Comparative analysis of outer membrane vesicles from uropathogenic Escherichia coli reveal the role of aromatic amino acids synthesis proteins in motility

Liu, LiangZhe; Law, Carmen O. K.; Nie, Qichang; Pham, Hoa; Ma, Hua; Zhang, Liang; Ho, Pak Leung; Lau, Terrence Chi-Kong

doi:10.1016/j.ijmm.2023.151573

Cited by 2 publications

(2 citation statements)

References 52 publications

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“…Even though the same amount of EVs (8 μg) was consistently analyzed across replicates, the raw data reveals variations in the number of proteins identified. As reported in prior publications 43 , 44 , these variations could arise from differences in EV production yields and EV size. Therefore, apart from sharing a conserved core EV proteome, it is suggested that factors beyond the mere presence of a protein in the overall proteome influence the determination of strain-specific EV protein content.…”

Section: Discussionmentioning

confidence: 61%

New insights into the role of Cutibacterium acnes-derived extracellular vesicles in inflammatory skin disorders

Cros,

Mir-Pedrol,

Toloza

et al. 2023

Sci Rep

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Cutibacterium acnes (C. acnes) is one of the most prevalent bacteria that forms the human skin microbiota. Specific phylotypes of C. acnes have been associated with the development of acne vulgaris, while other phylotypes have been linked to healthy skin. In this scenario, bacterial extracellular vesicles (EVs) play a role in the interkingdom communication role with the human host. The purpose of this study was to examine the impact of EVs generated by various phylotypes of C. acnes on inflammation and sebum production using different in vitro skin cell types. The main findings of this study reveal that the proteomic profile of the cargo embodied in the EVs reflects distinct characteristics of the different C. acnes phylotypes in terms of life cycle, survival, and virulence. The in vitro skin cell types showed an extended pro-inflammatory modulation of SLST A1 EVs consistently triggering the activation of the inflammation-related factors IL-8, IL-6, TNFα and GM-CSF, in comparison to SLST H1 and SLST H2. Additionally, an acne-prone skin model utilizing PCi-SEB and arachidonic acid as a sebum inducer, was employed to investigate the impact of C. acnes EVs on sebum regulation. Our findings indicated that all three types of EVs significantly inhibited sebum production after a 24-h treatment period, with SLST H1 EVs exhibiting the most pronounced inhibitory effect when compared to the positive control. The results of this study highlight the protective nature of C. acnes SLST H1 EVs and their potential use as a natural treatment option for alleviating symptoms associated with inflammation and oily skin.

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Section: Discussionmentioning

confidence: 61%

New insights into the role of Cutibacterium acnes-derived extracellular vesicles in inflammatory skin disorders

Cros,

Mir-Pedrol,

Toloza

et al. 2023

Sci Rep

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“…OMVs can play the role of cargos. For example, it has recently been shown that UPEC OMVs can contain and transport the enzyme AroB, which is responsible for the synthesis of aromatic amino acids, thereby increasing the motility of recipient bacteria [45]. They can also increase the secretion of toxins such as CNF1 or CDT [10,46].…”

Section: A Role Of Omvs In Urosepsismentioning

confidence: 99%

HlyF, an underestimated virulence factor of uropathogenicEscherichia coli

Chagneau

Payros

Goman

et al. 2023

Preprint

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Urinary tract infections (UTIs) are predominantly caused by uropathogenicEscherichia coli(UPEC). By analysing a representative collection of UPEC strains from community-acquired infections, we showed that 20 % of these strains had the ability to produce the protein HlyF. ThesehlyF+ UPEC strains were the most virulent, mostly responsible for pyelonephritis, often with bloodstream infections. Using a mouse model of UTI, we showed that HlyF was associated with the ability of UPEC to develop a urosepsis, with the presence of bacteria in the spleen and an exacerbated inflammatory response. In contrast to archetypical UPEC strains,hlyF+ UPEC strains are not restricted to phylogroup B2 and harbor a specific repertoire of virulence factors reflecting the fact that HlyF is encoded by conjugative ColV-like plasmids. These plasmids also carry antimicrobial resistance genes, which may facilitate their selection and spreading amongst people receiving antimicrobial therapy. Overall, our data suggest that HlyF is a virulence factor in UPEC and spreading of ColV-like plasmids encodinghlyFwarrants further investigation.

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Comparative analysis of outer membrane vesicles from uropathogenic Escherichia coli reveal the role of aromatic amino acids synthesis proteins in motility

Cited by 2 publications

References 52 publications

New insights into the role of Cutibacterium acnes-derived extracellular vesicles in inflammatory skin disorders

New insights into the role of Cutibacterium acnes-derived extracellular vesicles in inflammatory skin disorders

HlyF, an underestimated virulence factor of uropathogenicEscherichia coli

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