Spindle and chromosome configurations of in vitro-matured oocytes from polycystic ovary syndrome and ovulatory infertile women: a pilot study

Abstract

Purpose To evaluate the meiotic spindle and chromosomal distribution of in vitro-matured oocytes from infertile nonobese women with PCOS and male or tubal causes of infertility (controls), and to compare in vitro maturation (IVM) rates between groups. Methods Seventy four patients (26 with PCOS and 48 controls) undergoing stimulated cycles of oocyte retrieval for ICSI were selected prospectively. Thirteen PCOS patients and 27 controls had immature oocytes retrieved submitted to IVM. After IVM, oocytes showing extrusion of the first polar body were fixed and processed for evaluation of the meiotic spindle and chromosome distribution by immunofluorescence microscopy.Results There were no differences between PCOS and control groups with respect to IVM rates (50.0% and 42.9%, respectively) nor the percentage of meiotic abnormalities in metaphase II oocytes (35.3% and 25%, respectively). Conclusions In vitro-matured oocytes obtained from stimulated cycles of nonobese PCOS did not have an increased ratio of meiotic abnormalities.

In Vitro Maturation in Women with vs. without Polycystic Ovarian Syndrome: A Systematic Review and Meta-Analysis

“…The full text of these studies was examined thoroughly, resulting in the exclusion of 91 (Table A in S2 File ). Eventually, 11 studies were considered eligible for inclusion in the meta-analysis [ 11 – 13 , 18 , 23 , 34 – 39 ]. Characteristics of the studies included are presented in Table 1 .…”

“…The two largest studies published so far investigating the research question were by Child et al and Junk et al conducted in 2001 and 2012, respectively [ 12 , 36 ], involving 99 PCOS patients and 108 controls in total. The comparison group consisted of PCO women in five studies [ 11 – 13 , 34 , 36 ] and in eight of women/couples suffering from other causes of sub-fertility [ 12 , 13 , 18 , 23 , 34 , 37 – 39 ].…”

“…HCG was used to trigger final oocyte maturation in five studies [ 12 , 13 , 23 , 37 , 39 ], mild stimulation with FSH was used in five studies [ 35 – 39 ], while in three no hormonal priming was used [ 11 , 18 , 34 ] ( Table 2 ). No studies reported on ovarian hyperstimulation syndrome and preterm birth, whereas one study reported on congenital abnormalities [ 23 ].…”

In vitro maturation of cumulus–partially enclosed immature human oocytes by priming with gonadotropin

“…1 B1-B3). Meiotic aberrations consisted of abnormal spindle configuration and/or abnormal chromosomal organizations (13).…”

Confocal Microscopic Analysis of the Spindle and Chromosome Configurations of in vitro-Matured Oocytes from Different Types of Polycystic Ovary Syndrome Patients

“…However, all oocytes of the cited study were obtained from PCOS women, and the in vitro-matured oocytes analyzed in the study were obtained from unstimulated cycles. Another published report evaluated the meiotic spindle and chromosome distribution of in vitro-matured oocytes from infertile nonobese women with PCOS and subjects with male or tubal causes of infertility (controls) and found that in vitro-matured oocytes obtained from stimulated cycles of nonobese PCOS patients did not have an increased ratio of meiotic abnormalities [38]. The proportion of meiotic anomalies observed (35.3%) was similar to that found in the present study (42.9% spindle anomalies in the nonobese group).…”