Comparative analysis of the effects of Copaifera multijuga oil-resin and nitrofurazona in the cutaneous wound healing process

Martini, Carlos Augusto Nunes; Scapini, João Guilherme Seifert; Collaço, Luiz Martins; Matsubara, Anderson; Júnior, Valdir Florêncio da Veiga

doi:10.1590/0100-69912016006006

Cited by 13 publications

(6 citation statements)

References 16 publications

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“…The positive regulatory effects of copaiba essential oil peaked at 30 min with an EC 50 of approximately 80 ng/mL and were mediated in part by CB2. The positive effects of copaiba essential oil on neuronal signaling pathways were consistent with its reported functions in metabolism [32], wound healing [33][34][35][36], and anti-inflammation [37][38][39][40][41]. Interestingly, copaiba essential oil also activated the apoptosis signaling pathway in a time-dependent manner and reduced the viability of neuronal cells with an EC 50 of approximately 400 ng/mL.…”

Section: Discussionsupporting

confidence: 78%

Fast-Acting and Receptor-Mediated Regulation of Neuronal Signaling Pathways by Copaiba Essential Oil

Urasaki

Beaumont²,

Workman³

et al. 2020

IJMS

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This study examined the biological activities of copaiba essential oil via measurement of its effects on signaling pathways in the SH-SY5Y neuronal cell line. Nanofluidic proteomic technologies were deployed to measure the phosphorylation of biomarker proteins within the signaling cascades. Interestingly, copaiba essential oil upregulated the pI3K/Akt/mTOR, MAPK, and JAK/STAT signaling pathways in neuronal cells. The effects of copaiba essential oil peaked at 30 min post-treatment, with a half-maximal effective concentration (EC50) of approximately 80 ng/mL. Treatment with cannabinoid receptor 2 (CB2) agonist AM1241 or the inverse agonist BML190 abrogated the regulatory effects of copaiba essential oil on the pI3K/Akt/mTOR signaling pathway. Surprisingly, copaiba essential oil also activated the apoptosis signaling pathway and reduced the viability of SH-SY5Y cells with an EC50 of approximately 400 ng/mL. Furthermore, β-caryophyllene, a principal constituent of copaiba essential oil, downregulated the pI3K/Akt/mTOR signaling pathway. Taken together, the findings indicated that copaiba essential oil upregulated signaling pathways associated with cell metabolism, growth, immunity, and apoptosis. The biological activities of copaiba essential oil were determined to be fast acting, CB2 mediated, and dependent on multiple chemical constituents of the oil. Nanofluidic proteomics provided a powerful means to assess the biological activities of copaiba essential oil.

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Section: Discussionsupporting

confidence: 78%

Fast-Acting and Receptor-Mediated Regulation of Neuronal Signaling Pathways by Copaiba Essential Oil

Urasaki

Beaumont²,

Workman³

et al. 2020

IJMS

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“…Copaiba oil also showed positive effects on bladder healing, 11 and skin healing processes. 27 In ►Table 5, we see a predominance of in vitro research, with 50%, followed closely by the publications of clinical studies in animals, with 40.4%. In the papers evaluated in the present meta-analysis, we could see that copaiba oil is promising in the medical field as a very great potential alternative therapy as an antimicrobial, 19 anti-inflammatory, 1 as well as an antitumor agent.…”

Section: Resultsmentioning

confidence: 93%

Meta-analysis on Copaiba Oil: Its Functions in Metabolism and Its Properties as an Anti-inflammatory Agent

et al. 2018

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Recent studies demonstrated that one of the major components of the copaiba oil, β-caryophyllene, has an anti-inflammatory effect. 4 Copaiba oil is extracted from the copaifera, a Abstract Many medicinal plants are sought as alternative therapies for various conditions, as opposed to synthetic alternatives. The Brazilian Amazon provides a plethora of plants with various therapeutic and medicinal properties. One of them is copaiba oil, a natural product of the biodiversity of the Amazon. The anti-inflammatory and antimicrobial properties of copaiba oil have been drawing the attention of the scientific community as a great alternative in the treatment of illnesses associated with inflammatory and infectious processes. The purpose of the present study is to evaluate and analyze the latest publications on the use of copaiba oil in the PubMed/Medline database from 2014 to 2017. Forty-two articles were collected, verifying anti-inflammatory activity, antimicrobial activity, healing, and antitumor activity. The results found on researches with copaiba oil according to publication dates were 11.9% in 2014, 38.1% in 2015, 16.7% in 2016, and 33.3% in 2017. In the analyzed studies, the proposed administration of copaiba oil was 50% topical, 28.6% oral, 9.5% enteral, 4.8% subcutaneous, and 7.1% others. In the objectives of the presente study in relation to the administration of copaiba oil, the result was 31% antimicrobial, 28.6% anti-inflammatory, 9.5% antitumor, 9.5% healing, and 21% others. Regarding the types of research, we have found 50% in vitro, 40.4% in vivo animals, 2.4% in vivo human, 2.4% review and 4.8% others. Regarding the significance factor evaluation, the result was 100% positive in antitumor activity, 84% positive in infection and inflammation pathology, 62% positive in others, like dental processes, food technology application support and as a drug absorption vehicle, and 50% in healing. The results presented in the current meta-analysis show that copaiba oil is an effective alternative to treatments of illnesses such as chronic inflammation, infectious diseases, various types of cancer, autoimmune diseases, and as a vehicle for the absorption of other drugs. Thus, more studies regarding the benefits of copaiba oil could provide a great contribution to the medical community in the treatment of the aforementioned illnesses.

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“…Subsequently, type III collagen is progressively replaced by thicker, more resistant and densely organized Type I collagen fibers (22). Thus, increasing the type I/III collagen relation over the time-course of wound healing is required to provide tensile strength and mechanical stability to the fibrous scar (23).…”

Section: Discussionmentioning

confidence: 99%

Effects of Different Protocols of Low-Level Laser Therapy on Collagen Deposition in Wound Healing

et al. 2019

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The low-level laser has proven successful in stimulating the production of collagen in wound healing assays. However, diversity has been observed in the protocols used. This work has evaluated the effects of three protocols of low-level laser therapy (LLLT) in the healing of open wounds in rats. Standard-sized wounds of 1 cm2 were performed with a scalpel in the middorsal region of 60 male Wistar rats weighing 225±25 g, and they were assigned into four groups (n=15): CTR (non-irradiated animals), LT1 (20 J/cm2 daily), LT2 (16 J/cm2 daily) and LT3 (20 J/cm2 daily). After 7, 14 and 21 days, five animals/day were euthanized and the wounds analyzed histologically. Data were subjected to normality analysis of distribution using Shapiro-Wilk test. Gaussian data were analyzed using ANOVA and Bonferroni tests whereas non-Gaussian data were analyzed using Kruskal-Wallis and Dunn tests, considering significant p values less than 0.05. The LLLT in all protocols reduced the inflammation and collagen deposition increased significantly (p<0.05). However, LT2 showed the highest levels of collagen in all phases of the study (p<0.05) induced faster replacement of immature collagen III by mature collagen I in the early stages of repair and early collagen remodeling promoted by providing better organization architectural beams deposited. It was concluded that all protocols induced an increase in collagen scar. However, the protocol 2 (16 J /cm2, daily application) promoted the most significant increases in collagen deposition, accelerated maturation of collagen and showed the best architecture of the final fibrous scarring.

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Comparative analysis of the effects of Copaifera multijuga oil-resin and nitrofurazona in the cutaneous wound healing process

Cited by 13 publications

References 16 publications

Fast-Acting and Receptor-Mediated Regulation of Neuronal Signaling Pathways by Copaiba Essential Oil

Fast-Acting and Receptor-Mediated Regulation of Neuronal Signaling Pathways by Copaiba Essential Oil

Meta-analysis on Copaiba Oil: Its Functions in Metabolism and Its Properties as an Anti-inflammatory Agent

Effects of Different Protocols of Low-Level Laser Therapy on Collagen Deposition in Wound Healing

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