Comparative Analysis of Pdf-Mediated Circadian Behaviors Between<i>Drosophila melanogaster</i>and<i>D. virilis</i>

Bahn, Jae Hoon; Lee, Gyunghee; Park, Jae H.

doi:10.1534/genetics.108.099069

Cited by 72 publications

(80 citation statements)

References 63 publications

(77 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This construct was expressed in a somewhat broader set of circadian neurons with the dvpdfgal4 driver; i.e., all PDF cells, LNds, and the PDF-negative fifth s-LNv (Bahn et al 2009). These flies also exhibit long periods, 1.3-h longer than control flies (Supplemental Fig.…”

Section: Usp8 Is Important For Locomotor Activity Rhythms In Vivomentioning

confidence: 99%

“…All gal4 and gal80ts drivers used in this study have been described previously: timgal4 , pdfgal4 (Stoleru et al 2004), dvpdfgal4 (Bahn et al 2009), tubgal80ts (McGuire et al 2003, usp8-RNAi R2 (Mukai et al 2010), and usp8-RNAi (HMS01889, Bloomington). uas-usp8-DN transgenic flies were generated by injecting yw embryos with pUAST-usp8-DN plasmid (BestGene, Inc.).…”

Section: Fly Strainsmentioning

confidence: 99%

See 1 more Smart Citation

CLOCK deubiquitylation by USP8 inhibits CLK/CYC transcription in Drosophila

Luo

Tang

et al. 2012

Genes Dev.

View full text Add to dashboard Cite

A conserved transcriptional feedback loop underlies animal circadian rhythms. In Drosophila, the transcription factors CLOCK (CLK) and CYCLE (CYC) activate the transcription of direct target genes like period (per) and timeless (tim). They encode the proteins PER and TIM, respectively, which repress CLK/CYC activity. Previous work indicates that repression is due to a direct PER-CLK/CYC interaction as well as CLK/CYC phosphorylation. We describe here the role of ubiquitin-specific protease 8 (USP8) in circadian transcriptional repression as well as the importance of CLK ubiquitylation in CLK/CYC transcription activity. usp8 loss of function (RNAi) or expression of a dominant-negative form of the protein (USP8-DN) enhances CLK/CYC transcriptional activity and alters fly locomotor activity rhythms. Clock protein and mRNA molecular oscillations are virtually absent within circadian neurons of USP8-DN flies. Furthermore, CLK ubiquitylation cycles robustly in wild-type flies and peaks coincident with maximal CLK/CYC transcription. As USP8 interacts with CLK and expression of USP8-DN increases CLK ubiquitylation, the data indicate that USP8 deubiquitylates CLK, which down-regulates CLK/CYC transcriptional activity. Taken together with the facts that usp8 mRNA cycles and that its transcription is activated directly by CLK/CYC, USP8, like PER and TIM, contributes to the transcriptional feedback loop cycle that underlies circadian rhythms.

show abstract

Section: Usp8 Is Important For Locomotor Activity Rhythms In Vivomentioning

confidence: 99%

Section: Fly Strainsmentioning

confidence: 99%

CLOCK deubiquitylation by USP8 inhibits CLK/CYC transcription in Drosophila

Luo

Tang

et al. 2012

Genes Dev.

View full text Add to dashboard Cite

show abstract

“…Normal flies exhibit two daily peaks of activity: a morning peak that corresponds to lights on and an evening peak around the time of lights off. After entrainment, these patterns are retained under DD conditions (Hamblen-Coyle et al 1992;Renn et al 1999;Bahn et al 2009), providing a 23-to 24-hr circadian cycle period. Results obtained for a variety of genotypes are presented in Table 1.…”

Section: Drosophila Crmp Gene 1233mentioning

confidence: 99%

“…The number of PDF--positive neurons, including both I--LNvs and s--LNvs neuronal groups in the brain, and their projection patterns appeared normal in the two mutant strains. Whole--mount anti--PDF--immunohistochemistry was performed in dissected adult brains, as described in BAHN et al 2009. A) wildtype (isoCJ1 strain) animals (10 brains examined); B) CRMP supK1 animals (11 brains examined); C) CRMP supIa1 animals (10 brains examined).…”

Section: P{ey--gal4}/p{uas--sggb}; Crmp +/Supk1mentioning

confidence: 99%

Divergent Functions Through Alternative Splicing: The Drosophila CRMP Gene in Pyrimidine Metabolism, Brain, and Behavior

et al. 2012

Self Cite

View full text Add to dashboard Cite

DHP and CRMP proteins comprise a family of structurally similar proteins that perform divergent functions, DHP in pyrimidine catabolism in most organisms and CRMP in neuronal dynamics in animals. In vertebrates, one DHP and five CRMP proteins are products of six genes; however, Drosophila melanogaster has a single CRMP gene that encodes one DHP and one CRMP protein through tissue-specific, alternative splicing of a pair of paralogous exons. The proteins derived from the fly gene are identical over 90% of their lengths, suggesting that unique, novel functions of these proteins derive from the segment corresponding to the paralogous exons. Functional homologies of the Drosophila and mammalian CRMP proteins are revealed by several types of evidence. Loss-offunction CRMP mutation modifies both Ras and Rac misexpression phenotypes during fly eye development in a manner that is consistent with the roles of CRMP in Ras and Rac signaling pathways in mammalian neurons. In both mice and flies, CRMP mutation impairs learning and memory. CRMP mutant flies are defective in circadian activity rhythm. Thus, DHP and CRMP proteins are derived by different processes in flies (tissue-specific, alternative splicing of paralogous exons of a single gene) and vertebrates (tissue-specific expression of different genes), indicating that diverse genetic mechanisms have mediated the evolution of this protein family in animals. BACTERIAL hydantoinases, dihydropyrimidinase (DHP), and collapsin response mediator proteins (CRMPs) comprise a family of proteins, which exhibit highly conserved structures, yet carry out divergent functions. DHP is found in most eukaryotes and catalyzes the second step of reductive pyrimidine catabolism (reviewed in Schnackerz and Dobritzch 2008). On the other hand, CRMPs appear to be limited to nervous systems of metazoans, where they mediate a variety of processes: semaphorin signal transduction in axonal growth cones, cytoskeletal dynamics, neuronal polarity, and modulation of neurotransmitter release (reviewed in Schmidt and Strittmatter 2007;Hou et al. 2008;Chi et al. 2009;Yamashita and Goshima 2012).All members of the DHP/CRMP family function in vivo as homotetramers and, possibly, heterotetramers (Wang and Strittmatter 1997;Deo et al. 2004); their resolved structures are extraordinarily similar (Abendroth et al. 2002a,b;Xu et al. 2003;Deo et al. 2004;Lohkamp et al. 2006;Stenmark et al. 2007). A key functional distinction among these proteins is that DHPs are zinc-coupled dihydropyrimidine hydrolases, whereas no comparable hydrolase activity has been shown for vertebrate CRMPs, which lack one or more essential zinc-binding site residues found in DHP proteins (Hamajima et al. 1998;Wang and Strittmatter 1997;Takemoto et al. 2000).Vertebrate CRMPs mediate a variety of neuronal growth cone dynamics through SEMA3A/NP1/PlexA signal transduction and perhaps other signaling pathways (reviewed in Schmidt and Strittmatter 2007;Hou et al. 2008;Yamashita and Goshima 2012). The roles of CRMP in these pathways ar...

show abstract

“…Several other insect species have been 72 found to show unimodal activity pattern, and thus their seasonal time measuring cannot be based 73 on above-mentioned system. For example housefly Musca domestica (Helfrich et al, 1985) and 74 some D. virilis group species (Bahn et al, 2009;Kauranen et al, 2012) show only evening 75 activity peak, while D. ananassae shows only morning activity peak (Joshi, 1999). 76…”

mentioning

confidence: 99%