Effects of altitude on the gut microbiome and metabolomics of Sanhe heifers

Zhang, Xinyu; Wang, Wei; Cao, Zhijun; Yang, Hongjian; Wang, Yajing; Li, Shengli

doi:10.3389/fmicb.2023.1076011

Cited by 5 publications

(3 citation statements)

References 54 publications

(49 reference statements)

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“…This study explored the ruminal microbes and found that there were significant differences at different taxonomic levels. Firmicutes and Bacteroidetes, which mainly participate in the absorption and digestion of carbohydrates and proteins, had the highest abundance at the phylum level, consistent with a previous study [ 30 , 31 ]. Tenericutes, Kiritimatiellaeota, and Elusimicrobia were the three significantly different microbial phyla, which had greater relative abundance in group C. Tenericutes and Elusimicrobia are minor members, and their role in rumen is uncertain [ 32 , 33 ].…”

Section: Discussionsupporting

confidence: 92%

Drinking Warm Water Promotes Performance by Regulating Ruminal Microbial Composition and Serum Metabolites in Yak Calves

et al. 2023

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Yaks live in the harsh environment of the Qinghai–Tibet Plateau, and the cold climate causes lower growth efficiency. The aim of this experiment was to explore the effects of drinking warm water on the growth performance in yak calves and investigate the underlying physiological mechanisms. A total of 24 Datong yak calves were selected and randomly assigned into the cold water group (group C, water temperature around 0–10 °C without any heating; 58.03 ± 3.111 kg) and the warm water group (group W, water constantly heated at 2 °C; 59.62 ± 2.771 kg). After the 60-day experiment, body weight was measured, and rumen fluid and blood serum samples were collected for analysis. The results show that the body weight and average daily gain of yaks that drank warm water were higher compared to those that drank cold water (p < 0.05). The acetic, propionic, isobutyric, valeric, and isovaleric acid concentrations were higher in group W than in group C (p < 0.05). Additionally, warm water changed the ruminal microbes at different levels. At the phylum level, the relative abundance of Tenericutes, Kiritimatiellaeota, and Elusimicrobiota was higher in group C (p < 0.05). At the genus level, three genera were increased by warm water, including Ruminococcoides and Eubacteriales Family XIII. Incertae Sedis, and 12 genera were decreased, including Ruminococcus (p < 0.05). At the species level, unclassified Prevotellaceae and Ruminococcoides bili were increased by warm water compared to cold water (p < 0.05). According to the metabolomics results, metabolites, including valine, isoleucine, PC (15:0/22:2(13Z,16Z)), and LysoPC (18:0/0:0), were increased in the warm water group compared to the cold water group (p < 0.05), and were enriched in glycerophospholipid and amino acid metabolism pathways. This study analyzed the differences in ruminal microbes and metabolomes of yak calves provided with water at different temperatures and revealed the potential mechanism for better performance promoted by warm drinking water.

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Section: Discussionsupporting

confidence: 92%

Drinking Warm Water Promotes Performance by Regulating Ruminal Microbial Composition and Serum Metabolites in Yak Calves

et al. 2023

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“…Altitude change has emerged as a major driving factor of fluctuations in the gut microbiota, influencing both bacterial composition and function. Different altitudes have been found to induce varying changes in the composition and abundance of the gut microbiota ( Zhang et al, 2023a ). A prior study investigating the characteristics of the intestinal microbiota of six geographical populations of Macaca mulatta in China found that M. mulatta populations in Tibet exhibited the highest intestinal microbiota diversity and possessed the highest number of unique microbiota ( Zhao et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Impact of high-altitude acclimatization and de-acclimatization on the intestinal microbiota of rats in a natural high-altitude environment

Hao,

Niu,

Zhao

et al. 2024

Front. Microbiol.

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IntroductionIntestinal microorganisms play an important role in the health of both humans and animals, with their composition being influenced by changes in the host’s environment.MethodsWe evaluated the longitudinal changes in the fecal microbial community of rats at different altitudes across various time points. Rats were airlifted to high altitude (3,650 m) and acclimatized for 42 days (HAC), before being by airlifted back to low altitude (500 m) and de-acclimatized for 28 days (HADA); meanwhile, the control group included rats living at low altitude (500 m; LA). We investigated changes in the gut microbiota at 12 time points during high-altitude acclimatization and de-acclimatization, employing 16S rRNA gene sequencing technology alongside physiological indices, such as weight and daily autonomous activity time.ResultsA significant increase in the Chao1 index was observed on day 14 in the HAC and HADA groups compared to that in the LA group, indicating clear differences in species richness. Moreover, the principal coordinate analysis revealed that the bacterial community structures of HAC and HADA differed from those in LA. Long-term high-altitude acclimatization and de- acclimatization resulted in the reduced abundance of the probiotic Lactobacillus. Altitude and age significantly influenced intestinal microbiota composition, with changes in ambient oxygen content and atmospheric partial pressure being considered key causal factors of altitude-dependent alterations in microbiota composition. High-altitude may be linked to an increase in anaerobic bacterial abundance and a decrease in non-anaerobic bacterial abundance.DiscussionIn this study, the hypobaric hypoxic conditions at high-altitude increased the abundance of anaerobes, while reducing the abundance of probiotics; these changes in bacterial community structure may, ultimately, affect host health. Overall, gaining a comprehensive understanding of the intestinal microbiota alterations during high-altitude acclimatization and de-acclimatization is essential for the development of effective prevention and treatment strategies to better protect the health of individuals traveling between high- and low-altitude areas.

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“…In the same body weight groups (HOB vs. LOB, HN vs. LN), the main dominant bacterium was Romboutsia , which was mainly associated with the metabolism of nucleotide and amino acid compounds. The analysis of the effect of altitude on Sanhe heifers' gut microbiota and metabolism found that Romboutsia was associated with the metabolism of purine pyrimidines and amino acids (Zhang et al, 2023 ). Another study found that some potential probiotics, including Christensenellaceae_R-7_group, Ruminococcus_1, Romboutsia, Alloprevotella, E. coprostanoligenes , and Clostridium , were enriched in the rumen of high-altitude yaks.…”

Section: Discussionmentioning

confidence: 99%

How hypoxia affects microbiota metabolism in mice

Ailizire,

Wang,

et al. 2023

Front. Microbiol.

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ObjectiveTo investigate the relationship between gut microbiota and the fecal metabolites of hypoxic environments in mice.MethodsHigh-fat diet-induced obese mice (n = 20) and normal diet-fed mice (n = 20) were randomly divided into four groups: high altitude obese group (HOB), high altitude normal weight group (HN), low altitude obese group LOB (LOB), and low altitude normal weight group (LN). Fecal samples from each group were 16S rRNA gene sequenced, and five samples from each of the four groups above were selected for non-targeted fecal metabolomics analysis using liquid chromatography-mass spectrometry. The relationship between gut microbiota and fecal metabolites was analyzed using SIMCA 14.1, MetaboAnalyst 5.0 and R 4.1.11.Results(A) Body weight was significantly lower in the hypoxic obesity group than in the normoxic obesity group. (B) Differences in α-diversity and β-diversity were found in the fecal gut microbiota of mice of different body weights and altitude, and the diversity of gut microbiota was higher in the normal group than in the obese group; the results of the comparison between the two groups showed that Faecalibaculum, Romboutsia, Lactobacillus, and A2 were associated with obesity; Romboutsia was associated with hypoxia. (C) The metabolic profiles of fecal metabolites differed between groups: gut microbiota were associated with nucleotide and amino acid metabolism in the same body groups, while gut microbiota were associated with lipid and amino acid metabolism in the same oxygen concentration groups.Conclusion(a) Gut microbiota diversity was reduced in obese groups. Romboutsia was the dominant microbiota in the hypoxia group. (b) Gut microbiota were associated with nucleotide and amino acid metabolism in the same body weight groups, while they were associated with lipid and amino acid metabolism in the same altitude groups.

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Effects of altitude on the gut microbiome and metabolomics of Sanhe heifers

Cited by 5 publications

References 54 publications

Drinking Warm Water Promotes Performance by Regulating Ruminal Microbial Composition and Serum Metabolites in Yak Calves

Drinking Warm Water Promotes Performance by Regulating Ruminal Microbial Composition and Serum Metabolites in Yak Calves

Impact of high-altitude acclimatization and de-acclimatization on the intestinal microbiota of rats in a natural high-altitude environment

How hypoxia affects microbiota metabolism in mice

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