Comparative capillary chromatographic and capillary electrochromatographic enantioseparations using cellulose tris(3,5-dichlorophenylcarbamate) as chiral stationary phase

Chankvetadze, Bezhan; Kartozia, Irma; Breitkreutz, Jörg; Girod, Marco; Knobloch, Martin; Okamoto, Yoshio; Blaschke, Gottfried

doi:10.1002/1615-9314(20010401)24:4<251::aid-jssc251>3.0.co;2-t

Cited by 48 publications

(34 citation statements)

References 21 publications

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“…The advantage of wide-pore materials has been recently shown by Låmmerhofer et al [33] for monolithoic type organic polymers containing tert-butylcarbamoylquinine as chiral selector. The favorable effect of wide pores on the peak efficiency in enantioseparations has been shown in comprehensive studies performed on polysaccharide derivatives coated on silica gels of different pore size [34,63,117,121]. It is obvious from the results shown in Fig.…”

Section: Pore Size Of the Supportmentioning

confidence: 73%

“…In this case, an increase of the ionic strength of the buffer may allow, due to a reduction of the double electric layer thickness, to avoid their overlapping and subsequently, the EOF may increase with increasing buffer concentrations (Fig. 6) [121].…”

Section: Mobile Phasementioning

confidence: 99%

“…Opposite to the use of longer packed beds the current trend in chiral CEC is to shorten the packed bed and this way the analysis time. This approach has been successfully used by several groups [33,83,121]. It must be noted that only chiral selectors with high chiral recognition ability will be applicable for the enantioseparations in short capillaries.…”

Section: Other Variablesmentioning

confidence: 99%

“…More comprehensive studies in the wide range of flow rates indicated that CEC offers the most distinct advantages in a higher linear flow-rate range, e.g., in the case of fast analysis which is a big demand in separation science at present (Fig. 13) [117]. Before a wide implementation of CEC for practical problems the practitioners will put the advantages which this technique offers against the demands which it implies to the mobile phase (electrical conductivity, low Joule heat generation, etc.)…”

Section: Cec Vs Hplcmentioning

confidence: 99%

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Enantioseparations by capillary electrochromatography

et al. 2001

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The review summarizes recent developments in enantioseparations by capillary electrochromatography (CEC). Selected fundamental aspects of CEC are discussed in order to stress those features which may allow the success of this technique in the competitive field of enantioseparations. In addition, the comparative characteristics of the different modes of chiral CEC and the stationary phases are presented. The effects of the characteristics of the stationary and liquid phases and operational conditions on the separation results are discussed. Finally, some future trends are briefly addressed.

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Section: Pore Size Of the Supportmentioning

confidence: 73%

Section: Mobile Phasementioning

confidence: 99%

Section: Other Variablesmentioning

confidence: 99%

Section: Cec Vs Hplcmentioning

confidence: 99%

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Enantioseparations by capillary electrochromatography

et al. 2001

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“…All of the above investigations were performed under aqueous mobile phases. There were only a few reports dealing with CEC enantioseparations under nonaqueous conditions [8][9][10] and separation of enantiomers based on cellulose derivative CSPs in nonaqueous phases has only been reported by Blaschke et al [11][12][13][14][15], according to our knowledge. Their experiments, however, were concentrated on the coated-type phases.…”

Section: Introductionmentioning

confidence: 94%

Separation of enantiomers by nanoliquid chromatography and capillary electrochromatography using a bonded cellulose trisphenylcarbamate stationary phase

Chen

Zou

et al. 2002

Electrophoresis

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A cellulose trisphenylcarbamate-bonded chiral stationary phase was applied to nano-liquid chromatography (nano-LC) and capillary electrochromatography (CEC) with nonaqueous and aqueous solutions as the mobile phases. Several chiral compounds were successfully resolved on the prepared phase by nano-LC. The applicability of nonaqueous CEC on a cellulose derivative stationary phase was investigated with the organic solvents methanol, hexane, 2-propanol, and tetrahydrofuran (THF) containing acetic acid, as well as triethylamine as the mobile phases. Enantiomers of warfarin and praziquantel were baseline-resolved with plate numbers of 82,300 and 38,800 plates/m, respectively, for the first eluting enantiomer. The influence of applied voltage, concentration of nonpolar solvent, apparent pH, and buffer concentration in the mobile phase on the electroosmotic flow (EOF) and the mobility of the enantiomers was evaluated. Enantioseparations of trans-stilbene oxide and praziquantel were also achieved in aqueous CEC with plate numbers of 111,100 and 107,400 plates/m, respectively, for the first eluting enantiomer. A comparison between nonaqueous CEC and aqueous CEC based on a cellulose trisphenylcarbamate stationary phase was discussed. Pressure-assisted CEC was examined for the chiral separation of praziquantel and faster analysis with high enantioselectivity was acquired with the proper pressurization of the inlet vial.

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Effect of organic solvent, electrolyte salt and a loading of cellulose tris (3,5-dichlorophenyl- carbamate) on silica gel on enantioseparation characteristics in capillary electrochromatography

Chankvetadze¹,

Kartozia²,

Breitkreutz

et al. 2001

Electrophoresis

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The effect of the amount of the chiral selector, cellulose tris(3,5-dichlorophenylcarbamate) (CDCPC) on the separation characteristics of enantiomers of some charged and neutral analytes was studied in capillary electrochromatography (CEC). For better understanding of the effect of the loading of the chiral selector on the particles and pore size of the packing material, laser-beam particle size analyzer and scanning electron microscopy (SEM) were used. As shown in this study, CDCPC can be used for CEC enantioseparations of a wide range of chiral charged and neutral analytes with high efficiency. The loading of the polysaccharide derivative on the surface of silica materials even in high amounts does not markedly affect the particle size and porous structure of the packing material. The separation characteristics are strongly affected by the loading of CDCPC onto silica gel in both CEC and capillary liquid chromatography (CLC).

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Comparative capillary chromatographic and capillary electrochromatographic enantioseparations using cellulose tris(3,5-dichlorophenylcarbamate) as chiral stationary phase

Cited by 48 publications

References 21 publications

Enantioseparations by capillary electrochromatography

Enantioseparations by capillary electrochromatography

Separation of enantiomers by nanoliquid chromatography and capillary electrochromatography using a bonded cellulose trisphenylcarbamate stationary phase

Effect of organic solvent, electrolyte salt and a loading of cellulose tris (3,5-dichlorophenyl- carbamate) on silica gel on enantioseparation characteristics in capillary electrochromatography

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