Comparative analysis of radical scavenging and antioxidant activity of phenolic compounds present in everyday use spice plants by means of spectrophotometric and chromatographic methods

Abstract

Comparative analysis of radical scavenging and antioxidant activities of phenolic compounds present in everyday use spice plants was carried out by means of spectrophotometric and chromatographic methods. Six spice plant samples, namely onion (Allium cepa), parsley (Petroselinum crispum) roots and leaves, celery (Apium graveolens) roots and leaves and leaves of dill (Anethum graveolens) were analyzed. Total amount of phenolic compounds and radical scavenging activity (RSA) was the highest in celery leaves and dill extracts and was the lowest in celery roots. Comparing commonly used spectrophotometric analysis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) RSA of extracts with the results obtained using reversed-phase chromatographic separation with on-line post-column radical scavenging reaction detection, good correlation was obtained (R(2)=0.848). Studies using HPLC system with electrochemical detector showed that bioactive phytochemicals can be separated and antioxidant activities of individual compounds evaluated without the need of a complex HPLC system with reaction detector. The results obtained using electrochemical detection correlate with the RSA assayed using spectrophotometric method (R(2)=0.893).

Variation of total amount of phenolic compounds, radical scavenging activity and volatile com pounds of Liriodendron tulipifera L. and Ginkgo biloba L. leaves extracts during different vegetation periods

“…The method reported by Stankevičius et al [26] was slightly modified and used to determine the total amount of phenolic compounds (TAP) in the extracts. The plant extracts were diluted 10 times with 75% (vol.)…”

“…The method was described earlier in [26]. For analysis, 20 µl of sample was injected by means of model 542 auto sampler.…”

Volatile compounds composition and antioxidant activity of bee pollen collected in Lithuania

“…It should also be of particular interest for the analysis of the primary antioxidants, which are all electroactive (Pyo et al, 2004). HPLC-ECD has been used for the analysis of many food products, such as beer, wine, tea, yacon (Jandera et al, 2005), spices (Stankevičius et. al., 2011), vegetables and fruits (Sochor et al, 2013); however, this is the first study about the use of HPLC-ECD for the screening of bee pollen antioxidant characteristics.…”

“…The on-line HPLC-DPPH method allows to determine compounds responsible for the DPPH radical scavenging activity in the sample (Damašius et al, 2014), while HPLC-ECD is suitable for specific determination of electrochemically active compounds (Stankevičius et al, 2011). HPLC coupled with electrochemical detection has become a widely accepted and valuable technique because of its high sensitivity as well as superior selectivity to UV absorption for electrochemically oxidizable analytes (Vanbeneden et al, 2006).…”

Determination of pesticide residues in complex matrices using multi‐walled carbon nanotubes as reversed‐dispersive solid‐phase extraction sorbent

“…They all contain a large amount of sulfur-containing compounds that may bring serious matrix effects and significant interferences in MS. To deal with the complex matrices, there are a number of extraction or cleanup techniques for pesticide, pharmaceutical, warfare agent analysis: microwave pretreatment [1,2], supercritical fluid extraction [3], gel-permeation chromatography [4], solid-phase microextraction [5] and matrix solidphase dispersion [6]. Some other methods were also studied such as solid-phase extraction [3,7], pressurized liquid extraction [8], reversed-phase chromatographic separation [9], high-performance thin-layer chromatography [10], microwave hydrodiffusion and gravity [11], solid-phase microextraction [12], etc. Both advantages and disadvantages could be found in those methods.…”