Growth–Factor Independence of A New Differentiated Hepatitis B Virus Dna–Negative Human Hepatoma Cell Line

Abstract

The establishment of a new, differentiated, hepatitis B virus DNA-negative, human hepatoma cell line (named PLC/AN/2) is described. Neoplastic liver tissue was obtained during hepatectomy in an HBsAg-negative man. The established cell line is negative for alpha-fetoprotein and carcinoembryonic antigen; it has retained in vitro some of the differentiated functions of normal hepatocytes. Additionally, it presents a distinctive rearrangement (translocation) at the long arm of chromosome 4. The high degree of independence from serum growth factor requirements appears to be a major in vitro characteristic of PLC/AN/2 cells, making them a suitable model system for the more precise definition of the human hepatocellular carcinoma phenotype, including mechanisms of growth control.

Establishment of cell clones with different metastatic potential from the metastatic hepatocellular carcinoma cell line MHCC97

“…As early as in the 1960s, Chen [51] establish ed the first human HCC cell line in the world. Later on several HCC cell lines were established [52][53][54][55][56][57][58] . Although most of these showed tumorigenicity when inoculated into experimental animals, rarely did they demonstrate the full potential for loco-regional and distant metastases, as seen so frequently in clinical patients.…”

Metastatic human hepatocellular carcinoma models in nude mice and cell line with metastatic potential

“…Many human HCC cell lines have been established for the studies on etiological factors of HCC, such as hepatitis B virus (HBV) [9][10][11][12][13][14] , hepatitis C virus (HCV) [15] , hemochromatosis [16] , thorotrast [17] , for the study of alpha fetoprotein (AFP) [18,19] , and for other studies [20][21][22][23][24][25][26][27][28][29][30] . Unfortunately, of the above human HCC cell lines, metastatic potential was rarely mentioned or demonstrated.For animal HCC cell lines, the establishment of such cell lines in rat [31] , in woodchuck [32][33][34] , and in chicken [35] have been reported.…”

Two new human cholangiocarcinoma cell lines and their cytogenetics and responses to growth factors, hormones, cytokines or immunologic effector cells

“…Although many human hepatocellular carcinoma (HCC) cell lines have been established and characterized (e.g., He et al. 1984;Bagnarelli et a/., 1990), only a few CC (intrahepatic bile-duct carcinoma) lines have been described in the literature (Homma rt al., 1987;Yamaguchi et al, 1985;Miyagiwa et aL, 1989;Storto et al, 1990). This may be due, in part, to the lower incidence of CC in comparison with HCC (Okuda and Nakashima, 1985) or to difficulties in the isolation of sufficient numbers of viable tumor cells from CC, which often contain dense fibrous tissue.…”