Comparative analysis of the gut microbiota of Apis cerana in Yunnan using high-throughput sequencing

Luo, Zhengxiu; Dong, Zhi-Xiang; Chen, Yifei; Li, Huan-Yuan; Tang, Qihe; Li, Jilian; Guo, Jun

doi:10.1007/s00203-020-01974-0

Cited by 10 publications

(7 citation statements)

References 49 publications

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“…Our results demonstrated that the gut bacterial communities of A. cerana adult workers comprise four major phyla, Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria. This result is consistent with Luo et al’s (2020) reports. At the genus level, previous studies showed that Lactobacillus , Snodgrassella , and Gilliamella were the major genera ( Guo et al, 2015 ; Huang et al, 2018 ; Luo et al, 2020 ).…”

Section: Discussionsupporting

confidence: 94%

“…This result is consistent with Luo et al’s (2020) reports. At the genus level, previous studies showed that Lactobacillus , Snodgrassella , and Gilliamella were the major genera ( Guo et al, 2015 ; Huang et al, 2018 ; Luo et al, 2020 ). In addition to this, we found Bombella , Pectinatus, and Apibacter also have very high abundance in our samples.…”

Section: Discussionsupporting

confidence: 94%

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Gut microbiota composition and gene expression changes induced in the Apis cerana exposed to acetamiprid and difenoconazole at environmentally realistic concentrations alone or combined

Wang

et al. 2023

Front. Physiol.

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Apis cerana is an important pollinator of agricultural crops in China. In the agricultural environment, A. cerana may be exposed to acetamiprid (neonicotinoid insecticide) and difenoconazole (triazole fungicide), alone or in combination because they are commonly applied to various crops. At present, our understanding of the toxicological effects of acetamiprid and difenoconazole on honey bee gut microbiomes is limited. The primary objective of this study was to explore whether these two pesticides affect honey bees’ gut microbiota and to analyze the transcriptional effects of these two pesticides on honey bees’ head and gut. In this study, adults of A. cerana were exposed to acetamiprid and/or difenoconazole by contaminated syrup at field-realistic concentrations for 10 days. Results indicated that acetamiprid and/or difenoconazole chronic exposure did not affect honey bees’ survival and food consumption, whereas difenoconazole decreased the weight of honey bees. 16S rRNA sequencing suggested that difenoconazole and the mixture of difenoconazole and acetamiprid decreased the diversity index and shaped the composition of gut bacteria microbiota, whereas acetamiprid did not impact the gut bacterial community. The ITS sequence data showed that neither of the two pesticides affected the fungal community structure. Meanwhile, we also observed that acetamiprid or difenoconazole significantly altered the expression of genes related to detoxification and immunity in honey bees’ tissues. Furthermore, we observed that the adverse effect of the acetamiprid and difenoconazole mixture on honey bees’ health was greater than that of a single mixture. Taken together, our study demonstrates that acetamiprid and/or difenoconazole exposure at field-realistic concentrations induced changes to the honey bee gut microbiome and gene expression.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 94%

Gut microbiota composition and gene expression changes induced in the Apis cerana exposed to acetamiprid and difenoconazole at environmentally realistic concentrations alone or combined

Wang

et al. 2023

Front. Physiol.

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“…Also, Iorizzo et al (2020) reported the predominance of Lactiplantibacillus plantarum in the gut of Apis mellifera in Italy. According to a chineese study (Luo et al 2020) the abundance of lactobacilli and other species was high in Apis cerana from different areas. Almost half of the Apilactobacillus kunkeei isolates identi ed in our study were detected in BGIT.…”

Section: Discussionmentioning

confidence: 99%

Effect of honey bee forage plants in Tunisia on diversity and antibacterial potential of lactic acid bacteria and bifidobacteria from Apis mellifera intermissa and its products

Ben-Miled

Semmar

Castellanos

et al. 2023

Preprint

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Lactic acid bacteria and bifidobacteria (LAB & Bifido), isolated from the gastrointestinal tract of Apis mellifera intermissa (BGIT), honey (H), propolis (P) and bee bread (BB) of hives set in different vegetations (wildflowers, caraway, orange blossom, Marrubium vulgare, Eucalyptus and Erica cinerea), were subjected to analysis of their antibacterial potential. Isolates able to inhibit Staphylococcus aureus were selected and identified with MALDI-TOF MS leading to 154 strains representing 12 LAB & Bifido species. Lactiplantibacillus plantarum, Pediococcus pentosaceus and Enterococcus faecalis were predominantly found in all matrices. BGIT showed the highest LAB & Bifido diversity with exclusive occurrences of five species (including Bifidobacterium asteroides and Limosilactobacillus fermentum). Honey was the second origin harboring an important variety of LAB species of which Apilactobacillus kunkeei and Enterococcus mundtii were characteristic of both H and BGIT. Principal components analysis revealed associations between antibacterial activities of LAB & Bifido, matrices and honey bee forage plants. Inhibition trends of S. aureus and Citrobacter freundii were highlighted with: Lactiplantibacillus plantarum from BGIT, P, H of bees feeding on Erica cinerea; Pediococcus pentosaceus from BGIT, P, BB associated with Erica cinerea; and Bifidobacterium asteroidesfrom BGIT/orange blossom system. However, Enterococcus faeciumassociated with BGIT/Eucalyptus system antagonized Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii. Our findings highlighted noteworthy effects of bee forage plants on the antibacterial activity of LAB & Bifido. Our approach could be useful to identify multiple conditions promoting antibacterial potency of LAB & Bifido under the combined effects of feeding plants and living matrices.

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“…Its relative abundance revealed a key role for Gilliamella as a core genus in workers [ 30 ]. They are endosymbionts and are the most critical types of fermentation bacteria in the honeybee intestine, protecting honeybees from pathogens and improving dietary tolerances [ 28 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…Lactobacillus is the most critical probiotic genus in the digestive tract of honeybees, with essential roles in carbohydrate metabolism [ 40 ] and protecting hosts through producing antimicrobial metabolites (such as organic acids, diacetyl, benzoate, and bacteriocins) and induction of immune responses [ 2 , 6 , 30 , 39 ]. Recent studies showed that L. Firm -4 and L. Firm -5 hosted numerous phosphotransferase systems that participated in the digestion of sugars, particularly in L. Firm-5.…”

Section: Discussionmentioning

confidence: 99%

The Gut Microbiota at Different Developmental Stages of Apis cerana Reveals Potential Probiotic Bacteria for Improving Honeybee Health

et al. 2022

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Honeybees play a vital role in the ecological environment and agricultural economy. Increasing evidence shows that the gut microbiome greatly influences the host’s health. Therefore, a thorough understanding of gut bacteria composition can lead to the development of probiotics specific for each development stage of honeybees. In this study, the gut microbiota at different developmental stages (larvae, pupae, and adults) of the honeybees Apis cerana in Hanoi, Vietnam, was assessed by sequencing the V3–V4 region in the 16S rRNA gene using the Illumina Miseq platform. The results indicated that the richness and diversity of the gut microbiota varied over the investigated stages of A. cenara. All three bee groups showed relative abundance at both phylum and family levels. In larvae, Firmicutes were the most predominant (81.55%); however, they decreased significantly along with the bee development (33.7% in pupae and 10.3% in adults) in favor of Proteobacteria. In the gut of adult bees, four of five core bacteria were found, including Gilliamella apicola group (34.01%) Bifidobacterium asteroides group (10.3%), Lactobacillus Firm-4 (2%), and Lactobacillus Firm-5 (1%). In contrast, pupae and larvae lacked almost all core bacteria except G. apicola (4.13%) in pupae and Lactobacillus Firm-5 (4.04%) in larvae. This is the first report on the gut microbiota community at different developmental stages of A. cerana in Vietnam and provides potential probiotic species for beekeeping.

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Comparative analysis of the gut microbiota of Apis cerana in Yunnan using high-throughput sequencing

Cited by 10 publications

References 49 publications

Gut microbiota composition and gene expression changes induced in the Apis cerana exposed to acetamiprid and difenoconazole at environmentally realistic concentrations alone or combined

Gut microbiota composition and gene expression changes induced in the Apis cerana exposed to acetamiprid and difenoconazole at environmentally realistic concentrations alone or combined

Effect of honey bee forage plants in Tunisia on diversity and antibacterial potential of lactic acid bacteria and bifidobacteria from Apis mellifera intermissa and its products

The Gut Microbiota at Different Developmental Stages of Apis cerana Reveals Potential Probiotic Bacteria for Improving Honeybee Health

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