Comparative computational RNA analysis of cardiac-derived progenitor cells and their extracellular vesicles

Computational analysis of serum-derived extracellular vesicle miRNAs in juvenile sheep model of single stage Fontan procedure

“…Among them, RNAs carried by EVs have pleiotropic effects including immunomodulation, fibrosis, apoptosis, and proliferative functions in several cardiac diseases. 14 The number of RNA reads in EV post-surgery had also increased in two of the three sheep ( Fig. 4a ), indicating circulating EVs may protect internal cargo from degradation in the blood.…”

“…Sequence reads were mapped and annotated by the Qiagen Gene-Globe console with QIAseq miRNA Quantification tool as previously described. 14 Subsequentially, adapters from 3′ and 5′ ends were trimmed using cutadapt, reads were cleaned by removing sequences less than 16 base pairs. Cleaned reads were aligned with Bowtie aligner to GRCh38 and miRBase v21 references.…”

“… 18 Targets validated by at least three experimental methods were used for biological pathway enrichment analyses using Metascape. 14 , 18 Metascape was further used to identify miRNA target cells.…”

Charting the Path: Navigating Embryonic Development to Potentially Safeguard against Congenital Heart Defects

“…In addition to their differentiation abilities, stem cells secrete a wide range of bioactive molecules (secretomes) and release extracellular vesicles, including exosomes, which have promising potential in facilitating the repair and regeneration of damaged adult cardiac tissues and improving heart function [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. The secretomes and exosomes released by various cells have significant roles in (cardiac) development and tissue repair, and hold promising potential toward reversing CHDs, or at least mitigating their severity [ 19 , 37 , 38 , 39 , 40 , 41 ].…”

Statistical modeling of extracellular vesicle cargo to predict clinical trial outcomes for hypoplastic left heart syndrome

“…We and others have shown that stem and progenitor cells are enriched in certain RNAs that promote processes such as cell proliferation, cell migration, and angiogenesis, that may drive therapeutic success of cell therapy preclinical models. 13 , 15 , 16 , 17 , 18 , 19 To better understand the mechanistic EV RNA signals driving reparative outcomes, we have previously built regression models to link EV RNA-sequencing data to in vitro and in vivo experimental outcomes. 7 , 9 , 13 , 16 Here, we aim to build upon this work, expanding our overall sample size and collecting results for clinical trial samples.…”