Comparative analysis of polygalacturonases in isolates of seven species of Fusarium from Pinus pinea

A single amino acid substitution in highly similar endo-PGs from Fusarium verticillioides and related Fusarium species affects PGIP inhibition

“…F. moniliforme Sheldon) is the most intensively studied CWDE thus far (Caprari et al, 1993;Posada et al, 2000Posada et al, , 2001Daroda et al, 2001). PG degrades the a-1,4 galacturonosyl bonds of the homogalacturonan regions of pectins within the middle lamella and primary cell wall, and may facilitate the growth of hyphae within the plant tissue.…”

Production of Cell Wall Degrading Enzymes by Isolates of Bipolaris maydis, the Causative Agent of Maydis Leaf Blight of Maize

“…Verlant et al, (2004) reported that the pectin methyl esterase is a pectin degrading enzymes that partially demethoxylated chains of pectin and methanol. Posada et al, (2001) investigated that polygalacturonases enzyme produced by phytopathogenic fungi helps in the process of infection and colonization in the infected host tissue. In esterase activity, the isolate E15 showed the highest activity index (H/C=1.40) and least activity index was observed in E6 and E19 (H/C=1.12).…”

Induction of Extracellular Lytic Enzymes by <i>Fusarium solani</i>

“…It has been reported that F. oxisporum produces a variety of lytic enzymes, which depolymerize all components of plant cell walls, such as cellulose, xylan, pectin, polygalacturonic acids and proteins (extensins); and they have been purified and biochemically characterized as several enzymes, one endopolygalacturonase majority (PG1), two exopolygalacturonase (PG2 and PG3), one endoxylanase (XYL1), one endopectate lyase (PL1) [6,7], seven polygalacturonases of Fusarium species of Pinus pinea [8], and the isolation of differentially expressed genes during interactions between tomato cells and a strain of F. oxysporum [9]. With respect to F. solani, studies have reported the isolation of a novel pectate lyase gene from the phytopathogenic fungus F. solani [10], a F. solani mutant recurring in cutinase activity and virulence [11], extracellular lipase by the phytopathogenic fungus F. solani FS1 [12], and a cellulose of F. solani [13].…”