Comparative analysis of major biological aspects of human hepatoma in culture

Bagnarelli, Patrizia; Festa, A; Testa, I; Champ, Brigitte; Clementi, Massimo; Caroloni, G.

doi:10.1016/b978-0-408-02732-8.50017-1

Cited by 1 publication

(2 citation statements)

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“…8). This mitogenic response to EGF shares homologies with that of most differentiated HCC cell lines (12). It is in line with the normal expression of the membrane EGF-Rs observed in these cells (data not shown).…”

Section: Resultssupporting

confidence: 83%

“…AST, ALT, glucose-6-phosphatase and hexokinase isoenzyme activities were examined as described elsewhere (19). The assay for epidermal growth factor (EGF) membrane receptor (EGF-R) was performed as described previously (12,13). Finally, two MAbs to antigens expressed in HCC cell lines (IB1 and 9B2) were kindly supplied by Dr. C. Chang and used according to the method described in the original paper (20) in an indirect immunofluorescence test to confirm the hepatocytic origin of the PLC/AN/2 cell line.…”

Section: Patientmentioning

confidence: 99%

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Growth–Factor Independence of A New Differentiated Hepatitis B Virus Dna–Negative Human Hepatoma Cell Line

et al. 1990

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The establishment of a new, differentiated, hepatitis B virus DNA-negative, human hepatoma cell line (named PLC/AN/2) is described. Neoplastic liver tissue was obtained during hepatectomy in an HBsAg-negative man. The established cell line is negative for alpha-fetoprotein and carcinoembryonic antigen; it has retained in vitro some of the differentiated functions of normal hepatocytes. Additionally, it presents a distinctive rearrangement (translocation) at the long arm of chromosome 4. The high degree of independence from serum growth factor requirements appears to be a major in vitro characteristic of PLC/AN/2 cells, making them a suitable model system for the more precise definition of the human hepatocellular carcinoma phenotype, including mechanisms of growth control.

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Section: Resultssupporting

confidence: 83%