Comparative clinical study of canine and feline total blood cell count results with seven in‐clinic and two commercial laboratory hematology analyzers

Becker, Martina; Moritz, Andreas; Giger, Urs

doi:10.1111/j.1939-165x.2008.00085.x

Cited by 55 publications

(93 citation statements)

References 17 publications

(30 reference statements)

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“…In previous studies comparing impedance and flow cytometrybased platelet counts in cats, both comparatively higher and lower impedance-based counts have been reported. 2,5,24 Correlations for baseline and postagonist platelet counts between the 2 analyzers ranged from 0.70 to 0.77 (Table 2), likely reflecting some imprecision of platelet counts by both analyzers. A limitation of our study is that only 1 value with the flow cytometry-based analyzer was obtained.…”

Section: Discussionmentioning

confidence: 99%

“…The mean (range) baseline platelet counts by flow cytometry in our study were similar to previous studies on a similar analyzer, which reported a mean of 281 × 10 9 /liter, with 2.5th and 97.5th percentile of 42 and 630 × 10 9 /liter, respectively, and a mean (range) of 289 (16-858) × 10 9 /liter, using EDTAanticoagulated blood. 5,30 The RI provided by the manufacturer of the impedance analyzer is 300-800 × 10 9 /liter. In previous studies comparing impedance and flow cytometrybased platelet counts in cats, both comparatively higher and lower impedance-based counts have been reported.…”

Section: Discussionmentioning

confidence: 99%

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Assessment of platelet function in healthy sedated cats using three whole blood platelet function tests

Abrams‐Ogg

Wood

et al. 2015

J VET Diagn Invest

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Abstract. The objectives of this study were to establish feline references intervals for 3 commercial whole blood platelet function test analyzer systems: Multiplate analyzer (MP; Roche Diagnostics International Ltd., Rotkreuz, Switzerland), Platelet Function Analyzer-100 (PF: Siemens Canada, Mississauga, Ontario, Canada), and Plateletworks Combo-25 kit (PW; Helena Laboratories, Beaumont, TX). Venipuncture was performed on 55 healthy sedated cats, and platelet aggregation in response to adenosine diphosphate (ADP), collagen (COL), and arachidonic acid (AA; MP only) was assessed using citrated blood. For the MP analyzer, median (95% confidence intervals [CIs]) area under curve (Units) for ADP, COL, and AA agonists were 87 (11-176), 81 (32-129), and 91 (59-129), respectively. For the PF analyzer, median (95% CIs) closure time, using COL-ADP cartridges, was 69 (46-89) sec. For the PW assay, median (95% CIs) percent aggregations for ADP and COL agonists were 71 (18-92) and 49 (9-96), respectively, using impedance hematology analyzer platelet counts, and 94 (25-98) and 68 (14-119), respectively, using flow cytometry hematology analyzer platelet counts. There were low correlations between the PF analyzer (COL-ADP cartridge) and MP analyzer (COL agonist; ρ = 0.11), and between the PF analyzer (COL-ADP cartridge) and PW assay (COL agonist using impedance platelet counts; ρ = 0.14). The PW assay percent aggregations using impedance and flow cytometric platelet counts were correlated for both ADP (ρ = 0.64) and COL (ρ = 0.64) agonists. Platelet function testing using these tests are feasible in cats, but 95% CIs are wide, so single results may be difficult to interpret. Platelet counting by impedance or flow cytometry may be used for the PW assay but are not interchangeable.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Assessment of platelet function in healthy sedated cats using three whole blood platelet function tests

Abrams‐Ogg

Wood

et al. 2015

J VET Diagn Invest

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“…Dewhurst et al (2003), observaram excelente correlacao do VG determinado por impedância utilizando dois aparelhos comerciais em amostras de cães e gatos. Becker et al (2008), comparando aparelhos eletrônicos com diferentes tecnologias, obtiveram uma excelente correlação entre os metodos em amostras de cães e gatos.…”

Section: Discussionunclassified

Uso Da Impedância Na Determinação Do Volume Globular Das Espécies Felina, Equina E Bovina

Vieira¹,

Viol²,

Barbosa³

et al. 2008

AVS

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Endereço para correspondência: Paulo Cesar Cialini -ciarlini@fmva.unesp.br R RE ES SU UM MO OObjetivou-se determinar a correlação entre os valores de volume globular (VG) das espécies felina, equina e bovina com dois diferentes métodos: por impedância e pelo método de Strumia. Para tal, amostras de sangue de 70 gatos, 80 cavalos e 77 bois foram analisadas. Os valores de VG, em ambos os métodos, foram obtidos num período compreendido entre 20 -120 minutos após a colheita. Os valores de VG obtidos pelos métodos de Strumia e impedância apresentaram correlação (r = 0,98 gatos, r = 0,98 cavalos e r = 0,96 bois) extremamente significante (p < 0,001) para as três espécies. Concluiu-se que o valor do VG determinado por impedância revelou-se confiável e é recomendado para o uso clínico nas espécies felina, equina e bovina. Palavras-chave: hematócrito, Strumia, volume globular, impedância A AB BS ST TR RA AC CT TThe aim of this study was to determinate the correlation of packed cell volume (PCV) between cats, horses and cows with two different methods: by impedance and by the Strumia method. For this purpose, blood samples from 70 cats, 80 horses and 77 cows were analyzed. The PCV data, in both methods, were determined at 20 -120 minutes after blood collection. The PCV data obtained by Strumia and impedance methods (r = 0.98 cats, r = 0.98 horses and r = 0.96 cows) were extremely significant (p < 0.0001) for the three species. In conclusion, the PCV data determined by impedance was reliable and it is recommended for clinical use in feline, equine and bovine species.

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“…The manual counts are affected by human counting errors (Becker et al, 2008). Thus, comparing the ACI results with the MC might bring more uncertainty to the comparison process.…”

Section: Discussionmentioning

confidence: 99%

“…This is mainly due to the variety of animal species and the difficulty in finding an economically viable technical approach capable of handling all variations (e.g., concentration, size and shape) in those blood samples. Additionally, the tests carried out in electrical impedance based equipments require the use of specific reagents for each animal species (Becker et al, 2008). This feature leads to the lack of reagent for some species and the need for the laboratory to have many reagents even when few tests are needed for a given species.…”

Section: Introductionmentioning

confidence: 99%

Image-based red blood cell counter for multiple species of wild and domestic animals

Maurício

Schneider

Takahira

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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RBC count plays an important role in animal diagnosis. Despite the many technologies available in different automated hematology analyzers, when it comes to the blood of wild animals it is still difficult to find an easy and affordable solution for multiple species. This study aims to evaluate the proposed automatic red blood cell counter. Blood samples (1 ocelot -Leopardus pardalis, 1 monkey -Cebus apella, 1 coati -Nasua nasua, 62 dogs -Canis familiaris, and 5 horses -Equus caballus) were analyzed using three methods: 1-manual count, 2-automatic count by image, and 3-semi-automatic count by image; blood from dogs and horses were also analyzed by a fourth method: 4-automatic count by impedance. The counts in methods 2 and 3 were produced by the proposed red blood cell counter. Results were compared using Pearson's correlation and plots with different methods as the criterion standard. RBC counts in methods 1, 2, and 3 correlated very well with those in the method 4 (r ≥ 0.94). RBC counts produced by method 2 were highly correlated with method 3 (r = 0.998). The results indicate that the proposed method can be used as an automatic or semi-automatic counting method in clinics that are currently using the manual method for RBC assessment.

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Comparative clinical study of canine and feline total blood cell count results with seven in‐clinic and two commercial laboratory hematology analyzers

Cited by 55 publications

References 17 publications

Assessment of platelet function in healthy sedated cats using three whole blood platelet function tests

Assessment of platelet function in healthy sedated cats using three whole blood platelet function tests

Uso Da Impedância Na Determinação Do Volume Globular Das Espécies Felina, Equina E Bovina

Image-based red blood cell counter for multiple species of wild and domestic animals

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