Comparative assessment of the effectiveness of different cleaning methods on the growth of Candida albicans over acrylic surface

Gantait, Subhajit; Bhattacharyya, Jayanta; Das, Samiran; Biswas, Swati; Ghati, Amit; Ghosh, Soumitra; Goel, Preeti

doi:10.4103/0976-237x.188554

Cited by 15 publications

(6 citation statements)

References 21 publications

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“…Chlorhexidine is a broad-spectrum biocide that also displays long-lasting residual activity in comparison to other biocides (212). It is most active against Gram-positive bacteria but also possesses activity against Gramnegative bacteria, some enveloped viruses, and fungi (213)(214)(215). However, it shows poor activity against nonenveloped viruses, and chlorhexidine is inactive against bacterial spores (201).…”

Section: Chlorhexidinementioning

confidence: 99%

Current and Emerging Topical Antibacterials and Antiseptics: Agents, Action, and Resistance Patterns

2017

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Bacterial skin infections represent some of the most common infectious diseases globally. Prevention and treatment of skin infections can involve application of a topical antimicrobial, which may be an antibiotic (such as mupirocin or fusidic acid) or an antiseptic (such as chlorhexidine or alcohol). However, there is limited evidence to support the widespread prophylactic or therapeutic use of topical agents. Challenges involved in the use of topical antimicrobials include increasing rates of bacterial resistance, local hypersensitivity reactions (particularly to older agents, such as bacitracin), and concerns about the indiscriminate use of antiseptics potentially coselecting for antibiotic resistance. We review the evidence for the major clinical uses of topical antibiotics and antiseptics. In addition, we review the mechanisms of action of common topical agents and define the clinical and molecular epidemiology of antimicrobial resistance in these agents. Moreover, we review the potential use of newer and emerging agents, such as retapamulin and ebselen, and discuss the role of antiseptic agents in preventing bacterial skin infections. A comprehensive understanding of the clinical efficacy and drivers of resistance to topical agents will inform the optimal use of these agents to preserve their activity in the future.

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Section: Chlorhexidinementioning

confidence: 99%

Current and Emerging Topical Antibacterials and Antiseptics: Agents, Action, and Resistance Patterns

2017

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“…Immersion in chemical disinfectant or cleaning solutions has been suggested as an important auxiliary method for eliminating biofilm (Gantait et al, 2016). Among the solutions, sodium hypochlorite is widely used, as it is capable of performing efficient cleaning (Sousa-Porta et al, 2013; Arruda et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Anti-biofilm Action of Chenopodium ambrosioides Extract, Cytotoxic Potential and Effects on Acrylic Denture Surface

et al. 2019

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Considering the challenge to control Candida -associated denture stomatitis, the search for antifungal substances derived from natural sources has become a trend in the literature. In this study the following effects of Chenopodium ambrosioides extract (CAE) were investigated: action against biofilms of Candida albicans , its cytotoxic potential, and changes caused in acrylic resin. The CAE was characterized by High Performance Liquid Chromatography (HPLC). The susceptibility of C. albicans to CAE was investigated by Minimum Inhibitory Concentration and Minimum Fungicidal Concentration (MIC and MFC) tests. Acrylic resin disks were fabricated, and C. albicans biofilms were developed on these for 48 h. Afterward the disks were immersed for 10 min in: PBS (Negative Control); 1% Sodium Hypochlorite (1% SH, Positive Control) or CAE at MIC or 5xMIC. The biofilms were investigated relative to counts and metabolic activity. The cytotoxic potential in keratinocytes and fibroblasts was verified by MTT test. Change in color and roughness of the acrylic resin was analyzed after 28 days of immersion in CAE. The data were analyzed by the ANOVA considering a 5% level of significance. The main compounds detected by HPLC were kaempferol and quercetin. Both MIC and MFC obtained the value of 0.25 mg/mL. The MIC was sufficient to significantly reduce the counts and activity of the biofilm cells ( p < 0.0001), while 5xMIC resulted in almost complete eradication, similar to 1% SH. Keratinocytes and fibroblasts exposed to the MIC and 5xMIC presented cell viability similar to that of the Control Group ( p > 0.05). No important changes in acrylic resin color and roughness were detected, even after 28 days. It could be concluded that the immersion of acrylic resin in C. ambrosioides extract in its minimum inhibitory concentration was effective for the reduction of C. albicans biofilms without any evidence of cytotoxic effects or changes in roughness and color of this substrate.

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“…This result is relevant, because roughness is a crucial factor for the adhesion of microorganisms on the acrylic surface. Therefore, it is of the utmost importance that chemical solutions do not change this property, because rough surfaces could favor the formation of biofilms [7, 24, 34, 42, 43]. Among the solutions for chemical control of biofilm, the 1%SH, used in an immersion protocol for 10 minutes, was considered the most efficient [34, 47].…”

Section: Discussionmentioning

confidence: 99%

Effect of Terminalia catappa Linn. on Biofilms of Candida albicans and Candida glabrata and on Changes in Color and Roughness of Acrylic Resin

Gonçalves

Madeira

Diniz

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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This study aimed to investigate the effect of the n-butanol fraction of Terminalia catappa Linn., (FBuTC) on biofilm of Candida albicans and Candida glabrata, as well as changes in color and roughness of polymethyl methacrylate resin (PMMA). The susceptibility of C. albicans and C. glabrata to FBuTC was evaluated by means of the Minimum Inhibitory and Minimum Fungicidal Concentration (MIC and MFC). PMMA acrylic resin discs (N= 108) were fabricated. For the susceptibility tests, biofilms of C. albicans and C. glabrata were developed on discs for 48 h and immersed in phosphate-saline buffer solution (PBS), 1% sodium hypochlorite (SH 1%), or FBuTC at MIC, 5xMIC, or 10xMIC. For the color and roughness change tests, the discs were immersed in distilled water, SH 1%, or FBuTC in the concentrations of 0.25 mg/mL, 2.5 mg/mL, or 25.0 mg/mL. After 28 days of incubation, color change was evaluated by spectrophotometry and roughness, by using a profilometer. The biofilms were investigated by one-way ANOVA and, the color and roughness changes (two-way ANOVA and the Tukey test; α=0.05). For both MIC and MFC the value of 0.25 mg/mL of FBuTC was observed for the planktonic cells of C. albicans and C. glabrata. Exposure to FBuTC at 10xMIC had a significant effect on the biofilm of C. albicans, showing a reduction in cell counts when compared with PBS, (p=0.001). For the biofilm of C. glabrata, the MIC was sufficient for significantly reducing the cell count (p<0.001). No important changes in color and roughness of the acrylic resin were observed, even after 28 days, irrespective of the concentration of FBuTC used (p >0.05). It could be concluded that the immersion of acrylic resin for dental prosthesis in FBuTC was effective in reducing the biofilms of C. albicans and C. glabrata without evidence of change in roughness and color of this substrate.

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Comparative assessment of the effectiveness of different cleaning methods on the growth of Candida albicans over acrylic surface

Cited by 15 publications

References 21 publications

Current and Emerging Topical Antibacterials and Antiseptics: Agents, Action, and Resistance Patterns

Current and Emerging Topical Antibacterials and Antiseptics: Agents, Action, and Resistance Patterns

Anti-biofilm Action of Chenopodium ambrosioides Extract, Cytotoxic Potential and Effects on Acrylic Denture Surface

Effect of Terminalia catappa Linn. on Biofilms of Candida albicans and Candida glabrata and on Changes in Color and Roughness of Acrylic Resin

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