Comparative analysis of metabolic proteome variation in ascorbate-primed and unprimed wheat seeds during germination under salt stress

Fercha, Azzedine; Capriotti, Anna Laura; Caruso, Giuseppe; Cavaliere, Chiara; Samperi, Roberto; Stampachiacchiere, Serena; Laganà, Aldo

doi:10.1016/j.jprot.2014.04.040

Cited by 70 publications

(43 citation statements)

References 79 publications

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“…Salt sensitivity causes both rapid osmotic phase inhibiting growth of young leaves and a slower, ionic phase accelerating senescence of mature leaves causing reduction in crop plants yield [1]. Proteome analysis is a convenient tool for testing the response of plants to abiotic stress [2, 3]. Date palm ( Phoenix dactylifera L.) can adapt to extreme drought, heat, and relatively high levels of soil salinity [4].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, proteome of plant species changes as a response to biotic “pests” [7–9] and abiotic “chemical, salt, and drought stress” [3, 10, 11]. Proteome analysis of roots and leaves revealed a synergetic responsive network under stress; roots rapidly sensed and responded to stress, after which the stress signals were transferred to leaves and both roots and leaves showed similar metabolic pathways under stress with distinct changes [12].…”

Section: Introductionmentioning

confidence: 99%

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Proteome Analysis of Date Palm (Phoenix dactyliferaL.) under Severe Drought and Salt Stress

Rabey

Al-Malki

Abulnaja

2016

International Journal of Genomics

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Date palm cultivars differently tolerate salinity and drought stress. This study was carried out to study the response of date palm to severe salinity and drought based on leaf proteome analysis. Eighteen-month-old date palm plants were subjected to severe salt (48 g/L NaCl) and drought (82.5 g/L PEG or no irrigation) conditions for one month. Using a protein 2D electrophoresis method, 55 protein spots were analyzed using mass spectrometry. ATP synthase CF1 alpha chains were significantly upregulated under all three stress conditions. Changes in the abundance of RubisCO activase and one of the RubisCO fragments were significant in the same spots only for salt stress and drought stress with no irrigation, and oxygen-evolving enhancer protein 2 was changed in different spots. Transketolase was significantly changed only in drought stress with PEG. The expression of salt and drought stress genes of the chosen protein spots was either overexpressed or downexpressed as revealed by the high or low protein abundance, respectively. In addition, all drought tolerance genes due to no irrigation were downregulated. In conclusion, the proteome analysis of date palm under salinity and drought conditions indicated that both salinity and drought tolerance genes were differentially expressed resulting in high or low protein abundance of the chosen protein spots as a result of exposure to drought and salinity stress condition.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proteome Analysis of Date Palm (Phoenix dactyliferaL.) under Severe Drought and Salt Stress

Rabey

Al-Malki

Abulnaja

2016

International Journal of Genomics

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“…Contrarily, Azooz et al [28] observed the expression of new protein bands after priming fava bean seeds with 100 mg/L of ascorbic acid prior to their germination under salt stress. Similarly, Fercha et al [29] reported changes in the abundance of metabolic proteins in wheat seeds elicited with 0.5 mmol/L of ascorbic acid during germination under salt stress. These findings suggest that the influence of elicitation on sprout protein composition is particularly pronounced under additional stress conditions.…”

Section: Effect Of Elicitation On Protein Content and Profile Of Spromentioning

confidence: 86%

Impact of Elicitation on Antioxidant and Potential Antihypertensive Properties of Lentil Sprouts

Peñas

Limón

Martinez-Villaluenga

et al. 2015

Plant Foods Hum Nutr

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The aim of this study was to investigate the application of elicitors (500 μM ascorbic acid, 50 μM folic acid, 5 mM glutamic acid and 50 ppm chitosan in 5 mM glutamic acid) during lentil germination up to 8 days as a strategy to increase germination rate and to enhance the accumulation of γ-aminobutyric acid (GABA) and phenolic compounds. The effect of elicitation on the protein profile and antioxidant and angiotensin I converting enzyme (ACE) inhibitory activities of sprouted lentils was also evaluated. The application of elicitors did not negatively affect the germination yield of lentils and no significant changes on the protein pattern of lentils germinated in the presence of elicitors were observed. Chitosan/glutamic acid increased by 1.6-fold the GABA content in lentil sprouts, whilst ascorbic and folic acids as well as chitosan/glutamic acid were highly effective to enhance the total content of phenolic compounds and the antioxidant activity of sprouted lentils. All elicited lentil sprouts showed ability to inhibit ACE activity (IC50: 9.5-11.9 μg peptides/mL). Therefore, elicitation can be considered a promising approach to improve the content of compounds with antioxidant and potential antihypertensive activities in lentil sprouts.

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“…The remaining TCA cycle proteins show differential changes in abundance under salinity stress probably because of the influence of salt concentration, duration of exposure to salinity stress, different developmental stage of different organs, and different protein isoforms. The abundance of ACO, for example, was reduced in the roots of 18‐day‐old arabidopsis after exposure to salinity for 6 to 48 hr (Jiang, Yang, Harris, & Deyholos, ), whereas there were increases in ACO abundance observed in the root and embryo of salt‐tolerant wheat that was treated with salt from the beginning of growth (Fercha et al, ; Jacoby et al, ). The abundance of SDH1 was also found to differentially change between male and female leaves of Populus cathayana under salinity stress (Chen et al, ), and changes of abundance of MDH under salinity stress in Amaranthus cruentus show isoform‐dependent responses (Huerta‐Ocampo et al, ).…”

Section: Tca Cycle Responses To Salinity Stressmentioning

confidence: 99%

Connecting salt stress signalling pathways with salinity‐induced changes in mitochondrial metabolic processes inC3 plants

Che-Othman

Millar

Taylor

2017

Plant Cell & Environment

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Salinity exerts a severe detrimental effect on crop yields globally. Growth of plants in saline soils results in physiological stress, which disrupts the essential biochemical processes of respiration, photosynthesis, and transpiration. Understanding the molecular responses of plants exposed to salinity stress can inform future strategies to reduce agricultural losses due to salinity; however, it is imperative that signalling and functional response processes are connected to tailor these strategies. Previous research has revealed the important role that plant mitochondria play in the salinity response of plants. Review of this literature shows that 2 biochemical processes required for respiratory function are affected under salinity stress: the tricarboxylic acid cycle and the transport of metabolites across the inner mitochondrial membrane. However, the mechanisms by which components of these processes are affected or react to salinity stress are still far from understood. Here, we examine recent findings on the signal transduction pathways that lead to adaptive responses of plants to salinity and discuss how they can be involved in and be affected by modulation of the machinery of energy metabolism with attention to the role of the tricarboxylic acid cycle enzymes and mitochondrial membrane transporters in this process. Saline landscapes occur naturally, and many halophytes (salt tolerant plants) survive and complete their life cycle in these environments (Flowers & Colmer, 2015). Human activity including intense irrigation and land clearing can also cause soil salinity, as these activities lead to changes in the soil water table, which can contain high salt concentrations (Rengasamy, 2006). These agricultural practices result in a rising water table, which liberates soil solutes bringing them to the surface, creating salinized areas in previously productive land (Rengasamy, 2006). This severely limits crop production in these areas as typically elite high yielding crop varieties cannot tolerate these high salt concentrations (Roy, Negrão, & Tester, 2014).Although the salinization of soils has and is increasingly limiting crop production in many areas of the world (Sha Valli Khan, Nagamallaiah, Dhanunjay, Sergeant, & Hausman, 2014), reliable measures of salt-affected land are very difficult to obtain because salinity varies both spatially and temporally. Nevertheless, a number of studies have attempted to estimate the extent of soil salinity.According to the Food and Agriculture Organisation of the United Nations, it is estimated that approximately 20% of all global irrigated land has become salt-affected and this percentage is expected to continue to increase over time (FAO, 2009;Parihar, Singh, Singh, Singh, & Prasad, 2014). It was further estimated that this salinization of irrigated --------------------------------------------------This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium...

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Comparative analysis of metabolic proteome variation in ascorbate-primed and unprimed wheat seeds during germination under salt stress

Cited by 70 publications

References 79 publications

Proteome Analysis of Date Palm (Phoenix dactyliferaL.) under Severe Drought and Salt Stress

Proteome Analysis of Date Palm (Phoenix dactyliferaL.) under Severe Drought and Salt Stress

Impact of Elicitation on Antioxidant and Potential Antihypertensive Properties of Lentil Sprouts

Connecting salt stress signalling pathways with salinity‐induced changes in mitochondrial metabolic processes inC3 plants

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