Comparative analysis of imaging configurations and objectives for Fourier microscopy

Kurvits, Jonathan A.; Jiang, Mingming; Zia, Rashid

doi:10.1364/josaa.32.002082

Cited by 102 publications

(92 citation statements)

References 44 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…35), and an electron-multiplying charge-coupled device (EMCCD) camera (Andor iXon Ultra 897). A custom-made polarization filter can be optionally inserted to limit collection polarization to a particular orientation with respect to the wire.…”

Section: Methodsmentioning

confidence: 99%

Super-resolution imaging of light–matter interactions near single semiconductor nanowires

et al. 2016

View full text Add to dashboard Cite

Nanophotonics is becoming invaluable for an expanding range of applications, from controlling the spontaneous emission rate and the directionality of quantum emitters, to reducing material requirements of solar cells by an order of magnitude. These effects are highly dependent on the near field of the nanostructure, which constitutes the evanescent fields from propagating and resonant localized modes. Although the interactions between quantum emitters and nanophotonic structures are increasingly well understood theoretically, directly imaging these interactions experimentally remains challenging. Here we demonstrate a photoactivated localization microscopy-based technique to image emitter-nanostructure interactions. For a 75 nm diameter silicon nanowire, we directly observe a confluence of emission rate enhancement, directivity modification and guided mode excitation, with strong interaction at scales up to 13 times the nanowire diameter. Furthermore, through analytical modelling we distinguish the relative contribution of these effects, as well as their dependence on emitter orientation.

show abstract

Section: Methodsmentioning

confidence: 99%

Super-resolution imaging of light–matter interactions near single semiconductor nanowires

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The high NA objective is one likely contributor to these aberrations, as supported by recent studies of (global) aberrations in orientational imaging(181) and comparative modeling of Fourier microscopy configurations. (182)…”

Section: Challenges and New Developments In 3d Localizationmentioning

confidence: 99%

Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking

2017

View full text Add to dashboard Cite

Single-molecule super-resolution fluorescence microscopy and single-particle tracking are two imaging modalities that illuminate the properties of cells and materials on spatial scales down to tens of nanometers, or with dynamical information about nanoscale particle motion in the millisecond range, respectively. These methods generally use wide-field microscopes and two-dimensional camera detectors to localize molecules to much higher precision than the diffraction limit. Given the limited total photons available from each single-molecule label, both modalities require careful mathematical analysis and image processing. Much more information can be obtained about the system under study by extending to three-dimensional (3D) single-molecule localization: without this capability, visualization of structures or motions extending in the axial direction can easily be missed or confused, compromising scientific understanding. A variety of methods for obtaining both 3D super-resolution images and 3D tracking information have been devised, each with their own strengths and weaknesses. These include imaging of multiple focal planes, point-spread-function engineering, and interferometric detection. These methods may be compared based on their ability to provide accurate and precise position information of single-molecule emitters with limited photons. To successfully apply and further develop these methods, it is essential to consider many practical concerns, including the effects of optical aberrations, field-dependence in the imaging system, fluorophore labeling density, and registration between different color channels. Selected examples of 3D super-resolution imaging and tracking are described for illustration from a variety of biological contexts and with a variety of methods, demonstrating the power of 3D localization for understanding complex systems.

show abstract

“…4 are given in Supplementary Table 1. A small amount of asymmetry was noted in the intensity of the lobes of the y-phi mask, which may be a result of small residual aberrations (e. g. coma) in the imaging system 27 (Supplementary Fig. 10 and accompanying text), or from imperfections in the mask itself.…”

Section: Methodsmentioning

confidence: 99%

Removing orientation-induced localization biases in single-molecule microscopy using a broadband metasurface mask

et al. 2016

View full text Add to dashboard Cite

Nanoscale localization of single molecules is a crucial function in several advanced microscopy techniques, including single-molecule tracking and wide-field super-resolution imaging 1. To date, a central consideration of such techniques is how to optimize the precision of molecular localization. However, as these methods continue to push toward the nanometre size scale, an increasingly important concern is the localization accuracy. In particular, single fluorescent molecules emit with an anisotropic radiation pattern of an oscillating electric dipole, which can cause significant localization biases using common estimators 2-5. Here we present the theory and experimental demonstration of a solution to this problem based on azimuthal filtering in the Fourier plane of the microscope. We do so using a high efficiency dielectric metasurface polarization/phase device composed of nanoposts with sub-wavelength spacing 6. The method is demonstrated both on fluorophores embedded in a polymer matrix, and in dL5 protein complexes that bind Malachite green 7, 8.

show abstract

Comparative analysis of imaging configurations and objectives for Fourier microscopy

Cited by 102 publications

References 44 publications

Super-resolution imaging of light–matter interactions near single semiconductor nanowires

Super-resolution imaging of light–matter interactions near single semiconductor nanowires

Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking

Removing orientation-induced localization biases in single-molecule microscopy using a broadband metasurface mask

Contact Info

Product

Resources

About