Comparative analysis of fruit ripening and associated genes in two kiwifruit cultivars (‘Sanuki Gold’ and ‘Hayward’) at various storage temperatures

Mitalo, Oscar W.; Asiche, William Olubero; Kasahara, Yuka; Tosa, Yasuaki; Tokiwa, Sumire; Ushijima, Koichiro; Nakano, Ryohei; Kubo, Yasutaka

doi:10.1016/j.postharvbio.2018.08.017

Cited by 19 publications

(19 citation statements)

References 43 publications

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“…The regulation of fruit ripening by low temperature is not unique to citrus fruit. Previous studies have also demonstrated its role, either independently or in concert with ethylene, in the regulation of fruit ripening in various species such as kiwifruit ( Mworia et al , 2012 ; Asiche et al, 2017 , 2018 ; Mitalo et al, 2018 b , 2019 a , 2019 b ), pears ( El-Sharkawy et al , 2003 ; Mitalo et al , 2019c ), and apples ( Tacken et al , 2010 ). From an ecological perspective, the primary purpose of ripening is to make fruit attractive to seed-dispersing organisms.…”

Section: Discussionmentioning

confidence: 99%

“…However, promotion of fruit ripening by low temperature has been described in various fruit species, including kiwifruit ( Kim et al , 1999 ; Mworia et al , 2012 ; Asiche et al , 2017 ; Mitalo et al , 2018 b ), European pears ( El-Sharkawy et al , 2003 ; Nham et al , 2017 ), and apples ( Tacken et al , 2010 ). Recently, transcriptome studies have suggested that low temperature-specific genes might have regulatory roles during fruit ripening in kiwifruit ( Asiche et al , 2018 ; Mitalo et al, 2018 a , 2019 a , 2019 b ) and European pears ( Mitalo et al , 2019c ).…”

Section: Introductionmentioning

confidence: 99%

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Low temperature modulates natural peel degreening in lemon fruit independently of endogenous ethylene

Mitalo

Otsuki

Okada

et al. 2020

Journal of Experimental Botany

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Peel degreening is an important aspect of fruit ripening in many citrus fruit, and previous studies have shown that it can be advanced by ethylene treatment or by low-temperature storage. However, the important regulators and pathways involved in natural peel degreening remain largely unknown. To determine how natural peel degreening is regulated in lemon fruit (Citrus limon), we studied transcriptome and physiochemical changes in the flavedo in response to ethylene treatment and low temperatures. Treatment with ethylene induced rapid peel degreening, which was strongly inhibited by the ethylene antagonist, 1-methylcyclopropene (1-MCP). Compared with 25 ºC, moderately low storage temperatures of 5–20 °C also triggered peel degreening. Surprisingly, repeated 1-MCP treatments failed to inhibit the peel degreening induced by low temperature. Transcriptome analysis revealed that low temperature and ethylene independently regulated genes associated with chlorophyll degradation, carotenoid metabolism, photosystem proteins, phytohormone biosynthesis and signalling, and transcription factors. Peel degreening of fruit on trees occurred in association with drops in ambient temperature, and it coincided with the differential expression of low temperature-regulated genes. In contrast, genes that were uniquely regulated by ethylene showed no significant expression changes during on-tree peel degreening. Based on these findings, we hypothesize that low temperature plays a prominent role in regulating natural peel degreening independently of ethylene in citrus fruit.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Low temperature modulates natural peel degreening in lemon fruit independently of endogenous ethylene

Mitalo

Otsuki

Okada

et al. 2020

Journal of Experimental Botany

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“…3 named AcßAMY2 and BAM3L ; and BAM3 . 4 named BAM3 in recent publications [43, 44]. Hopefully this new naming will address these discrepancies.…”

Section: Resultsmentioning

confidence: 99%

Copy number variants in kiwifruit ETHYLENE RESPONSE FACTOR/APETALA2 (ERF/AP2)-like genes show divergence in fruit ripening associated cold and ethylene responses in C-REPEAT/DRE BINDING FACTOR-like genes

et al. 2019

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The ETYHLENE RESPONSE FACTOR/APETALA2 ( ERF/AP2 ) transcription factors have been shown to control a wide range of developmental and environmental responses in plants. These include hormonal responses to ethylene and Abscisic Acid (ABA) as well as to cold and drought. In Actinidia chinensis (kiwifruit), ripening is unusual: although it is sometimes classed as a climacteric fruit (ethylene-associated ripening), much of fruit ripening occurs independently from autocatalytic ethylene production. Initiation of ripening appears to be strongly developmentally controlled and modulated by low temperature. In this study, fruit treated with different temperatures showed an increase in soluble sugar accumulation, and a corresponding increase in ß-AMYLASE ( BAM ) genes (predominantly BAM3 . 2 and BAM9 ) with lower temperatures. To investigate the potential role of the AP2/ERF gene family in the control of fruit ripening in kiwifruit this family was investigated further. Using the new genome annotation and further genome sequence analysis we identified 226 ERF -like genes, 10 AP2L / RAV -like genes and 32 AP2 -like genes. An RNA-seq screen from kiwifruit of different maturities, and following treatment with ethylene and temperatures between 0 and 16°C, revealed 4%, 26% and 18% of the ERF -like genes were upregulated by maturation, ethylene and cold temperatures, respectively. Focusing on the C-REPEAT/DRE BINDING FACTOR ( CBF ) cold master regulators, nine potential genes were identified based on sequence similarity. Five of these CBF -like genes were found in a copy number variant (CNV) cluster of six genes on chromosome 14. Expression analysis showed that two homeologous genes ( ERF41 and ERF180 ) increased in abundance with cold and ethylene, while the cluster of CNV CBF -like genes had lost the ability to respond to cold and increased only with ethylene, suggesting an evolutionary progression of function of these genes.

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“…The regulation of fruit ripening by low temperature is not unique to citrus fruit. Previous studies have also demonstrated a role for low temperature, either independently or in concert with ethylene, in the regulation of fruit ripening in multiple fruit species such as kiwifruit (Mworia et al ., 2012; Asiche et al ., 2017; Asiche et al ., 2018; Mitalo et al, 2018a; Mitalo et al ., 2019a; Mitalo et al ., 2019b), pears (El-Sharkawy et al, 2003; Mitalo et al ., 2019c) and apples (Tacken et al ., 2010). From an ecological perspective, the primary purpose of fruit ripening is to make fruit attractive to seed-dispersing organisms.…”

Section: Discussionmentioning

confidence: 99%

“…However, promotion of fruit ripening by low temperature has been described in various fruit species such as kiwifruit (Kim et al ., 1999; Mworia et al ., 2012; Asiche et al ., 2017; Mitalo et al ., 2018a), European pears (El-Sharkawy et al ., 2003; Nham et al ., 2017), and apples (Tacken et al ., 2010). Recently, transcriptome studies have suggested that low temperature-specific genes might have regulatory roles during fruit ripening in kiwifruit (Asiche et al ., 2018; Mitalo et al ., 2018b; Mitalo et al ., 2019a; Mitalo et al ., 2019b) and European pears (Mitalo et al ., 2019c).…”

Section: Introductionmentioning

confidence: 99%

Low temperature transcriptionally modulates natural peel degreening in lemon (Citrus limon L.) fruit independently of endogenous ethylene

Mitalo

Otsuki

Okada

et al. 2019

Preprint

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AbstractPeel degreening is an important aspect of fruit ripening in many citrus fruit, and earlier studies have shown that it can be advanced either by ethylene treatment or during low temperature storage. However, the important regulators and pathways involved in natural peel degreening remain largely unknown. To understand how natural peel degreening is regulated in lemon (Citrus limon L.) fruit, flavedo transcriptome and physiochemical changes in response to either ethylene treatment or low temperature were studied. Ethylene treatment induced rapid peel degreening which was strongly inhibited by the ethylene antagonist, 1-methylcyclopropene (1-MCP). Compared with 25°C, moderately low temperatures (5°C, 10°C, 15°C and 20°C) also triggered peel degreening. Surprisingly, repeated 1-MCP treatments failed to inhibit the peel degreening induced by low temperature. Transcriptome analysis revealed that low temperature and ethylene independently regulated genes associated with chlorophyll degradation, carotenoid metabolism, photosystem proteins, phytohormone biosynthesis and signalling, and transcription factors. On-tree peel degreening occurred along with environmental temperature drops, and it coincided with the differential expression of low temperature-regulated genes. In contrast, genes that were uniquely regulated by ethylene showed no significant expression changes during on-tree peel degreening. Based on these findings, we hypothesize that low temperature plays a prominent role in regulating natural peel degreening independently of ethylene in citrus fruit.HighlightCitrus peel degreening is promoted by low temperature via modulation of multiple genes associated with chlorophyll degradation, carotenoid biosynthesis, photosystem disassembly, phytohormones and transcription factors without involving ethylene signalling.

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Comparative analysis of fruit ripening and associated genes in two kiwifruit cultivars (‘Sanuki Gold’ and ‘Hayward’) at various storage temperatures

Cited by 19 publications

References 43 publications

Low temperature modulates natural peel degreening in lemon fruit independently of endogenous ethylene

Low temperature modulates natural peel degreening in lemon fruit independently of endogenous ethylene

Copy number variants in kiwifruit ETHYLENE RESPONSE FACTOR/APETALA2 (ERF/AP2)-like genes show divergence in fruit ripening associated cold and ethylene responses in C-REPEAT/DRE BINDING FACTOR-like genes

Low temperature transcriptionally modulates natural peel degreening in lemon (Citrus limon L.) fruit independently of endogenous ethylene

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