Comparative analysis of virulence factors &amp; biotypes of Gardnerella vaginalis isolated from the genital tract of women with &amp; without bacterial vaginosis

Kumari, Nisha; Antony, Beena; Jeppu, Udayalaxmi

doi:10.4103/ijmr.ijmr_1674_16

Cited by 4 publications

(1 citation statement)

References 25 publications

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“…The propensity of G. vaginalis , compared to other BV-associated bacteria, to form a biofilm that provides a scaffold to which other potentially pathogenic bacteria can attach 85 – 88 may be very important. While the presence or absence of G. vaginalis has importance, quantitative analysis is more important with both traditional cultivation-independent 89 as well as novel molecular-based techniques 56 .…”

Section: Discussionmentioning

confidence: 99%

Recent advances in cultivation-independent molecular-based techniques for the characterization of vaginal eubiosis and dysbiosis

Lamont

Munckhof

Luef

et al. 2020

Fac Rev

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“The bacterial vaginosis syndrome” has significant adverse effects for women and babies, including preterm birth and increased risk of acquisition of sexually transmitted infections and HIV. Currently, the gold standard for diagnosis is Gram stain microscopy of vaginal secretions, which is not readily available, is somewhat subjective, and does not differentiate between the likely different subtypes of vaginal dysbioses that may have different etiologies, microbiology, responses to antibiotics, and phenotypic outcomes. With new information from molecular-based, cultivation-independent studies, there is increasing interest in the use of molecular techniques for the diagnosis of bacterial vaginosis. We reviewed the current evidence on and the rationale behind the use of molecular techniques for the diagnosis of bacterial vaginosis. We found a number of commercially available molecular diagnostic tests, a few of which have US Food and Drug Administration (FDA) and/or Conformité Européenne in vitro diagnostic (CE-IVD) approval, and we have compared their performance with respect to sensitivities and specificities. Molecular-based tests have the advantage of objectivity, quantification, detection of fastidious organisms, and validity for self-obtained vaginal swabs. The performance of the molecular tests against standard microscopy is impressive, but further education of users on interpretation is needed. Bacterial vaginosis is the major cause of vaginal dysbiosis and should be recognized for the threat it is to women’s genital tract health. Quantitative assessment of microbial abundance, the diversity of other organisms present, specific primers for gene sequence regions, and clades and biovars of target microbes should be recognized and incorporated into future molecular diagnostic tests to better differentiate between vaginal eubiosis and dysbiosis.

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