Enantioseparations by capillary electrochromatography

Fanali, Salvatore; Catarcini, Paolo; Blaschke, Gottfried; Chankvetadze, Bezhan

doi:10.1002/1522-2683(200109)22:15<3131::aid-elps3131>3.0.co;2-s

Cited by 150 publications

(57 citation statements)

References 117 publications

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“…In spite of the drawbacks of CEC compared with CE in chiral analysis (CEC is less flexible and enables to achieve lower peak efficiency than CE [50]), CEC is entering in the field of practical applications [35,36,[51][52][53][54].…”

Section: Cecmentioning

confidence: 99%

Sensitive chiral analysis by capillary electrophoresis

García-Ruiz

Marina

2006

Electrophoresis

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Sensitive chiral analysis by capillary electrophoresisIn this review, an updated view of the different strategies used up to now to enhance the sensitivity of detection in chiral analysis by CE will be provided to the readers. With this aim, it will include a brief description of the fundamentals and most of the recent applications performed in sensitive chiral analysis by CE using offline and online sample treatment techniques (SPE, liquid-liquid extraction, microdialysis, etc.), on-column preconcentration techniques based on electrophoretic principles (ITP, stacking, and sweeping), and alternative detection systems (spectroscopic, spectrometric, and electrochemical) to the widely used UV-Vis absorption detection.

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Section: Cecmentioning

confidence: 99%

Sensitive chiral analysis by capillary electrophoresis

García-Ruiz

Marina

2006

Electrophoresis

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“…Recent results focusing on the improvement of column technology expanded the selectivity of this miniaturized technique, that at present is able to perform rapid separations of many classes of compounds also including charged species such as chiral and nonchiral drugs, metabolites, food additives, pesticides, protein digest, peptides, etc. [4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

CEC separation of insect oostatic peptides using a strong‐cation‐exchange stationary phase

Rocco¹,

Aturki²,

D’Orazio³

et al. 2007

Electrophoresis

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The separation of several insect oostatic peptides (IOPs) was achieved by using CEC with a strong-cation-exchange (SCX) stationary phase in the fused-silica capillary column of 75 mm id. The effect of organic modifier, ionic strength, buffer pH, applied voltage, and temperature on peptides' resolution was evaluated. Baseline separation of the studied IOPs was achieved using a mobile phase containing 100 mM pH 2.3 sodium phosphate buffer/ water/ACN (10:20:70 v/v/v). In order to reduce the analysis time, experiments were performed in the short side mode where the stationary phase was packed for 7 cm only. The selection of the experimental parameters strongly influenced the retention time, resolution, and retention factor. An acidic pH was selected in order to positively charge the analyzed peptides, the pI's of which are about 3 in water buffer solutions. A good selectivity and resolution was achieved at pH ,2.8; at higher pH the three parameters decreased due to reduced or even zero charge of peptides. The increase in the ionic strength of the buffer present in the mobile phase caused a decrease in retention factor for all the studied compounds due to the decreased interaction between analytes and stationary phase. Raising the ACN concentration in the mobile phase in the range 40-80% v/v caused an increase in both retention factor, retention time, and resolution due to the hydrophilic interactions of IOPs with free silanols and sulfonic groups of the stationary phase.

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“…In recent years, enantioseparations by CEC have received much attention as a potential technique in the analysis of enantiomers [1][2][3][4][5][6]. Polysaccharide derivative-based chiral stationary phases (CSPs) have been shown the efficient chiral recognition ability for a variety of racemic compounds in HPLC and have been extensively studied [7,8].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrochromatographic separation of enantiomers on chemically bonded type of cellulose derivative chiral stationary phases with a positively charged spacer

et al. 2003

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Capillary electrochromatographic separation of enantiomers on chemically bonded type of cellulose derivative chiral stationary phases with a positively charged spacer Positively charged chiral stationary phases (CSPs) were prepared for capillary electrochromatography (CEC) separation of enantiomers by chemically immobilizing cellulose derivatives onto diethylenetriaminopropylated silica (DEAPS) with tolylene-2,4-diisocyanate (TDI) as a spacer reagent. Anodic electroosmotic mobility was observed in both nonaqueous and aqueous mobile phases due to the positively charged amines on the surface of the prepared CSPs. For comparison, the traditionally used 3-aminopropyl silica (APS) was also adopted as the base material instead of DEAPS to prepare CSP. It was observed that the EOF on the DEAPS-based CSP was 18%-60% higher than that on the APS-based CSP under nonaqueous mobile phase conditions. Separation of enantiomers in CEC was performed on the positively charged CSPs with the nonaqueous mobile phases of pure ethanol or mixture of hexane-alcohol and the aqueous phases of acetonitrile-water or 95% ethanol. Fast separation of enantiomers was achieved on the newly prepared CSPs.

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Enantioseparations by capillary electrochromatography

Cited by 150 publications

References 117 publications

Sensitive chiral analysis by capillary electrophoresis

Sensitive chiral analysis by capillary electrophoresis

CEC separation of insect oostatic peptides using a strong‐cation‐exchange stationary phase

Capillary electrochromatographic separation of enantiomers on chemically bonded type of cellulose derivative chiral stationary phases with a positively charged spacer

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