Comparative assessment of bone regeneration by histometry and a histological scoring system / Evaluarea comparativă a regenerării osoase utilizând histometria și un scor de vindecare histologică

Lucaciu, Ondine; Gheban, Dan; Şoriţău, Olga; Băciuţ, Mihaela; Câmpian, Radu Septimiu; Băciuț, Grigore

doi:10.1515/rrlm-2015-0009

Cited by 5 publications

(3 citation statements)

References 36 publications

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“…A certified histologist who was blinded to the group distribution performed the histological analysis using a histological bone-healing scale [ 26 ]. This scale provides a score for 17 histological parameters ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Comparison of rhBMP-2 in Combination with Different Biomaterials for Regeneration in Rat Calvaria Critical-Size Defects

Uribe

Vásquez

Alister

et al. 2022

BioMed Research International

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Regeneration of critical bone defects requires the use of biomaterials. The incorporation of osteoinductive agents, such as bone morphogenetic proteins (BMPs), improves bone formation. This study aimed to compare the efficacy of rhBMP-2 in combination with different materials for bone regeneration in critical-sized rat calvarial defects. This was an experimental animal study using 30 rats. In each rat, two 5-mm critical-size defects were made in the calvaria (60 bone defects in total) using a trephine. All rats were randomized to one of the six groups: control (C), autograft + rhBMP-2 (A), absorbable collagen sponge + rhBMP-2 (ACS), β-tricalcium phosphate + rhBMP-2 (B-TCP), bovine xenograft + rhBMP-2 (B), and hydroxyapatite + rhBMP-2 (HA). The outcome was assessed after 4 and 8 weeks using histological description and the histological bone healing scale. Statistical analysis was performed using the Kruskal-Wallis and Mann–Whitney U tests, with a p -value set at 0.05. The average bone healing scores per group were as follows: C group, 12.5; A group, 26.5; ACS group, 18.8; B-TCP group, 26.2; HA group, 20.9; and B group, 20.9. The C group showed a significant difference between weeks 4 and 8 ( p = 0.032 ). Among the 4-week groups, the C group showed a significant difference compared to A ( p = 0.001 ), ACS ( p = 0.017 ), and B-TCP ( p = 0.005 ) groups. The 8-week experimental group did not show any significant differences between the groups. The 5-mm critical size defect in rat calvaria requires the use of bone biomaterials to heal at 4 and 8 weeks. rhBMP-2, as applied in this study, showed no difference in new bone formation when combined with bovine, B-TCP, or HA biomaterials.

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Section: Methodsmentioning

confidence: 99%

Comparison of rhBMP-2 in Combination with Different Biomaterials for Regeneration in Rat Calvaria Critical-Size Defects

Uribe

Vásquez

Alister

et al. 2022

BioMed Research International

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“…Assessment of bone regeneration was performed following a modification of the histological evaluation method from Lucaciu et al [ 31 ] adapted for our study. Histological examination of slices was accomplished on a Zeiss Axio Scope A.1 photomicroscope.…”

Section: Methodsmentioning

confidence: 99%

Human Bone Marrow Mesenchymal Stromal Cells Promote Bone Regeneration in a Xenogeneic Rabbit Model: A Preclinical Study

Blanco

Briñón

Benito-Garzón

et al. 2018

Stem Cells International

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Significant research efforts have been undertaken during the last decades to treat musculoskeletal disorders and improve patient's mobility and quality of life. The goal is the return of function as quickly and completely as possible. Cellular therapy has been increasingly employed in this setting. The design of this study was focused on cell-based alternatives. The present study aimed at investigating the bone regeneration capacity of xenogeneic human bone marrow-derived mesenchymal stromal cell (hMSC) implantation with tricalcium phosphate (TCP) granules in an immunocompetent rabbit model of critical-size bone defects at the femoral condyles. Two experimental groups, TCP and hMSC + TCP, were compared. Combination of TCP and hMSC did not affect cell viability or osteogenic differentiation. We also observed significantly higher bone regeneration in vivo in the hMSC + TCP group, which also displayed better TCP osteointegration. Also, evidence of hMSC contribution to a better TCP osteointegration was noticed. Finally, no inflammatory reaction was detected, besides the xenotransplantation of human cells into an immunocompetent recipient. In summary, hMSC combined with TCP granules is a potential combination for bone regeneration purposes that provides better preclinical results compared to TCP alone.

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“…At four different periods (2, 4, 6 and 12 weeks) histological study were performed, where the percentage of osteoid (newly formed bone), proportion of woven to lamellar bone, and number of osteoblast and osteoclast cells were measured. The percentage of new bone formation was determined and converted to a percentage of total area of bone defect which is 8mm 2 in this study (Jang et al, 2008) (Wang et al, 2017) and intensity or numbers of osteoblast and osteoclast were measured according to the following scores: 0 absent 1 present at periphery 2 present at center 3 present at center and periphery (Lucaciu et al, 2015)…”

Section: Histologic Examinationmentioning

confidence: 99%

Augmentation of Surgically Created Bony Defects Using Biphasic Calcium Phosphate with And without Platelet Rich Fibrin: An Experimental Study in Sheep

Alkafarani¹,

Baban²

2019

juod

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Back ground and objective: The reconstruction of large bony defects is one of great challenge in clinical research, various materials and techniques were used in bone augmentation. Recently, autologous platelet rich fibrin (PRF) which contain various growth factors accelerates tissue healing and promotes bone regeneration. Therefore, this study aimed to evaluate the effectiveness of adding PRF to biphasic calcium phosphate (BCP) on healing process of iliac bone defects in sheep.Materials and methods: six iliac bone defect 8mm in diameters and depth were created in each side of four sheep. The superior two defects were filled with blood as control group, the middle two defects were filled with BCP and the inferior two defects were filled with a mixture of BCP and PRF equally. The PRF was prepared by centrifuging sheep own blood at 2700rpm for 12minutes. One sheep was sacrificed at 2, 4, 6 and 12 postoperatively weeks, and twelve iliac bone block from each period were prepared histologically and the slides stained with masson trichrome, hematoxylin and eosin stains for examination of new bone formation, bone maturation and intensity of osteoblast and osteoclast over healing time periods.Results: The study showed that the new bone formation percentage and the intensity of osteoblast and osteoclast cells were significantly increased in each group with healing time (p = <0.05) and they were in BCP+PRF group was higher than in BCP group and much higher than in control group at all-time intervals. Besides, the woven and lamellar bone percentages over time periods were significantly decreased and increased, respectively. Importantly, the study revealed that the (BCP+PRF) group had statistically significant increase in the percentage of new bone formation and lamellar bone and compared to the BCP and control groups in all time intervals. Conclusion: Within the limitations of this experimental study, our results demonstrated that the addition of PRF to BCP increases the formation of new bone.

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Comparative assessment of bone regeneration by histometry and a histological scoring system / Evaluarea comparativă a regenerării osoase utilizând histometria și un scor de vindecare histologică

Cited by 5 publications

References 36 publications

Comparison of rhBMP-2 in Combination with Different Biomaterials for Regeneration in Rat Calvaria Critical-Size Defects

Comparison of rhBMP-2 in Combination with Different Biomaterials for Regeneration in Rat Calvaria Critical-Size Defects

Human Bone Marrow Mesenchymal Stromal Cells Promote Bone Regeneration in a Xenogeneic Rabbit Model: A Preclinical Study

Augmentation of Surgically Created Bony Defects Using Biphasic Calcium Phosphate with And without Platelet Rich Fibrin: An Experimental Study in Sheep

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