Comparative assessment of the detection of candidal antigens as a diagnostic tool

Matthews, Ruth

doi:10.1080/02681219680000021

Cited by 14 publications

(8 citation statements)

References 80 publications

(86 reference statements)

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“…In some invasive fungal diseases, such as aspergillosis, cryptococcosis, and histoplasmosis, the detection of circulating antigens is a useful approach to immunodiagnosis (9,10,15,19,36) and, in some cases, may also help the clinician to evaluate the clinical response to antifungal therapy. Some tests have been used to detect gp43 circulating antigen with partial success in PCM (12,13,14,16,17,21,22,30).…”

mentioning

confidence: 99%

Monitoring gp43 Antigenemia in Paracoccidioidomycosis Patients during Therapy

Silva¹,

Queiroz‐Telles

Colombo

et al. 2004

J Clin Microbiol

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Paracoccidioidomycosis (PCM) is a systemic fungal disease that is particularly important among individuals living and working in rural areas of endemicity in Latin America. Detection of anti-Paracoccidioides brasiliensis antibodies is of limited value due to false-negative results. Detection of P. brasiliensis-gp43 circulating antigen is a practical approach for a specific diagnosis of the disease. In a previous study we described an inhibition enzyme-linked immunosorbent assay able to detect the 43-kDa P. brasiliensis antigen in sera of 100% of patients with the acute form of PCM and in 95.31 and 100% of patients with the chronic multifocal and unifocal forms of PCM. To investigate its potential application for the follow-up of PCM patients during treatment, antigen levels were monitored at regular intervals for up 8 to 12 months in serum samples from 23 patients. The results showed that treatment with itraconazole resulted in decreasing levels of circulating gp43 that were correlated with the reduction of anti-gp43 antibodies. It was also observed that by the end of 12 months of treatment gp43 levels were <5 g/ml in all patients.

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mentioning

confidence: 99%

Monitoring gp43 Antigenemia in Paracoccidioidomycosis Patients during Therapy

Silva¹,

Queiroz‐Telles

Colombo

et al. 2004

J Clin Microbiol

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“…In some invasive fungal diseases, the detection of circulating antigen is a useful approach to the serodiagnosis (8,11,17,21), and this may also be an alternative tool for the diagnosis of PCM patients. Some researchers have previously tried to detect circulating antigen in PCM patients using polyvalent an-tigens or antibodies in different assays such as enzyme-linked immunosorbent assay (ELISA) competition (14), immunoradiometric assay (10), immunoelectrophoresis-immunodiffusion (15), counterimmunoelectrophoresis (32), passive hemagglutination inhibition (20), inverted linear immunoelectrophoresis (19), and immunoblotting (26).…”

mentioning

confidence: 99%

Detection of Circulating gp43 Antigen in Serum, Cerebrospinal Fluid, and Bronchoalveolar Lavage Fluid of Patients with Paracoccidioidomycosis

Silva¹,

Colombo

Blotta

et al. 2003

J Clin Microbiol

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Paracoccidioidomycosis (PCM) is an important systemic fungal disease, particularly among individuals living and working in rural areas of endemicity in Latin America, who, without antifungal therapy, may develop fatal acute or chronic infection. For such patients, the detection of antibody responses by immunodiffusion is of limited value due to false-negative results. In contrast, the detection of Paracoccidioides brasiliensis gp43 circulating antigen may represent a more practical approach to the rapid diagnosis of the disease. Accordingly, an inhibition enzyme-linked immunosorbent assay (inh-ELISA) was developed for the detection of a 43-kDa P. brasiliensis-specific epitope incorporating a species-specific murine monoclonal antibody. With sera from patients with acute and chronic forms of the disease (n ‫؍‬ 81), the overall sensitivity of the test was found to be 95.1%, while specificity was found to be 97.5% compared to that with normal human sera from blood donors

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“…Verschiedene Immunoassays zur Detektion von Candida-Antigenen oder Candida-Stoffwechselprodukten sind entwickelt und mit sehr wechselnder Sensitivität und Spezifität getestet worden. Sie lassen sich deshalb zur Diagnostik nicht empfehlen [31,40,56]. Ein einfacher Nachweis gelingt beim Schleimhautbefall durch Abstrich, KOH-Färbung und Mikroskopie.…”

Section: Candidaunclassified

Infektionen unter immunsuppressiver Therapie nach Organtransplantation

2004

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The risk to acquire opportunistic infections is clearly increased in patients receiving immunosuppressive therapeutic regimens following organ transplantation or during treatment of autoimmune disorders. The modulation of the immune system can alter the clinical symptoms and the course of infectious diseases, including diagnostic signs such as fever or pathological changes in radiographs or blood cell counts. However, a rapid diagnosis and start of treatment is essential in these patients. Thus, a correct interpretation of even mild symptoms in the initial phase of an infectious disease is essential for establishing a diagnosis and initiation of a therapy at an early stage. Therefore, it is necessary that the clinical hallmarks of these diseases are widely known and that physicians treating these patients cooperate closely with transplant centers.

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Comparative assessment of the detection of candidal antigens as a diagnostic tool

Cited by 14 publications

References 80 publications

Monitoring gp43 Antigenemia in Paracoccidioidomycosis Patients during Therapy

Monitoring gp43 Antigenemia in Paracoccidioidomycosis Patients during Therapy

Detection of Circulating gp43 Antigen in Serum, Cerebrospinal Fluid, and Bronchoalveolar Lavage Fluid of Patients with Paracoccidioidomycosis

Infektionen unter immunsuppressiver Therapie nach Organtransplantation

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