Comparative behaviour of L-929 fibroblastic and human endothelial cells onto crosslinked protein substrates

Abstract

Studies were carried out to compare the behaviour of human umbilical vein endothelial cells (HUVEC) and L-929 fibroblastic cells towards proteins crosslinked by glutaraldehyde (GTA) or carbodiimide (CDI) proposed for coating of vascular prostheses. CDI crosslinking of bovine serum albumin used alone, or mixed with gelatin, allowed higher rates of cell growth and DNA synthesis than GTA crosslinking independent of cells. Assessment of the plating efficiency revealed a similar behaviour of both cells towards membranes and reference plastic surface in terms of percentages of bound cells. HUVEC proliferation onto CDI crosslinked gelatin and/or albumin membranes did not differ significantly whereas the growth of L-929 was enhanced onto gelatin albumin membranes in comparison with both gelatin membranes and the reference surface. The analysis of DNA synthesis corroborated the results of the growth curves and elicited a delay of the growth phases in HUVEC cultured onto CDI crosslinked membranes, unlike the L-929 fibroblast.

Heparin immobilized on proteins usable for arterial prosthesis coating: Growth inhibition of smooth-muscle cells

“…22 SMC growth on gelatin and/or albumin gels did not differ significantly and was even better than on tissue culture plastic. Previous work 23 reported similar tendencies of human umbilical vein endothelial cells and of L-929 fibroblasts grown on EDAC-crosslinked gels. However, bovine SMC proliferated more rapidly than bovine endothelial cells.…”

Electrospun poly(ɛ-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering

“…Using such biocomposites, the synthetic polymeric materials can function as a fibrous backbone providing mechanical properties while natural polymer component can act as promoters of cellular attachment and growth. Reports by a few researchers demonstrated that gelatin enhances the attachment and proliferation of cells [15,[34][35][36][37][38][39]. However, there are no reports utilizing PCL/gelatin nanofibrous scaffolds for nerve tissue engineering.…”

Biodegradable Gelatin/beta-Tricalcium Phosphate Composite Microspheres As Localized Delivery of Growth Factor- Velvet Antler Polypeptides by Water-in-Oil (W/O) Emulsion Process

“…It has been extensively utilized for pharmaceutical and medical purpose, and its biosafety has been proven through the long clinical applications (20). A many of documents from some researches show the attachment and proliferation of cells on substrates are promoted by the surface coating of gelatin (21,22). Additionally, because of the different gelatin has different isoelectric point, gealtin can be chosen to bind the protein drugs through ionic complex (23).…”