Comparative analysis of the spindle and chromosome configurations of in vitro–matured oocytes from patients with endometriosis and from control subjects: a pilot study

Barcelos, Ionara Diniz Evangelista Santos; Vieira, Rodolpho Cruz; Ferreira, E.; Martins, Wellington P.; Ferriani, Rui Alberto; Navarro, Paula Andrea

doi:10.1016/j.fertnstert.2009.05.006

Cited by 56 publications

(62 citation statements)

References 32 publications

(40 reference statements)

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“…Recent studies demonstrated significant DNA damage and increased anomalies in the microtubules and chromosomes of oocytes incubated with PF from endometriosis patients (Mansour et al, 2009;Carbone et al, 2003), which were prevented by supplementation of the culture medium with the antioxidant L-carnitine, suggesting that impaired oocyte quality in endometriosis may be mediated by oxidative stress (Carbone et al, 2003). Although the data were obtained from frozen/thawed MII mouse oocytes and may not necessarily be extrapolated to human oocytes, they support our hypothesis that oxidative stress might be involved in the delay or impairment of meiosis I in oocytes of women with endometriosis (Barcelos et al, 2009), a possibility that requires more in-depth evaluation in future studies.…”

Section: Endometriosis and Oxidative Stresssupporting

confidence: 63%

Endometriosis and Infertility: The Role of Oxidative Stress

Barcelos¹,

Navarro²

2012

Endometriosis - Basic Concepts and Current Research Trends

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Section: Endometriosis and Oxidative Stresssupporting

confidence: 63%

Endometriosis and Infertility: The Role of Oxidative Stress

Barcelos¹,

Navarro²

2012

Endometriosis - Basic Concepts and Current Research Trends

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“…Second, immature oocytes (GV and MI) were matured in the absence of companion cumulus cells , a fact possibly related to a higher frequency of meiotic and spindle anomalies [23], irrespective of the original oocyte quality. Third, IVM itself is known to cause a significant increase in the occurrence of meiotic abnormalities, as previously described [24]. Last, the low proportion of immature oocytes obtained in stimulated cycles and the need to subject them to IVM before fixation for immunofluorescence limited the supply of in vitromatured oocytes for the current study, justifying the very low number of samples analyzed.…”

Section: Discussionmentioning

confidence: 84%

Spindle and chromosome configurations of in vitro-matured oocytes from polycystic ovary syndrome and ovulatory infertile women: a pilot study

Vieira

Barcelos

Ferreira

et al. 2010

J Assist Reprod Genet

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Purpose To evaluate the meiotic spindle and chromosomal distribution of in vitro-matured oocytes from infertile nonobese women with PCOS and male or tubal causes of infertility (controls), and to compare in vitro maturation (IVM) rates between groups. Methods Seventy four patients (26 with PCOS and 48 controls) undergoing stimulated cycles of oocyte retrieval for ICSI were selected prospectively. Thirteen PCOS patients and 27 controls had immature oocytes retrieved submitted to IVM. After IVM, oocytes showing extrusion of the first polar body were fixed and processed for evaluation of the meiotic spindle and chromosome distribution by immunofluorescence microscopy.Results There were no differences between PCOS and control groups with respect to IVM rates (50.0% and 42.9%, respectively) nor the percentage of meiotic abnormalities in metaphase II oocytes (35.3% and 25%, respectively). Conclusions In vitro-matured oocytes obtained from stimulated cycles of nonobese PCOS did not have an increased ratio of meiotic abnormalities.

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“…The present data suggest that meiotic abnormalities are not responsible for compromised oocyte quality in infertile PCOS patients submitted to ovarian controlled hyperstimulation for ART, regardless of the women's weights. Also, the high prevalence of meiotic anomalies in all three groups led to the hint that IVM itself might cause a significant increase in the occurrence of meiotic abnormalities, which has been demonstrated by other observers [28]. The spindle of the oocyte is a highly dynamic structure that is extremely sensitive to the action of various factors [29,30,31].…”

Section: Discussionmentioning

confidence: 79%

“…The nonsignificant difference in the groups of the present study may be caused by the inclusion criteria that excluded patients who had any adverse endocrine conditions, such as hyperandrogenism, insulin resistance or hyperprolactinemia. Third, as mentioned above, IVM itself may cause a dramatic increase in the occurrence of meiotic abnormalities [28]. The high proportion of meiotic anomalies caused by IVM itself may cover the adverse effect on meiotic spindles and chromosomal segregations which come from obesity.…”

Section: Discussionmentioning

confidence: 99%

Confocal Microscopic Analysis of the Spindle and Chromosome Configurations of in vitro-Matured Oocytes from Different Types of Polycystic Ovary Syndrome Patients

Zhu

Han

Cao³

et al. 2015

Gynecol Obstet Invest

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Objective: To evaluate the meiotic spindle and chromosome distribution of in vitro-matured oocytes from infertile nonobese or obese women with polycystic ovary syndrome (PCOS) and to compare in vitro maturation (IVM) rates between groups. Design: Prospective study. Setting: Hospital-based IVF center. Patients: A total of 99 patients (26 obese women with PCOS, 25 nonobese women with PCOS, and 48 controls) undergoing stimulated cycles for intracytoplasmic sperm injection had immature oocytes for IVM. Interventions: Immature oocytes (germinal vesicle and metaphase I stages) were collected from obese and nonobese PCOS patients and controlled infertile patients. The meiotic spindle and chromosome configurations in oocytes matured in vitro were studied by confocal microscopy, with fluorescent labeling techniques for visualization of both microtubules and chromosomes. Main Outcome Measures: Meiotic spindle and associated chromosome configurations. Results: There were no significant differences between the different types of PCOS and the control group with respect to IVM rates (61.8, 63.8, and 63.2%, respectively), the percentage of spindle abnormalities in metaphase II oocytes (40.6, 42.9, and 37.5%, respectively) or chromosome abnormalities in metaphase II oocytes (31.2, 34.3, and 33.3%, respectively). Conclusions: In vitro-matured oocytes obtained from stimulated cycles had a high ratio of meiotic abnormalities. The different types of PCOS had the same ratio of meiotic abnormalities.

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Comparative analysis of the spindle and chromosome configurations of in vitro–matured oocytes from patients with endometriosis and from control subjects: a pilot study

Cited by 56 publications

References 32 publications

Endometriosis and Infertility: The Role of Oxidative Stress

Endometriosis and Infertility: The Role of Oxidative Stress

Spindle and chromosome configurations of in vitro-matured oocytes from polycystic ovary syndrome and ovulatory infertile women: a pilot study

Confocal Microscopic Analysis of the Spindle and Chromosome Configurations of in vitro-Matured Oocytes from Different Types of Polycystic Ovary Syndrome Patients

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