Comparative analysis of yak milk and bovine milk glycoprotein N/O-glycome by online HILIC-UV-ESI-MS/MS

Comparative Analysis of Free and Glycoconjugates Oligosaccharide Content in Milk from Different Species

“…In particular, human and bovine milk glycoprotein N -glycans have been shown to exert significant antipathogenic activity against Listeria monocytogenes , the causative agent of listeriosis, inhibiting its growth and adhesion to the surface of Caco-2 intestinal epithelial cells . In this study, we found that yak milk was richer in glycoconjugates than other milk species, thus confirming an early report describing higher content of sialylated glycoconjugates in yak milk compared to bovine milk . Therefore, yak milk could represent an important source of glycoconjugates in infant formula.…”

“…36 In this study, we found that yak milk was richer in glycoconjugates than other milk species, thus confirming an early report describing higher content of sialylated glycoconjugates in yak milk compared to bovine milk. 18 Therefore, yak milk could represent an important source of glycoconjugates in infant formula. The present study describes the systematic comparison of free oligosaccharides and glycoconjugates content in milk from different species using the procedure combining the phenol-H 2 SO 4 method with the resorcinol-HCl method.…”

“…Our laboratory previously conducted a comparative analysis of goat milk glycoprotein N / O -glycans at different lactation stages, identifying 34 N -glycans and 11 O -glycans in colostrum, 39 N -glycans and 12 O -glycans in transitional milk, and 35 N -glycans and 8 O -glycans in mature milk . A similar comparative analysis of mature yak milk and bovine glycoprotein N/O -glycans revealed 79 N -glycans and 5 O -glycans in yak milk, and 78 N -glycans and 7 O -glycans in bovine milk . Furthermore, a study that used the resorcinol colorimetric method with N -acetylneuraminic acid as standard found 1.18 ± 0.09 mM glycoconjugate sialic acid in colostrum and 0.29 ± 0.02 mM in mature milk .…”

“…17 comparative analysis of mature yak milk and bovine glycoprotein N/O-glycans revealed 79 N-glycans and 5 Oglycans in yak milk, and 78 N-glycans and 7 O-glycans in bovine milk. 18 Furthermore, a study that used the resorcinol colorimetric method with N-acetylneuraminic acid as standard found 1.18 ± 0.09 mM glycoconjugate sialic acid in colostrum and 0.29 ± 0.02 mM in mature milk. 11 So far, the oligosaccharide content of glycoconjugates in milk from different species has not been reported.…”