Integration of Genetic, Physical, and Cytogenetic Maps for &lt;i&gt;Brassica rapa&lt;/i&gt; Chromosome A7

Xiong, Zhiyong; Kim, J. S.; Pires, J. Chris

doi:10.1159/000314640

Cited by 20 publications

(16 citation statements)

References 187 publications

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“…Among the crop species in the Brassicaceae, the B. rapa genetic, physical, and cytogenetic maps have begun to be integrated by BAC-FISH (Koo et al 2004;Lim et al 2005Lim et al , 2007Yang et al 2007;Kim et al 2009;Mun et al 2009;Xiong et al 2010). However, to our knowledge, a complete karyotype analysis that reliably distinguishes each chromosome in B. oleracea and B. napus has not been reported.…”

Section: Discussionmentioning

confidence: 99%

“…These results suggested that an ancient chromosome rearrangement occurred between the two duplicated regions of C4B in either the A or C genomes after the diversification from a common ancestor. The future of plant molecular cytogenetics is promising in terms of both methods and applications (Kato et al 2005;Walling et al 2005;Lamb and Birchler 2006;Danilova and Birchler 2008;Guerra 2008;Pires and Hertweck 2008;Lysak et al 2010;Xiong et al 2010). The robust novel chromosomal painting technique developed here will assist the understanding of chromosome pairing, homoeologous recombination, and genome evolution in the genus Brassica and will facilitate new applied breeding technologies in this genus that rely upon identification of individual chromosomes.…”

Section: Discussionmentioning

confidence: 99%

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Karyotype and Identification of All Homoeologous Chromosomes of AllopolyploidBrassica napusand Its Diploid Progenitors

2011

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Investigating recombination of homoeologous chromosomes in allopolyploid species is central to understanding plant breeding and evolution. However, examining chromosome pairing in the allotetraploid Brassica napus has been hampered by the lack of chromosome-specific molecular probes. In this study, we establish the identification of all homoeologous chromosomes of allopolyploid B. napus by using robust molecular cytogenetic karyotypes developed for the progenitor species Brassica rapa (A genome) and Brassica oleracea (C genome). The identification of every chromosome among these three Brassica species utilized genetically mapped bacterial artificial chromosomes (BACs) from B. rapa as probes for fluorescent in situ hybridization (FISH). With this BAC-FISH data, a second karyotype was developed using two BACs that contained repetitive DNA sequences and the ubiquitous ribosomal and pericentromere repeats. Using this diagnostic probe mix and a BAC that contained a C-genome repeat in two successive hybridizations allowed for routine identification of the corresponding homoeologous chromosomes between the A and C genomes of B. napus. When applied to the B. napus cultivar Stellar, we detected one chromosomal rearrangement relative to the parental karyotypes. This robust novel chromosomal painting technique will have biological applications for the understanding of chromosome pairing, homoeologous recombination, and genome evolution in the genus Brassica and will facilitate new applied breeding technologies that rely upon identification of chromosomes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Karyotype and Identification of All Homoeologous Chromosomes of AllopolyploidBrassica napusand Its Diploid Progenitors

2011

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“…For this, BAC fluorescence in situ hybridization (FISH) experiments were performed using some of the BAC clones identified by Xiong et al (2010), which specifically and simultaneously label pairs of homoeologous chromosomes/regions. We first focused on the A1-C1 pair of homoeologs (Figure 7), which are collinear along their entire length (Parkin et al, 2005).…”

Section: The Dynamics Of Class I Co Formation Is Different Between Thmentioning

confidence: 99%

“…We then extended our BAC FISH survey to three other pairs of chromosomes that share homoeology only along one of their arms, i.e., the long arms of A3/C3, the long arms of A7/C6, and the long arm of A10/short arm of C9 (Parkin et al, , 2005Xiong et al, 2010). Every pair showed a distinct and specific pattern at metaphase I (Supplemental Figure 13): A10 and C9 recombined together much more frequently in Darmor-bzh than in Yudal allohaploids (Table 3; P = 0.0095); A7 and C6 recombined together in the same proportion of PMCs in Darmor-bzh and Yudal allohaploids ( (Table 3; P = 0.1915).…”

Section: The Dynamics Of Class I Co Formation Is Different Between Thmentioning

confidence: 99%

Homoeologous Chromosome Sorting and Progression of Meiotic Recombination inBrassica napus: Ploidy Does Matter!

et al. 2014

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Meiotic recombination is the fundamental process that produces balanced gametes and generates diversity within species. For successful meiosis, crossovers must form between homologous chromosomes. This condition is more difficult to fulfill in allopolyploid species, which have more than two sets of related chromosomes (homoeologs). Here, we investigated the formation, progression, and completion of several key hallmarks of meiosis in Brassica napus (AACC), a young polyphyletic allotetraploid crop species with closely related homoeologous chromosomes. Altogether, our results demonstrate a precocious and efficient sorting of homologous versus homoeologous chromosomes during early prophase I in two representative B. napus accessions that otherwise show a genotypic difference in the progression of homologous recombination. More strikingly, our detailed comparison of meiosis in near isogenic allohaploid and euploid plants showed that the mechanism(s) promoting efficient chromosome sorting in euploids is adjusted to promote crossover formation between homoeologs in allohaploids. This suggests that, in contrast to other polyploid species, chromosome sorting is context dependent in B. napus.

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“…One of them is FISH with bacterial artificial chromosome (BAC) clones selected from a BAC contig physical map (Tang et al 2009;Xiong et al 2010). Another attractive approach is to isolate chromosomes and use them as template for polymerase chain reaction (PCR) with primers specific for molecular markers.…”

Section: Introductionmentioning

confidence: 99%

Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes

et al. 2011

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Cultivated chickpea is the third most important legume after field bean and garden pea worldwide. Despite considerable breeding towards improved yield and resistance to biotic and abiotic stresses, the production of chickpea remained stagnant, but molecular tools are expected to increase the impact of current improvement programs. As a first step towards this goal, various genetic linkage maps have been established and markers linked to resistance genes been identified. However, until now, only one linkage group (LG) has been assigned to a specific chromosome. In the present work, mitotic chromosomes were sorted using flow cytometry and used as template for PCR with primers designed for genomic regions flanking microsatellites. These primers amplify sequence-tagged microsatellite site markers. This approach confirmed the assignment of LG8 to the smallest chromosome H. For the first time, LG5 was linked to the largest chromosome A, LG4 to a medium-sized chromosome E, while LG3 was anchored to the second largest chromosome B. Chromosomes C and D could not be flow-sorted separately and were jointly associated to LG6 and LG7. By the same token, chromosomes F and G were anchored to LG1 and LG2. To establish a set of preferably diagnostic cytogenetic markers, the genomic distribution of various probes was verified using FISH. Moreover, a partial genomic bacterial artificial chromosome (BAC) library was constructed and putative single/low-copy BAC clones were mapped cytogenetically. As a result, two clones were identified localizing specifically to chromosomes E and H, for which no cytogenetic markers were yet available.

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Integration of Genetic, Physical, and Cytogenetic Maps for <i>Brassica rapa</i> Chromosome A7

Cited by 20 publications

References 187 publications

Karyotype and Identification of All Homoeologous Chromosomes of AllopolyploidBrassica napusand Its Diploid Progenitors

Karyotype and Identification of All Homoeologous Chromosomes of AllopolyploidBrassica napusand Its Diploid Progenitors

Homoeologous Chromosome Sorting and Progression of Meiotic Recombination inBrassica napus: Ploidy Does Matter!

Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes

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