Comparative Analysis of Far East Sikhotinsky Rhododendron (Rh. sichotense) and East Siberian Rhododendron (Rh. adamsii) Using Supercritical CO2-Extraction and HPLC-ESI-MS/MS Spectrometry

Razgonova, Mayya P.; Zakharenko, Alexander M.; Erċışlı, Sezai; Grudev, Vasily; Golokhvast, Kirill S.

doi:10.3390/molecules25173774

Cited by 21 publications

(9 citation statements)

References 30 publications

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“…A similar metabolite, myricetin, was previously detected in Rhododendron species, i.e., R. adamsii , R. sichotense , R. ambiguum , and R. luteum [ 8 , 14 , 21 ]. Its high concentration of 1.377 ± 0.012 mg/g DE) was similar to the amount determined in the leaves of R. adamsii [ 21 ]. Quercetin was another aglycone present in a substantial amount in RL-M (1.609 ± 0.011 mg/g DE).…”

Section: Resultssupporting

confidence: 56%

LC-ESI-MS/MS Characterization of Concentrated Polyphenolic Fractions from Rhododendron luteum and Their Anti-Inflammatory and Antioxidant Activities

2022

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The high biological potential of polyphenols encourages the search for new natural sources of and biomedical applications for these compounds. Rhododendron luteum Sweet was previously reported to contain pharmaceutically active polyphenols. The present research investigates the polyphenolic fractions in R. luteum leaves, including a determination of the free and bound phenolic acid and flavonoid contents and their anti-inflammatory and antioxidant activities. LC-ESI-MS/MS (liquid chromatography/electrospray ionization triple quadrupole mass spectrometry) analysis revealed a great abundance of free (e.g., 5-O-caffeoylquinic acid, ferulic acid, protocatechuic acid, catechin, and dihydromyricetin) and bound (e.g., caffeic acid, p-coumaric, protocatechuic acid, myricetin, quercetin) phenolics. The R. luteum samples exhibited high anti-inflammatory potential in lipoxygenase (IC50: 0.33 ± 0.01–2.96 ± 0.06 mg dry extract (DE)/mL) and hyaluronidase (IC50: 78.76 ± 2.09 – 429.07 ± 31.08 µg DE/mL) inhibition capacity assays. Some samples also had the ability to inhibit cyclooxygenase 1 (IC50: 311.8 ± 10.95 µg DE/mL) and cyclooxygenase 2 (IC50: 53.40 ± 5.07; 608.09 ± 14.78 µg DE/mL). All fractions showed excellent antioxidant activity in the Oxygen Radical Absorbance Capacity (ORAC) assay (5.76–221.81 g Trolox/g DE), ABTS•+ radical scavenging ability (0.62 ± 0.03 – 5.09 ± 0.23 g Trolox/g DE), and moderate ion (Fe2+) chelating power. This paper expands our knowledge of the phytochemistry and pharmacological activity of R. luteum polyphenols. It reveals, for the first time, the presence of dihydromyricetin, afzelin, and laricitrin in the plant material. It indicates biologically active polyphenolic fractions that should be further investigated or which could be efficiently used in pharmaceutical, cosmetic, or nutraceutical applications.

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Section: Resultssupporting

confidence: 56%

LC-ESI-MS/MS Characterization of Concentrated Polyphenolic Fractions from Rhododendron luteum and Their Anti-Inflammatory and Antioxidant Activities

2022

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“…High-performance liquid chromatography (HPLC) in combination with tandem mass spectrometry was used to identify target analytes in the extracts. Previously, the authors carried out metabolomic studies of Far Eastern plant species, such as Schizandra chinensis, Rhodiola rosea, Rhododendron adamsii, and Panax ginseng [14,15].…”

Section: Introductionmentioning

confidence: 99%

LC-MS/MS Screening of Phenolic Compounds in Wild and Cultivated Grapes Vitis amurensis Rupr.

et al. 2021

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This work represents a comparative metabolomic study of extracts of wild grapes obtained from six different places in the Primorsky and Khabarovsk territories (Far East Russia) and extracts of grapes obtained from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources (St. Petersburg). The metabolome analysis was performed by liquid chromatography in combination with ion trap mass spectrometry. The results showed the presence of 118 compounds in ethanolic extracts of V. amurensis grapes. In addition, several metabolites were newly annotated in V. amurensis. The highest diversity of phenolic compounds was identified in the samples of the V. amurensis grape collected in the vicinity of Vyazemsky (Khabarovsk Territory) and the floodplain of the Arsenyevka River (Primorsky Territory), compared to the other wild samples and cultural grapes obtained in the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources.

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“…However, when extra 5% chloroform was added to the extraction system as a co-solvent, it significantly increased the yields of the total fatty acid fraction. The choice of the solvent and the extraction range parameters was based on the results of previous works where starfish material was treated with a plant matrix [4,5]. Despite the fact that the main target components are soluble in liquid CO 2 only above 200 bar, it is possible to separate significant amounts of the lipid fraction [6,7].…”

Section: Resultsmentioning

confidence: 99%

Supercritical Extraction Technology of Obtaining Polyunsaturated Acids from Starfish (Lysastrosoma anthosticta Fisher, 1922)

Zakharenko

Kirichenko

Vakhniuk

et al. 2022

Food Processing: Techniques and Technology

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Introduction. Starfish (Asteroidea) are marine echinoderms with more than 160 species. Starfish are a valuable source of protein and fats. The present research featured the chemical composition of starfish, which can be used as a commercial source of lipids. Study objects and methods. The study defined the optimal parameters for extracting the lipid fraction of Lysastrosoma anthosticta with supercritical carbon dioxide, as well as the qualitative composition of the obtained extracts. Results and discussion. The yield of fatty acids obtained with supercritical carbon dioxide co-solvent was 1.8 times higher than that obtained with standard extraction according to the Folch method. The content of impurities was lower than in the samples with chloroform-methanol system. The polyunsaturated fatty acids isolated from L. anthosticta mainly belonged to ω-3 (18.0%), ω-6 (11.7%), ω-7 (21.2%), ω-9 (10.1%), and ω-11 (6.5%). The rest was saturated fatty acids, mainly palmitic (14%) and myristic (6%). The qualitative composition of the lipid fraction did not depend significantly from the isolation method. However, the supercritical extraction increased the product yield, extraction rate, and the quality of the extraction residue. Supercritical carbon dioxide left a dry residue, which had no typical smell and was brittle enough for grinding. Such residue can presumably be used to produce protein concentrate. Conclusion. Supercritical extraction with chloroform can be recommended to isolate fatty acids from marine organisms at 60°C and 400 bar.

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Comparative Analysis of Far East Sikhotinsky Rhododendron (Rh. sichotense) and East Siberian Rhododendron (Rh. adamsii) Using Supercritical CO2-Extraction and HPLC-ESI-MS/MS Spectrometry

Cited by 21 publications

References 30 publications

LC-ESI-MS/MS Characterization of Concentrated Polyphenolic Fractions from Rhododendron luteum and Their Anti-Inflammatory and Antioxidant Activities

LC-ESI-MS/MS Characterization of Concentrated Polyphenolic Fractions from Rhododendron luteum and Their Anti-Inflammatory and Antioxidant Activities

LC-MS/MS Screening of Phenolic Compounds in Wild and Cultivated Grapes Vitis amurensis Rupr.

Supercritical Extraction Technology of Obtaining Polyunsaturated Acids from Starfish (Lysastrosoma anthosticta Fisher, 1922)

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