Comparative Analysis of Rapid Dengue Testing and ELISA for NS1 Antigen and IgM in Acute Dengue Infection

Reddy, R.; Sahai, Kavita; Malik, Ajay; Shoba, S.; Khera, Anurag

doi:10.20546/ijcmas.2016.510.100

Cited by 5 publications

(5 citation statements)

References 10 publications

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“…The sensitivity of Rapid ICT when compared to ELISA was found to be 93.6% and specificity of 100% which corroborates with the results of Mahesh Reddy et al 19 in 2016 (90.1% sensitivity and 98.45% specificity). Similar results were also observed by Groen et al 20 ., (2000) and Shih et al 21 (2016).…”

Section: Resultssupporting

confidence: 87%

Comparison of the Diagnostic Efficacy of Rapid Immunochromatographic Test with Microwell ELISA in the detection of Dengue NS1 Antigen - A Retrospective Study

Krithikaa¹,

Raj²,

arasi³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Section: Resultssupporting

confidence: 87%

Comparison of the Diagnostic Efficacy of Rapid Immunochromatographic Test with Microwell ELISA in the detection of Dengue NS1 Antigen - A Retrospective Study

Krithikaa¹,

Raj²,

arasi³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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“…In our study, rapid ICT assay for NS1 Ag and IgM shows sensitivity more than 80% and specificity 100% compared to ELISA. Another study by Reddy RM showed over 90% sensitivity and over 98% specificity 12 While studies conducted in various hospitals recorded a good range of sensitivity of rapid ICT for NS1 Ag 49.4%-98.9% and specificity starting from 91%-100%. 13 Sensitivity of 82.14% and specificity of 100% were observed in our study for IgM antibody detection by rapid ICT in comparison to ELISA during this study.…”

Section: Discussionmentioning

confidence: 93%

Diagnostic Efficacy of Rapid Immunochromatographic Test in Diagnosis of Dengue Infection

Kaur¹,

Sharma²

2022

J. Pure Appl. Microbiol.

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Dengue is a mosquito-borne arboviral disease of grave public health concern worldwide. Early diagnosis and treatment is required to reduce morbidity & mortality from complications caused by secondary dengue infection. According to WHO, the three main diagnostic modalities for the diagnosis of dengue infection are cultivation and identification of viruses, molecular methods, and serology. Whereas virus cultivation is labour intensive and available only in reference laboratories, molecular methods require expensive infrastructure & expertise. Serology on the other hand not only less tedious but is also able to differentiate between primary and secondary dengue. This study was undertaken to evaluate the diagnostic efficacy of rapid immunochromatographic assay in the diagnosis of dengue infection as compared to ELISA. The study was conducted in the serology section of the Microbiology laboratory, Sri Guru Ram Das Institute of Medical Sciences, Amritsar. Blood samples from 429 patients with clinical suspicion of dengue virus infection were received in the lab from August 2020 to December 2020. All samples were subjected to rapid ICT and ELISA to detect NS1 Ag and IgM antibodies. The majority number of cases were observed in the age group of 31 to 40 years while the gender-wise ratio was 1.43:1 showing male preponderance. Out of 429 samples tested, 156 were reactive for either NS1 antigen or IgM antibodies by the ELISA method. Results of rapid ICT for NS1Ag and results of NS1Ag by ELISA were analyzed and compared. A sensitivity of 81.25% was noted and specificity of 100%. IgM detection by rapid ICT in comparison to IgM ELISA shows a sensitivity of 82.14% and specificity of 100%. Rapid ICT kits performed at par with the ELISA. Rapid immunochromatographic assays are important diagnostic tools in the identification of dengue and early treatment of dengue patients is possible, reducing mortality significantly.

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“…A study done by Mahesh Reddy R. 11 showed that out of 740 sera samples 354 were NS1 Ag ELISA positive and out of 609 sera samples, 269 were IgM antibody positive. Most of the cases who were Dengue positive either by NS1Antigen or IgM antibody were between the ages of 10-50 years of age.…”

Section: Resultsmentioning

confidence: 99%

Comparative evaluation of dengue NS1 antigen rapid test and IgM ELISA in a tertiary care hospital in VIMS, Ballari

Kalpana

Varma

2021

IJMMTD

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Dengue virus infection is becoming a major public health problem in recent decades because of the mortality and morbidity associated with it. Dengue is endemic in many parts of India and epidemics are frequently reported from various parts of India and abroad. The mortality rate in patients with dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) is as high as 44%. Hence early and rapid laboratory diagnosis of dengue is crucial. Appropriate clinical management can save the lives of DHF and DSS patients and mortality can be reduced to less than 1%. It is also worthwhile for planning appropriate control strategies. The present communication reports the seroprevalence of dengue infections occurred in Central India.To detect Dengue infection by NS1 Antigen Rapid detection and by IgM ELISA and compare Rapid NS1 Antigen detection test and IgM ELISA test.Blood samples from 5273 suspected cases were collected from January 2016 to December 2016. Sera were separated and subjected for NS1 antigen detection testing by the solid phase immunochromatographic assay, a commercial dengue virus rapid test kit. and antidengue IgM antibody ELISA in the Department of Microbiology, VIMS, Ballari. All samples were put for ELISA.3036 samples were tested for NS1 Antigen detection rapid method.2272 samples were tested for both rapid test and ELISA. Out of 5273 cases, 446 (8.46%) were found to be positive by IgM ELISA. 3036 samples WHICH were tested for NS1 Antigen detection by rapid method 208 (6.85%)samples were positive. The most affected age group was 5-10yrs with 284 cases, out of which 29 were positive, followed by the age group 0f 15yrs and above with 8.74%. Males were affected more than females with a percentage of 9.2 and 7.4 respectively. The highest number of suspected dengue patients admitted was in the month of November, i.e., 459 with 68 positive (14.81%) followed by August (12.16%) and September (11.03%), i.e., 28 with 7 (25%) positive. For the 2272 samples for which both tests were done, 153 cases were positive for rapid test and 137 cases were positive by ELISA. Dengue cases were more during September to November in the post monsoon season which is useful to plan special preventive strategies. The study draws attention toward the male, young and adult age group. To conclude, in countries lacking infrastructure for the diagnostic labs especially in the rural and remote areas, the rapid dengue ICT tests can play a major role in diagnosis and in patient management of acute dengue infection. The rapid ICTs are very simple, easy to perform, and can be used as point of care tests. We suggest that the rapid ICT for NS1 Ag detection may be used in patients presenting with fever less than 5 days

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Comparative Analysis of Rapid Dengue Testing and ELISA for NS1 Antigen and IgM in Acute Dengue Infection

Cited by 5 publications

References 10 publications

Comparison of the Diagnostic Efficacy of Rapid Immunochromatographic Test with Microwell ELISA in the detection of Dengue NS1 Antigen - A Retrospective Study

Comparison of the Diagnostic Efficacy of Rapid Immunochromatographic Test with Microwell ELISA in the detection of Dengue NS1 Antigen - A Retrospective Study

Diagnostic Efficacy of Rapid Immunochromatographic Test in Diagnosis of Dengue Infection

Comparative evaluation of dengue NS1 antigen rapid test and IgM ELISA in a tertiary care hospital in VIMS, Ballari

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