Comparative analysis of the Metarhizium anisopliae secretome in response to exposure to the greyback cane grub and grub cuticles

Manalil, Nirupama Shoby; Te’o, Junior; Braithwaite, Kathy; Brumbley, Stevens M.; Samson, Peter R.; Nevalainen, Helena

doi:10.1016/j.funbio.2010.05.005

Cited by 10 publications

(12 citation statements)

References 39 publications

(40 reference statements)

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“…The strategy of applying synthetic media to induce the activation of the infection systems has been applied with success, revealing genes and proteins involved in different stages of Metarhizium anisopliae ’s infection process. 7,12,43,44 Several articles have been reporting poor correlation between mRNA and protein levels. 45−47 Most of the work in M. anisopliae has analyzed gene expression, which makes the identification of secreted proteins difficult.…”

Section: Discussionmentioning

confidence: 99%

“…49 Only a few proteomic studies have been published on M. anisopliae and all applied low-throughput techniques. 12,43,50−54 By applying shotgun proteomics, 21 we were able to identify 71 proteins differentially expressed under infection conditions. Most of these proteins were not detected in other proteomic experiments about insect infection.…”

Section: Discussionmentioning

confidence: 99%

“…Most of these proteins were not detected in other proteomic experiments about insect infection. 12,43,52,53 Multiple molecular functions were assigned to these 71 proteins by GO annotation, including several known to be involved in M. anisopliae infection Table 4 (hydrolase activity, enzyme inhibitor activity, oxidoreductase activity, superoxide dismutase activity, and protein and carbohydrate binding). 6−9 The infection process occurs in three stages: (1) adhesion to the cuticle, (2) penetration of the cuticle, followed by (3) host colonization of the internal tissues.…”

Section: ■ Discussionmentioning

confidence: 99%

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Secretome of the Biocontrol AgentMetarhizium anisopliaeInduced by the Cuticle of the Cotton PestDysdercus peruvianusReveals New Insights into Infection

Beys‐da‐Silva¹,

Santi²,

Berger

et al. 2014

J. Proteome Res.

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Metarhizium anisopliae is an entomopathogenic fungus that has evolved specialized strategies to infect insect hosts. Here we analyzed secreted proteins related to Dysdercus peruvianus infection. Using shotgun proteomics, abundance changes in 71 proteins were identified after exposure to host cuticle. Among these proteins were classical fungal effectors secreted by pathogens to degrade physical barriers and alter host physiology. These include lipolytic enzymes, Pr1A, B, C, I, and J proteases, ROS-related proteins, oxidorreductases, and signaling proteins. Protein interaction networks were generated postulating interesting candidates for further studies, including Pr1C, based on possible functional interactions. On the basis of these results, we propose that M. anisopliae is degrading host components and actively secreting proteins to manage the physiology of the host. Interestingly, the secretion of these factors occurs in the absence of a host response. The findings presented here are an important step in understanding the host–pathogen interaction and developing more efficient biocontrol of D. peruvianus by M. anisopliae.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Secretome of the Biocontrol AgentMetarhizium anisopliaeInduced by the Cuticle of the Cotton PestDysdercus peruvianusReveals New Insights into Infection

Beys‐da‐Silva¹,

Santi²,

Berger

et al. 2014

J. Proteome Res.

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“…The cuticles were introduced to the fungal hyphae as a powder as described earlier (e.g., Fang et al, 2009; Manalil et al, 2010) to gain enough fungus material even though it may cause biases. However, using an in vivo system (fungus developing on whole ticks) might result in inadequate fungus material but also in masking fungus sequences due to higher abundance of host sequences.…”

Section: Methodsmentioning

confidence: 99%

Comparative response of Metarhizium brunneum to the cuticles of susceptible and resistant hosts

Ment

Gindin

Samish

et al. 2020

Arch Insect Biochem Physiol

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Earlier studies demonstrated that Metarhizium brunneum, usually a broad-host pathogen of arthropods, is unable to complete its pathogenic life cycle when inoculated on the fungus-resistant tick, Hyalomma excavatum engorged females. While the fungus penetrates the cuticle of fungussusceptible tick, Rhipicephalus annulatus females, it is unable to penetrate the cuticle of fungus-resistant tick, and even perishes on its surface. This is probably due to high concentration of antifungal fatty acids and probably also due to a hypersensitive-like response of the tick. To understand the metabolic pathways occurring in the fungal hyphae upon encountering the cuticles, we compared the response of the fungus to cuticle from susceptible and resistant tick cuticles by 2D-gels. The intracellular proteomes of M. brunneum Mb7 exposed to cuticle of the fungus-susceptible tick, R. annulatus, and to the fungusresistant tick, H. excavatum engorged females were compared after exposure to either cuticles. By means of liquid chromatography-mass spectrometry/mass spectrometry we identified in both proteomes common proteins involved in biological processes as well as unique proteins identified only in the proteome of fungus exposed to fungus-resistant tick cuticle. These proteins were identified in high probability as heat shock proteins, four key enzymes of the glyoxylate cycle, and proteins associated with hypoxia, and exposure to antifungal drugs. These findings are discussed

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“…Cleared supernatants were then aliquoted in 1.5 ml Eppendorf tubes and stored at -80°C. Fungal protease inhibitor cocktail (0.05% v/v, Sigma-Aldrich, Australia) was added in the culture supernatant samples used for proteomic analysis [36]. …”

Section: Methodsmentioning

confidence: 99%

Secretion of Proteases by an Opportunistic Fungal Pathogen Scedosporium aurantiacum

2017

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Scedosporium aurantiacum is an opportunistic filamentous fungus increasingly isolated from the sputum of cystic fibrosis patients, and is especially prevalent in Australia. At the moment, very little is known about the infection mechanism of this fungus. Secreted proteases have been shown to contribute to fungal virulence in several studies with other fungi. Here we have compared the profiles of proteases secreted by a clinical isolate Scedosporium aurantiacum (WM 06.482) and an environmental strain (WM 10.136) grown on a synthetic cystic fibrosis sputum medium supplemented with casein or mucin. Protease activity was assessed using class-specific substrates and inhibitors. Subtilisin-like and trypsin-like serine protease activity was detected in all cultures. The greatest difference in the secretion of proteases between the two strains occurred in mucin-supplemented medium, where the activities of the elastase-like, trypsin-like and aspartic proteases were, overall, 2.5–75 fold higher in the clinical strain compared to the environmental strain. Proteases secreted by the two strains in the mucin-supplemented medium were further analyzed by mass spectrometry. Six homologs of fungal proteases were identified from the clinical strain and five from the environmental strain. Of these, three were common for both strains including a subtilisin peptidase, a putative leucine aminopeptidase and a PA-SaNapH-like protease. Trypsin-like protease was identified by mass spectrometry only in the clinical isolate even though trypsin-like activity was present in all cultures. In contrast, high elastase-like activity was measured in the culture supernatant of the clinical strain but could not be identified by mass spectrometry searching against other fungi in the NCBI database. Future availability of an annotated genome will help finalise identification of the S. aurantiacum proteases.

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Comparative analysis of the Metarhizium anisopliae secretome in response to exposure to the greyback cane grub and grub cuticles

Cited by 10 publications

References 39 publications

Secretome of the Biocontrol AgentMetarhizium anisopliaeInduced by the Cuticle of the Cotton PestDysdercus peruvianusReveals New Insights into Infection

Secretome of the Biocontrol AgentMetarhizium anisopliaeInduced by the Cuticle of the Cotton PestDysdercus peruvianusReveals New Insights into Infection

Comparative response of Metarhizium brunneum to the cuticles of susceptible and resistant hosts

Secretion of Proteases by an Opportunistic Fungal Pathogen Scedosporium aurantiacum

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