Comparative Cytogenetics of Wild Crucifers (Brassicaceae)

Lysak, Martin A.

doi:10.1201/9781420086096.ch10

Cited by 8 publications

(14 citation statements)

References 152 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our molecular cytogenetic map was able to identify the corresponding homoeologous chromosomes or regions between the A and C genomes, and we optimized hybridization conditions to avoid detecting ancient paralogous segments indicative of ancient polyploidy events (Lysak et al 2005;Lysak 2009). Most of our physical mapping results were consistent with the published genetic map of B. napus (Parkin et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Using the karyotype approach presented here, individual BAC clones can be accurately localized in detail to chromosomes and linkage groups using FISH. Because the Brassica genomes have undergone ancient whole-genome duplications that are detectable using Arabidopsis BAC clones in a comparative chromosome painting approach (Lysak et al 2005;Lysak 2009), hybridization conditions were optimized to select against visualization of those paralogs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Karyotype and Identification of All Homoeologous Chromosomes of AllopolyploidBrassica napusand Its Diploid Progenitors

2011

View full text Add to dashboard Cite

Investigating recombination of homoeologous chromosomes in allopolyploid species is central to understanding plant breeding and evolution. However, examining chromosome pairing in the allotetraploid Brassica napus has been hampered by the lack of chromosome-specific molecular probes. In this study, we establish the identification of all homoeologous chromosomes of allopolyploid B. napus by using robust molecular cytogenetic karyotypes developed for the progenitor species Brassica rapa (A genome) and Brassica oleracea (C genome). The identification of every chromosome among these three Brassica species utilized genetically mapped bacterial artificial chromosomes (BACs) from B. rapa as probes for fluorescent in situ hybridization (FISH). With this BAC-FISH data, a second karyotype was developed using two BACs that contained repetitive DNA sequences and the ubiquitous ribosomal and pericentromere repeats. Using this diagnostic probe mix and a BAC that contained a C-genome repeat in two successive hybridizations allowed for routine identification of the corresponding homoeologous chromosomes between the A and C genomes of B. napus. When applied to the B. napus cultivar Stellar, we detected one chromosomal rearrangement relative to the parental karyotypes. This robust novel chromosomal painting technique will have biological applications for the understanding of chromosome pairing, homoeologous recombination, and genome evolution in the genus Brassica and will facilitate new applied breeding technologies that rely upon identification of chromosomes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Karyotype and Identification of All Homoeologous Chromosomes of AllopolyploidBrassica napusand Its Diploid Progenitors

2011

View full text Add to dashboard Cite

show abstract

“…In addition, our study is similar to those that used BAC-FISH across species (comparative chromosome painting or CCP) [reviewed in Lysak, 2009 andLysak et al, 2010]. Comparative BAC-FISH across species has been done recently in Solanaceae [Tang et al, 2008] and Cucurbitaceae [Han et al, 2009], but has a longer history among taxa of the Poaceae and the Brassicaceae.…”

Section: Discussionmentioning

confidence: 50%

“…In the Poaceae, Sorghum or Brachypodium BACS have been used to integrate the genetic and physical maps of grass genera with larger genomes [Draye et al, 2001, Bowers et al, 2003, Koumbaris and Bass, 2003Kim et al, 2005;Hasterok et al, 2006;Ina et al, 2007;Gu et al, 2009]. In the Brassicaceae, A. thaliana BACs have been used in comparative chromosome painting analyses [Lysak et al, 2001[Lysak et al, , 2003[Lysak et al, , 2010Mandáková and Lysak, 2008;Lysak, 2009], and recently a mixture of 2 species of BACs has been used to further facilitate comparative BAC-FISH analyses [Lysak et al, 2010]. To our knowledge, our study of B. rapa chromosome A7 is the first to hybridize B. rapa BACs onto both B. rapa chromosomes ( fig.…”

Section: Discussionmentioning

confidence: 99%

“…However, among rosids, there have been numerous studies in legumes (Fabaceae) [Kulikova et al 2001;Walling et al, 2006;PedrosaHarand, 2009], cotton (Malvaceae) [Wang et al, 2007a[Wang et al, , b, c, 2008Guo et al, 2008], Populus (Salicaceae) [IslamFaridi et al, 2009], Poncirus (Rutaceae) [Moraes et al, 2008], cucurbits (Cucurbitaceae) [Han et al, 2009], and mustards (Brassicaceae). Given the small genomes of Brassicaceae and the early sequencing of Arabidopsis thaliana , it is perhaps no surprise that the largest number of BAC-FISH studies has been conducted in the mustard family [Jackson et al, 1998[Jackson et al, , 2000Lysak et al, 2001Lysak et al, , 2003Lysak et al, , 2006Lysak et al, , 2010Howell et al, 2002Howell et al, , 2005Howell et al, , 2008Ziolkowski et al, 2006;Mandáková and Lysak, 2008;Lysak, 2009].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Integration of Genetic, Physical, and Cytogenetic Maps for <i>Brassica rapa</i> Chromosome A7

Xiong

Kim

Pires

2010

Cytogenet Genome Res

View full text Add to dashboard Cite

Bacterial artificial chromosome (BAC) contigs have been genetically mapped to the 10 linkage groups of Brassica rapa by BAC end sequences (BES). To integrate the genetic, physical, and cytogenetic maps, fluorescence in situ hybridization (FISH) was used to anchor the assembly of BAC contigs onto Brassica chromosomes using representative BACs. This BAC-FISH approach can be used to identify chromosome arms on separate mitotic metaphase chromosomes or to map multiple BACs to single long pachytene chromosomes. As part of an international consortium that is sequencing the B. rapa genome, we integrated the linkage and physical maps with the B. rapa cytogenetic map for chromosome A7 by hybridizing BACs to mitotic chromosomes and along the length of pachytene chromosome spreads. A total of 31 BACs that were putatively located on A7 were used as probes for FISH analyses; however, only 19 BACs mapped unambiguously to A7 while the remaining BACs either mapped to other chromosomes or hybridized to multiple locations. We then created a multicolor FISH cocktail of 16 BAC probes to simultaneously hybridize the entire length of the A7 chromosome. We successfully applied the 16 A7 BAC probe mix to B. rapa, B. oleracea, and domesticated and resynthesized genotypes of B. napus to demonstrate that this approach can facilitate studies of genome evolution by integrating the genetic, physical, and cytogenetic maps among closely related species of Brassica.

show abstract

Brassicaceae (Mustard Family)

Al‐Shehbaz

2011

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

The Brassicaceae (Cruciferae) or mustard family is a well‐defined group of about 310 genera and some 3500 species distributed primarily in the temperate and alpine areas of all continents except Antarctia. It is most highly diversified in central and western Asia, Mediterranean Europe and western North America. This article discusses the economic importance of the family (food, oils, condiments, ornamentals and weeds) and role played by one of its weedy member, the model organism Arabidopsis thaliana , in the advancement of modern experimental biology. Here we also review the morphology, biogeography, ecology and phylogenetic relationship within Brassicaceae and to other families. We also cover the significance of molecular data in dividing the family into 48 monophyletic tribes and in determining its major lineages. Finally, a brief discussion is presented on the whole‐genome duplication events and their possible role in the radiation and diversification of the family. Key Concepts: Model organisms, such as the mustard Arabidopsis thaliana , should always be used to understand complex biological phenomena in other organisms. Owing to extensive morphological convergence, molecular data alone provide the most reliable and solid phylogenetic classification within the Brassicaceae. Invasive weeds of the mustard family can only be understood by closer studies of their immediate wild relatives. Germplasm conservation centres of the economically important Brassicaceae should substantially increase their efforts to save their rapidly disappearing wild relatives. Vegetable crops of Brassica should be thoroughly tested to determine their medicinal values in combating cancer and other diseases.

show abstract

Comparative Cytogenetics of Wild Crucifers (Brassicaceae)

Cited by 8 publications

References 152 publications

Karyotype and Identification of All Homoeologous Chromosomes of AllopolyploidBrassica napusand Its Diploid Progenitors

Karyotype and Identification of All Homoeologous Chromosomes of AllopolyploidBrassica napusand Its Diploid Progenitors

Integration of Genetic, Physical, and Cytogenetic Maps for <i>Brassica rapa</i> Chromosome A7

Brassicaceae (Mustard Family)

Contact Info

Product

Resources

About