell line, with CC 50 value of 3.33 and 8.60 µg/mL, respectively, and also displayed antiviral activity on echoviruses (E7 and E19). This study demonstrated that Hypoxylon fuscum possess several pharmacological activities and may provide a drug lead for the development of effective chemotherapeutic agents.

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“…Researchers have evaluated the cytotoxicity of polyphenol molecule extracted from Glycyrrhiza glabra which is used for the treatment of breast and prostate cancer, head and neck cancer cell lines and non‐malignant primary mucosal cells, and against different cancer cell lines such as Immortal Human Keratinocyte (HaCaT), Lung Adenocarcinoma (A549), and Liver Carcinoma (HepG2) cell lines …”

Section: Resultsmentioning

confidence: 99%

Characterization and anti‐acute T cell leukemia properties of silver nanoparticles synthesized by a green approach for bioremediation applications: Introducing a new chemotherapeutic drug for clinical trial studies

Ahmeda

Zangeneh

2020

Applied Organom Chemis

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In recent years, biosynthesis of silver nanoparticles using plant extracts and bioactive compounds has gained much attention. Green synthesis of nanoparticles is eco‐friendly, cost‐effective, and is a promising substitute for the chemical synthesis of silver nanoparticles. Also, the previous experiments have revealed that plants raise the cytotoxicity potentials of silver nanoparticles against several cell lines especially tumor cell lines. The present study confirms the potential of aqueous extract of Glycyrrhiza glabra L leaf grown under in vitro condition for the biosynthesis of silver nanoparticles (AgNPs). Also, in this study, we revealed the antioxidant and anti‐acute T cell leukemia properties of AgNPs against J45.01, Jurkat, Clone E6–1, J.CaM1.6, and J.RT3‐T3.5 cell lines. Silver nanoparticles were characterized and analyzed using common nanotechnology techniques including UV–Vis. and FT‐IR spectroscopy, TEM, and FE‐SEM. DPPH free radical scavenging test was carried out to assess the antioxidant potentials of AgNO3, G. glabra, and AgNPs. MTT assay was used on normal cell line (HUVEC) and acute T cell leukemia cell lines (J45.01, Jurkat, Clone E6–1, J.CaM1.6, and J.RT3‐T3.5) for analyzing of anti‐acute T cell leukemia properties of AgNO3, G. glabra, and AgNPs. FT‐IR analysis revealed antioxidant compounds in the nanoparticles were the sources of reducing power, reducing silver ions to AgNPs. TEM and FE‐SEM images indicated a uniform spherical morphology in size of 20 nm for silver nanoparticles. DPPH test indicated similar antioxidant activities for G. glabra, AgNPs, and butylated hydroxytoluene. Silver nanoparticles had very low cell viability dose‐dependently against J45.01, Jurkat, Clone E6–1, J.CaM1.6, and J.RT3‐T3.5 cell lines without any cytotoxicity on HUVEC cell line. The best result of cytotoxicity property of AgNPs against above cell lines was seen in the case of J.CaM1.6 cell line. Above findings confirm the significant antioxidant and anti‐acute T cell leukemia properties of AgNPs. After confirming these results in clinical trial studies, AgNPs can be used as a chemotherapeutic drug for the treatment of acute T cell leukemia in human.

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Comparative Cytotoxicity of Glycyrrhiza glabra Roots from Different Geographical Origins Against Immortal Human Keratinocyte (HaCaT), Lung Adenocarcinoma (A549) and Liver Carcinoma (HepG2) Cells

Cited by 35 publications

References 22 publications

Cytotoxic Properties of the Stem Bark ofCitrus reticulataBlanco (Rutaceae)

Cytotoxic Properties of the Stem Bark ofCitrus reticulataBlanco (Rutaceae)

Antiprotozoal, antiviral and cytotoxic properties of the nigerian mushroom, hypoxylon fuscum pers. fr. (xylariaceae)

Characterization and anti‐acute T cell leukemia properties of silver nanoparticles synthesized by a green approach for bioremediation applications: Introducing a new chemotherapeutic drug for clinical trial studies

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