Comparative Analysis of Metabolic Differences of Jersey Cattle in Different High-Altitude Areas

Kong, Zhiwei; Li, Bin; Zhou, Changlin; He, Q.; Zheng, Yuzhong; Tan, Zhiliang

doi:10.3389/fvets.2021.713913

Cited by 8 publications

(8 citation statements)

References 45 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacteria play a key role in many types of feed biopolymer fermentation and degradation processes (Bickhart and Weimer, 2018), and fecal samples mostly represent the distal portion of the gut microbiotathe (de Oliveira et al, 2013). Recent studies have reported the high-altitude adaptability of various animals such as cattle (Kong et al, 2021), yak (Ayalew et al, 2021), and rats (Murray, 2016). Recently, alterations in the gastrointestinal microbiota due to altitude changes were investigated in yak (Liu et al, 2021), sheep (Holman et al, 2019), and pigs (Zeng et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Effects of altitude on the gut microbiome and metabolomics of Sanhe heifers

Zhang

Wang²,

Cao³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionExtreme environments at high altitudes pose a significant physiological challenge to animals. We evaluated the gut microbiome and fecal metabolism in Sanhe heifers from different altitudes.MethodsTwenty Sanhe heifers (body weight: 334.82 ± 13.22 kg, 15-month-old) selected from two regions of China: the Xiertala Cattle Breeding Farm in Hulunbeier, Inner Mongolia [119°57′ E, 47°17′ N; approximately 700 m altitude, low altitude (LA)] and Zhizhao Dairy Cow Farm in Lhasa, Tibet [91°06′ E, 29°36′ N; approximately 3,650 m altitude, high altitude (HA)], were used in this study. Fecal samples were collected and differences in the gut microbiota and metabolomics of Sanhe heifers were determined using 16S rRNA gene sequencing and metabolome analysis.Results and discussionThe results showed that altitude did not significantly affect the concentrations of fecal volatile fatty acids, including acetate, propionate, butyrate, and total volatile fatty acids (p > 0.05). However, 16S rRNA gene sequencing showed that altitude significantly affected gut microbial composition. Principal coordinate analysis based on Bray–Curtis dissimilarity analysis revealed a significant difference between the two groups (p = 0.001). At the family level, the relative abundances of Peptostreptococcaceae, Christensenellaceae, Erysipelotrichaceae, and Family_XIII were significantly lower (p < 0.05) in LA heifers than in HA heifers. In addition, the relative abundances of Lachnospiraceae, Domibacillus, Bacteroidales_S24-7_group, Bacteroidales_RF16_group, Porphyromonadaceae, and Spirochaetaceae were significantly higher in HA heifers than in LA heifers (p < 0.05). Metabolomic analysis revealed the enrichment of 10 metabolic pathways, including organismal systems, metabolism, environmental information processing, genetic information processing, and disease induction. The genera Romboutsia, Paeniclostridium, and g_unclassified_f_Lachnospiraceae were strongly associated with the 28 differential metabolites. This study is the first to analyze the differences in the gut microbiome and metabolome of Sanhe heifers reared at different altitudes and provides insights into the adaptation mechanism of Sanhe heifers to high-altitude areas.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of altitude on the gut microbiome and metabolomics of Sanhe heifers

Zhang

Wang²,

Cao³

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…In previous studies, we found that Jersey cattle showed adaptations to high altitude areas at both the miRNA and proteome levels ( Kong et al, 2019 ), illustrating adaptive mechanisms through up-regulation of amino acid metabolism and sphingolipid metabolism ( Kong et al, 2021 ). Zhang et al (2022) found that rumen fermentation and bacteria characteristic of highland Sanhe heifers had a lower acetate-to-propionate ratio as alternative hydrogen electron acceptors for CH 4 mitigation, a lower relative abundance of Actinobacteria, and higher relative abundance of Spirochaetae than that in rumen of lowland heifers.…”

Section: Overview Of Introduced Ruminants On the Qinghai–tibetan Plateaumentioning

confidence: 83%

Rumen microbiota of indigenous and introduced ruminants and their adaptation to the Qinghai–Tibetan plateau

Jia²,

Wen³

et al. 2022

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

The grassland in the Qinghai–Tibetan plateau provide habitat for many indigenous and introduced ruminants which perform important ecological functions that impact the whole Qinghai–Tibetan plateau ecosystem. These indigenous Tibetan ruminants have evolved several adaptive traits to withstand the severe environmental conditions, especially cold, low oxygen partial pressure, high altitude, strong UV radiation, and poor forage availability on the alpine rangelands. Despite the challenges to husbandry associated with the need for enhanced adaptation, several domesticated ruminants have also been successfully introduced to the alpine pasture regions to survive in the harsh environment. For ruminants, these challenging conditions affect not only the host, but also their commensal microbiota, especially the diversity and composition of the rumen microbiota; multiple studies have described tripartite interactions among host-environment-rumen microbiota. Thus, there are significant benefits to understanding the role of rumen microbiota in the indigenous and introduced ruminants of the Qinghai–Tibetan plateau, which has co-evolved with the host to ensure the availability of specific metabolic functions required for host survival, health, growth, and development. In this report, we systemically reviewed the dynamics of rumen microbiota in both indigenous and introduced ruminants (including gut microbiota of wild ruminants) as well as their structure, functions, and interactions with changing environmental conditions, especially low food availability, that enable survival at high altitudes. We summarized that three predominant driving factors including increased VFA production, enhanced fiber degradation, and lower methane production as indicators of higher efficiency energy harvest and nutrient utilization by microbiota that can sustain the host during nutrient deficit. These cumulative studies suggested alteration of rumen microbiota structure and functional taxa with genes that encode cellulolytic enzymes to potentially enhance nutrient and energy harvesting in response to low quality and quantity forage and cold environment. Future progress toward understanding ruminant adaptation to high altitudes will require the integration of phenotypic data with multi-omics analyses to identify host-microbiota co-evolutionary adaptations enabling survival on the Qinghai–Tibetan plateau.

show abstract

“…The Jersey cow, which originated in the United Kingdom, has been widely introduced in various areas including Tibet due to its strong disease resistance, rough feeding tolerance, high feeding utilization rate, and high milk fat content (which is the origin of butter) ( Kong et al, 2021 ). Liu et al (2022) found that Jersey cows were the species that had the best adaptation out of all the breeds that had been introduced to high-altitude regions.…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, the composition of the bacterial community in the rumen can be examined through 16S rRNA gene amplicon sequences ( Pu et al, 2023 ). Metabolomics based on mass spectrometry (MS) is a method to study the overall changes of small-molecule metabolites, reflecting the physiological activities in organisms ( Kong et al, 2021 ). Therefore, we used 16S rRNA and metabolomics to study which feeding strategy is appropriate for Jersey cows in Tibetan Plateau during the cold season when forage resources are in short supply.…”

Section: Introductionmentioning

confidence: 99%

From the perspective of rumen microbiome and host metabolome, revealing the effects of feeding strategies on Jersey Cows on the Tibetan Plateau

Yuan,

Wang,

Pan

et al. 2023

PeerJ

View full text Add to dashboard Cite

Background Previous studies have discussed the effects of grazing and house feeding on yaks during the cold season when forage is in short supply, but there is limited information on the effects of these feeding strategies on Jersey cows introduced to the Tibetan Plateau. The objective of this study was to use genomics and metabolomics analyses to examine changes in rumen microbiology and organism metabolism of Jersey cows with different feeding strategies. Methods We selected 12 Jersey cows with similar body conditions and kept them for 60 days under grazing (n = 6) and house-feeding (n = 6) conditions. At the end of the experiment, samples of rumen fluid and serum were collected from Jersey cows that had been fed using different feeding strategies. The samples were analyzed for rumen fermentation parameters, rumen bacterial communities, serum antioxidant and immunological indices, and serum metabolomics. The results of the study were examined to find appropriate feeding strategies for Jersey cows during the cold season on the Tibetan plateau. Results The results of rumen fermentation parameters showed that concentrations of acetic acid, propionic acid, and ammonia nitrogen in the house-feeding group (Group B) were significantly higher than in the grazing group (Group G) (P < 0.05). In terms of the rumen bacterial community 16S rRNA gene, the Chao1 index was significantly higher in Group G than in Group B (P = 0.038), while observed species, Shannon and Simpson indices were not significantly different from the above-mentioned groups (P > 0.05). Beta diversity analysis revealed no significant differences in the composition of the rumen microbiota between the two groups. Analysis of serum antioxidant and immune indices showed no significant differences in total antioxidant capacity between Group G and Group B (P > 0.05), while IL-6, Ig-M , and TNF-α were significantly higher in Group G than in Group B (P < 0.05). LC–MS metabolomics analysis of serum showed that a total of 149 major serum differential metabolites were found in Group G and Group B. The differential metabolites were enriched in the metabolic pathways of biosynthesis of amino acids, protein digestion and absorption, ABC transporters, aminoacyl-tRNA biosynthesis, mineral absorption, and biosynthesis of unsaturated fatty acids. These data suggest that the house-feeding strategy is more beneficial to improve the physiological state of Jersey cows on the Tibetan Plateau during the cold season when forages are in short supply.

show abstract

Comparative Analysis of Metabolic Differences of Jersey Cattle in Different High-Altitude Areas

Cited by 8 publications

References 45 publications

Effects of altitude on the gut microbiome and metabolomics of Sanhe heifers

Effects of altitude on the gut microbiome and metabolomics of Sanhe heifers

Rumen microbiota of indigenous and introduced ruminants and their adaptation to the Qinghai–Tibetan plateau

From the perspective of rumen microbiome and host metabolome, revealing the effects of feeding strategies on Jersey Cows on the Tibetan Plateau

Contact Info

Product

Resources

About