Comparative assessment of faecal microbial composition and metabonome of swine, farmers and human control

Abstract

Shiang chiet tan 1 , chun Wie chong 2,3 , ivan Kok Seng Yap 4 , Kwai Lin thong 5 & cindy Shuan Ju teh 6 ✉ the gastrointestinal tract of humans and swine consist of a wide range of bacteria which interact with hosts metabolism. Due to the differences in co-evolution and co-adaptation, a large fraction of the gut microbiome is host-specific. In this study, we evaluated the effect of close human-animal interaction to the faecal metagenome and metabonome of swine, farmer and human control. three distinct clusters were observed based on T-RFLP-derived faecal microbial composition. However, 16S-inferred faecal microbiota and metabolic profiles showed that only human control was significantly different from the swine (p < 0.05). The metabonome of farmers and human controls were highly similar. Notably, higher trimethylamine N-oxide (TMAO) and butyrate were detected in human control and swine, respectively. The relative abundance of TMAO was positively correlated with Prevotella copri. overall, we compared and established the relationship between the metabolites and microbiota composition of swine, farmers and human control. Based on the data obtained, we deduced that long term occupational exposure to swine and farm environment had affected the gut bacterial composition of farmers. Nonetheless, the effect was less prominent in the metabolite profiles, suggesting the gut bacteria expressed high functional plasticity and are therefore resilience to the level of community shift detected.

Host phylogeny and host ecology structure the mammalian gut microbiota at different taxonomic scales

“…Across our surveyed herbivores, the most abundant bacterial taxa in the gut microbiota were Ruminococcaceae, Rikenellaceae, Lachnospiraceae , and Prevotellaceae which represent core taxa previously found in the gut microbiotas of many ruminants and herbivores in general, including cervids and bovids [8, 51], equids [52], elephants [53], and giraffes [54]. Ruminococcaceae and Lachnospiraceae have also been found in the guts of folivorous primates [3] and in domestic pigs [55, 56]. Members of these bacterial families are responsible for digesting the cellulose, hemicellulose, lignin, and protein found in vegetation, and fermenting these into short-chain fatty acids (SCFAs) [57].…”

Influenza A H1N1 Induced Disturbance of the Respiratory and Fecal Microbiome of German Landrace Pigs – a Multi-Omics Characterization

“…The metabolome analysis focused on short-chain fatty acids (SCFAs). Using nuclear magnetic resonance (NMR) analysis, we quantified the amounts of SCFAs of fecal samples during the infection experiment (Table S4) and compared the results to those of our previous study on the healthy gastrointestinal microbiome ( 35 ) and other comparable studies ( 46 – 48 ).…”

Influence of pig farming on human Gut Microbiota: role of airborne microbial communities

“…12 In addition, a very recent study has hypothesized that occupational contact between workers and pigs might also result in a bacterial community shift in the human gut. 14 However, there is still a lack of knowledge as to if and how occupational microbial exposure is linked to changes in the human gut's microbiota.…”