Comparative characterization of Corynebacterium pseudotuberculosis from goats in Kerala, India and reference strain

Vathsala, M.; Jayaprakasan, V.

doi:10.1016/j.smallrumres.2007.03.004

Cited by 10 publications

(3 citation statements)

References 9 publications

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“…The results of the susceptibility tests confirm that the reference strains of C. pseudotuberculosis are sensitive to conventional antibiotics ( 23 , 31 , 32 ). Some studies show that different species of Corynebacterium may be resistant to antibiotics as a consequence of the presence of genes located on plasmids that confer resistance to streptomycin, erythromycin, chloramphenicol, and tetracycline ( 33 – 35 ) and also to a resistance profile related to the activity beta-lactamases ( 36 ).…”

Section: Discussionsupporting

confidence: 56%

Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

et al. 2021

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Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis in small ruminants, a chronic disease characterized by the development of granulomas in superficial and visceral lymph nodes as well as in several organs. An important characteristic of the infection with this bacterium is the formation of a biofilm and the absence of effective antibiotic therapy against the disease. From this scenario, the objective of this study was to evaluate the susceptibility of C. pseudotuberculosis to conventional antibiotics and to red, green, and brown propolis extracts obtained by the supercritical and ethanolic extraction methods as well as its activity in the bacterial biofilm. The results of the sensitivity test using antibiotics indicated a sensitivity of C. pseudotuberculosis strains to the antimicrobial agents. The ethanolic extract of green propolis and the supercritical red propolis extract showed the best antibacterial activities against planktonic C. pseudotuberculosis. A lower antimicrobial activity of the brown propolis extract was identified. Propolis extracts were effective in interfering with the formation of the C. pseudotuberculosis biofilm but had little activity on the consolidated biofilm. In conclusion, propolis extracts are more effective against C. pseudotuberculosis in the planktonic stage, being able to interfere with the formation of bacterial biofilm. However, the action of propolis extracts in a sessile and structured microbial biofilm is reduced.

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Section: Discussionsupporting

confidence: 56%

Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

et al. 2021

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“…[34], and Mohan et al . [35]. Table-1 indicates that vancomycin (100%), ciprofloxacin (100%), amikacin (100%), neomycin (100%), gentamicin (100%), streptomycin (100%), novobiocin (100%), trimethoprim/sulfamethoxazole (96%), rifampicin (86%), penicillin G (65%), and TE (78%) were sensitive to Staphylococcus isolates.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial efficacy of Moringa oleifera leaf extract against pyogenic bacteria isolated from a dromedary camel (Camelus dromedarius) abscess

2019

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Background: Abscess in camel is one of the most important bacterial infections. It causes anemia and emaciation, resulting in an economic loss due to carcass condemnation and a decrease in reproductive and production efficiency. Aim: This investigation aimed to isolate the bacteria from abscesses in camels and evaluate the antibacterial activity of Moringa oleifera extracts. Materials and Methods: Disk diffusion method and minimum inhibitory concentration were used for the evaluation of the antibacterial activity of M. oleifera extracts against isolated bacteria from camel abscesses. Results: The isolated bacteria were displayed relatively as follows: Corynebacterium pseudotuberculosis (30.4%), Staphylococcus aureus (25.8%), Escherichia coli (17.8%), Corynebacterium ulcerans (10.5%), Klebsiella pneumoniae (8.5%), Pseudomonas aeruginosa (8.5%), Micrococcus spp. (6.7%), Proteus vulgaris (5.2%), Citrobacter spp. (4.2%), and Staphylococcus epidermidis (1.7%). The drugs of choice for Corynebacterium isolates were ciprofloxacin and trimethoprim/ sulfamethoxazole, whereas amikacin, ciprofloxacin, gentamicin, neomycin, novobiocin, streptomycin, and vancomycin were for Staphylococcus isolates. Moreover, the ethanol extracts of M. oleifera showed higher antibacterial efficacy than the cold aqueous extracts. Conclusion: M. oleifera is considered one of the new infection-fighting strategies in controlling pyogenic bacteria responsible for camel abscesses.

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“…Although the disease can be diagnosed through microbiological culture of caseous material, most infected animals may develop internal granulomas, commonly in the lungs or mediastinal lymph nodes, not expressing clinical signs of infection (BINNS et al, 2007;LIMA et al, 2017). Once established in the herd, the eradication of this disease is a difficult step, due to the difficulty in treatment and the detection of animals contaminated by clinical signs has its efficiency limited (MOHAN et al, 2008;PAULE et al, 2004;SÁ et al, 2021). Given this complexity, new biotechnological approaches have been used by researchers to unravel the molecular mechanisms of virulence of C. pseudotuberculosis, aiming to mimic in vitro the in vivo conditions in which the agent installs itself and develops the infectious picture.…”

Section: Introductionmentioning

confidence: 99%

Influence of Iron Chelating Agents on the in Vitro Growth Curve of Corynebacterium pseudotuberculosis Strains

et al. 2022

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Corynebacterium pseudotuberculosis é o agente causador da linfadenite caseosa dos caprinos e ovinos, enfermidade associada a perdas econômicas diretas no setor pecuário. Além de ter um curso crônico, o seu tratamento com antibióticos é inviável e não existem vacinas eficazes disponíveis no mercado. Neste experimento, foram utilizadas condições de cultivo in vitro que se aproximassem do ambiente das condições in vivo encontradas pela bactéria durante a infecção do hospedeiro, isto incluiu a utilização de material biológico animal como meio de cultura e a restrição do ferro livre no meio. Nessas condições, era esperado que o C. pseudotuberculosis expressasse perfis proteicos de membrana ainda desconhecidos. Par isso foram utilizadas linhagens bacterianas de baixa (T1) e alta (VD57) virulência, cultivadas em caldo BHI, MQD, MQD suplementado com ferro, RPMI, RPMI suplementado com soro ovino e um meio produzido com material biológico ovino, todos estes com e sem suplementação de agentes quelantes de ferro. As massas bacterianas foram coletadas durante as fases Log e Estacionária da curva de crescimento da bactéria, para que fosse conduzida a extração das moléculas de membrana com o uso de solventes orgânicos. Este procedimento gerou uma fração denominada antígenos de membrana, rica em glicoproteínas. O perfil proteico variou de acordo com o meio utilizado, sendo que algumas bandas coradas na eletroforese aparecem em muitos meios. Portanto foi possível produzir informações importantes para uma futura vacina e produção testes diagnósticos. Palavras-chave: C. pseudotuberculosis. Meios de Cultura. Quelantes de Ferro. Proteínas de Membrana Bacteriana. Abstract Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis in goats and sheep, a disease associated with direct economic losses in the livestock sector. In addition to having a chronic course, its treatment with antibiotics is unfeasible and there are no effective vaccines available on the market. In this experiment, in vitro culture conditions that approximated the environment to the in vivo conditions found by the bacterium during host infection were used, this included the use of animal biological material as a culture medium and the restriction of free iron in the medium. Under these conditions, C. pseudotuberculosis was expected to express unknown membrane protein profiles yet. Also, bacterial strains of low (T1) and high (VD57) virulence were used, grown in BHI broth, MQD, MQD supplemented with iron, RPMI, RPMI supplemented with sheep blood serum and a culture medium produced with biological material, all of these with and without supplementation of iron chelating agents. Bacterial masses were collected during the Log and Stationary phases of the bacterial growth curve, so that membrane molecules could be extracted using organic solvents. This procedure generated a fraction called membrane antigens, rich in glycoproteins, with a protein profile varying among the different growth media tested, demonstrating the importance of the medium for bacterial protein expression and consequently influencing the expressed protein profile for test production diagnoses and a future vaccine. Keywords: C. pseudotuberculosis. Culture Medium. Iron Chelators. Bacterial Membrane Proteins.

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Comparative characterization of Corynebacterium pseudotuberculosis from goats in Kerala, India and reference strain

Cited by 10 publications

References 9 publications

Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

Antibacterial efficacy of Moringa oleifera leaf extract against pyogenic bacteria isolated from a dromedary camel (Camelus dromedarius) abscess

Influence of Iron Chelating Agents on the in Vitro Growth Curve of Corynebacterium pseudotuberculosis Strains

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