Comparative Antioxidant Potential of Different Extracts of <i>Celastrus paniculatus</i> Willd. Seed

Abstract

The objective of the present study was to evaluate the comparative antioxidant potential of methanol, ethyl acetate, pet ether and water extracts of Celastrus paniculatus seed. Antioxidant activity was evaluated by using total phenol and flavonoid content determination assays, total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazil (DPPH) free radical assay, Reducing power assessment, Nitric oxide (NO) scavenging assay and Cupric ion reducing capacity assay (CUPRAC method). The extracts showed moderate antioxidant activity in a dose dependent manner. The extracts were found to contain phenolics and flavonoid compounds. In DPPH radical scavenging assay, ethyl acetate extract had the lowest IC50 value (585.58µg/ml) compared to ascorbic acid. In nitric oxide scavenging assay IC50 value was found to be 122.99µg/ml, 320.54µg/ml, 601.81µg/ml and 206.37µg/ml respectively for the Water, Methanol, Ethyl Acetate and Pet Ether extracts compared to 6.83µg/ml which was the IC50 value for the reference ascorbic acid. The extracts also showed good reducing power. The results of the present study indicate that the extracts possesses significant antioxidant potential of which ethyl acetate extract is the most promising one and possess highest antioxidant potential.

Comparative in Vitro Antioxidant Activity of Ethyl Acetate and Ethanol Extracts of Callicarpa Macrophylla

“…As, higher the absorbance of the extracts more will be the antioxidant activity. Previous studies also reported that the phenolic and flavonoid contents are responsible for antioxidant activity [17][18]. A study reported in 2016, by Sharma and his coworkers showed that methanol extract of the stem and leaves of Callicarpa arborea has an IC 50 value of 53.65 and 47.20 at the concentrations of 100-500µg/ ml, respectively containing phenolic compounds [17].…”

“…Another study reported that Celastrus paniculatus seeds ethyl acetate extract was IC 50 value of 558.58 and 601.81 µg/ml respectively, while the ascorbic acid was IC 50 value of 11.24 and 6.83µg/ml by DPPH and nitric oxide scavenging assay. Many biological activities related to antioxidants are not evaluated yet, so this plant is a better option for those activities [18].…”

An extensive review on phytochemistry and pharmacological activities of Indian medicinal plant Celastrus paniculatus Willd

“…A brief summary of the phytochemistry of C. paniculatus is presented in Table 1, including different parts of plant, type of solvents, morphological activity, types of active constituents and medicinal uses (Gurumurthy et al, 2008; Kumar & Gupta, 2002; Mekari et al, 2009; Zohera et al, 2010).…”

“…An investigation into possible toxic effects was conducted with polar and semi-polar compounds isolated from the fractionated seed oils in animal models. Upon injecting the fractionated extracts of seeds temporary adverse effects including fatty liver, degeneration of hepatic cells T A B L E 1 Morphological activity, active constituents and medicinal uses of various parts of C. paniculatus (Gurumurthy, Krishna, Patil, & Babu, 2008;Kumar & Gupta, 2002;Mekari, Patil, & Abilash, 2009;Zohera, Habib, Imam, Mazumdar, & Rana, 2010) and impairment of proximal tubular cells in the kidney were reported in rats (Bidwai, Wangoo, & Sharma, 1990).…”

Protective Effect of Celastrus paniculatus Seed Extract against Lead Acetate Induced Nephrotoxicity in Wistar Rats

“…[1][2][3] As the seeds were reported to possess significant antioxidant property, their protective effect against lead acetate induced nephrotoxicity has been investigated in this study by estimating biochemical, oxidative stress and antioxidant markers, histological and immunohistochemistry of KIM 1 expression. 4 The kidney is one of the most highly differentiated organs in the body that modulates a variety of physiologic processes especially the removal of drug metabolites. As per the Global Burden of Disease Study (2010), kidney diseases ranked 18 th in the list of causes of the total number of deaths worldwide that stood 27 th in 1990.…”