Comparative antibacterial activity of commercial chitosan and chitosan extracted from Auricularia sp.

Chang, Angela Kae T.; Frias, Ronaldo R.; Alvarez, Lourdes V.; Bigol, Ursela G.; Guzman, John Paul Matthew D.

doi:10.1016/j.bcab.2018.11.016

Cited by 76 publications

(35 citation statements)

References 11 publications

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“…The release of zinc ions in the culture medium was not related to the size of the inhibition zone, but rather related to the reduction of microbial colonies in the liquid culture medium [61]. The inhibitory activity of ZnAl/CS, which was superior to that of ZnA1, could be attributed to activation of the inhibitory activity of CS (effective against both Gram-positive and Gram-negative bacteria [62,63]) associated with Zn 2+ ions through ZnAl-LDH. The antibacterial activity of CS depends on different parameters.…”

Section: Resultsmentioning

confidence: 99%

Optimization of ZnAl/Chitosan Supra-Nano Hybrid Preparation as Efficient Antibacterial Material

Gohi

Zeng

et al. 2019

IJMS

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The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations. In this study, an antimicrobial hybrid supra-nano material was prepared by the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide. Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability. The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid. The antibacterial tests revealed that the ZnAl/CS hybrid possessed an enhanced antimicrobial effect against both Escherichia coli (E. coli, MTCC 739) and Penicilliumcyclopium (P. cyclopium, AS 3.4513). Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L. After optimization, it was found that the antibacterial activity of ZnAl/CS was strengthened against E. coli as evidenced by a widening of the inhibition zone of about 41.6%. The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.

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Section: Resultsmentioning

confidence: 99%

Optimization of ZnAl/Chitosan Supra-Nano Hybrid Preparation as Efficient Antibacterial Material

Gohi

Zeng

et al. 2019

IJMS

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“…Paper disks containing different concentrations of GTE were placed on the surface of the agar. The plates were then incubated at 35 °C for 24 h. The zones of inhibition (ZOI), indicated by clear halos around the disks, were measured using a Vernier caliper [ 35 ]. Tryptone Soya Broth (TSB) was used as the negative control.…”

Section: Methodsmentioning

confidence: 99%

“…To determine the MBC, the wells used in the determination of the MIC were streaked onto freshly-prepared TSA plates. The lowest concentration that yielded no growth was the MBC [ 35 ]. Tryptone Soya Broth (TSB) was used as the negative control.…”

Section: Methodsmentioning

confidence: 99%

Green tea (Camellia sinensis) extract inhibits biofilm formation in acyl homoserine lactone-producing, antibiotic-resistant Morganella morganii isolated from Pasig River, Philippines

Guzman

Alas

Lucban

et al. 2020

Heliyon

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The drastic development of urban districts around the world has caused changes in the environment, specifically on metropolitan waterways such as the Pasig River in the Philippines. These significant changes resulted in diversity of microorganisms and their mechanisms employed such as antibiotic resistance and their communication system or quorum sensing (QS). In this study, four bacterial isolates from Pasig River, identified as Aeromonas salmonicida , Acinetobacter sp., Morganella morganii , and Citrobacter freundii , were observed to employ short-chain acyl homoserine lactone (AHL) as their signalling molecule based on in vitro assays using the biosensor strain Chromobacterium violaceum CV026. Furthermore, M. morganii isolate was shown to be resistant to chloramphenicol. This poses a significant threat not just to public health but also to the aquatic life present in the river. Thus, green tea ( Camellia sinensis ) extract was tested for its capability to inhibit in vitro biofilm formation in M. morganii , as well as the short-chain acyl homoserine lactone QS system using C. violaceum ATCC 12472. Results showed that the extract significantly ( p < 0.05) inhibited biofilm formation in M. morganii at as low as 62.5 μg/mL (31.55%). Increasing the concentration (500 μg/mL) did not significantly ( p > 0.05) enhance the activity (41.21%). Furthermore, the extract also inhibited pigmentation in C. violaceum ATCC 12472, suggesting QS inhibition. This study adds into record the production of short-chain AHLs by Aeromonas salmonicida , Acinetobacter sp., Morganella morganii , and Citrobacter freundii , as well as the potential of green tea extract as inhibitor of biofilm formation in antibiotic-resistant M. morganii possibly through QS inhibition.

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“…The media is prepared in Moller Hinton agar (MHA) for growing the bacterial strain, 9.5 grams of MH agar was taken in 250 ml of distilled water [13][14][15][16][17][18][19][20][21][22][23]. The media and the empty Petri dishes wereautoclaved at 121 0 c for about one hour to sterilize the media and Petri dishes.…”

Section: Antibacterial Assaymentioning

confidence: 99%

Antibacterial potential test for Scheonoplectus Triqueter (L) Palla

2020

Lett Appl NanoBioSci

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Our present investigation aims to test the antibacterial action of Scheonoplectus triqueter (L) palla against the gram negative bacteria i.e. Escherichia coli and gram positive bacteria i.e staphylococcus aureus. The crude extract is taken in ethanol which is then fractionated into a different fraction in n-hexane, ethyl acetate, di-chloro methane (DCM) and water.The fractions are then separately applied to E.coli and s.aureus bacterial strains. Ampicillin (10 micro gram), Clindamycin (2 microgram) and Kanamycin sulfate (30 microgram) are used as a positive control while di-methyl sulfoxide (DMSO) is used as negative control. The result of antibacterial activity of Scheonoplectus triqueter (L) palla revealed to us that n-hexane section of the crude extractdisplayed relatively highest activity for E.coli (gram negative bacteria) and ethyl acetate fraction displayed relatively high activity for S.aureus (gram positive bacteria).

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Comparative antibacterial activity of commercial chitosan and chitosan extracted from Auricularia sp.

Cited by 76 publications

References 11 publications

Optimization of ZnAl/Chitosan Supra-Nano Hybrid Preparation as Efficient Antibacterial Material

Optimization of ZnAl/Chitosan Supra-Nano Hybrid Preparation as Efficient Antibacterial Material

Green tea (Camellia sinensis) extract inhibits biofilm formation in acyl homoserine lactone-producing, antibiotic-resistant Morganella morganii isolated from Pasig River, Philippines

Antibacterial potential test for Scheonoplectus Triqueter (L) Palla

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