Comparative analysis of in vitro osteo/odontogenic differentiation potential of human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAP)

Odontogenic differentiation of vascular endothelial growth factor-transfected human dental pulp stem cells in vitro

“…Another study also confirmed the improved osteogenic differentiation capacity of rat BMSCs by lentivirus-mediated VEGF gene transfection (13). Due to the similarity between osteogenic and odontogenic differentiation among DPSCs (14), lentiviral vector-mediated stable transfection of the VEGF gene may be an effective strategy to improve the odontogenic differentiation capacity of DPSCs.…”

“…STRO-1 has been previously observed in pulp tissues and is regarded as a marker for stromal stem cells, recognizing the trypsin-insensitive epitope on perivascular cells (4). In the majority of previous studies, STRO-1 expression has been observed in DPSCs, with an expression level ranging between 0.46 and 26.14% (4,14,17). CD146 is a stem cell marker identified in adult bone marrow and dental pulp tissue, associated with blood vessels (18).…”

“…The multipotent differentiation potential of the cells was identified by osteogenic and adipogenic differentiation induction (14). Briefly, the cells were exposed to osteogenic medium (DMEM supplemented with 10 nmol/l dexamethasone, 10 mmol/l β-glycerophosphate, 50 µg/ml ascorbate phosphate, 10 nmol/l 1,25 dihydroxyvitamin D3 and 10% FBS) and adipogenic medium (DMEM supplemented with 1 µmol/l dexamethasone, 1 µg/ml insulin, 0.5 mmol/l 3-isobutyl-1-methylxantine and 10% FBS) for 28 days.…”

Odontogenic epithelial stem cells: hidden sources

“…68 The regeneration of non-dental tissues of critical value, such as the cornea, cardiac cells, neurons, pancreatic islets, and retina, from oral and DSCs will have favorable results in the near future because of their embryonic origin. [18][19][20][21][22][23]43 The current treatment protocols for dental defects by either the fillings with biocompatible materials or the tooth replacement with dental implants are well established with standard techniques, which are economically affordable and ethically safe. Clinical studies on human dental tissue regeneration should target acceptable and easy procedures for application in regenerative medicine with nano-structured materials as reported by Mitsiadis et al 69 and Mitsiadis and Papagerakis.…”

“…3,9,[13][14][15][16][17] Many reports have described the multi-potentiality and possible applications of mesenchymal DSCs in dental and non-dental tissue regeneration. [18][19][20][21][22][23] [18][19][20][21][22][23][24] Their uniqueness of development from CNCCs explains their multilineage differentiation characteristics.…”