Comparative analysis of epicuticular lipid profiles of sympatric and allopatric field populations of Anopheles gambiae s.s. molecular forms and An. arabiensis from Burkina Faso (West Africa)

Caputo, Beniamino; Dani, Francesca Romana; Horne, G.L.; Sagnon, N’Falé; Diabaté, Abdoulaye; Turillazzi, Stefano; Cóluzzi, M.; Costantini, Carlo; Priestman, A.; Petrarca, Vincenzo; Torre, Alessandra della

doi:10.1016/j.ibmb.2007.01.002

Cited by 39 publications

(22 citation statements)

References 44 publications

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“…As mentioned above, a caveat underlying the MALDI-TOF MS is that the full complement of proteins and peptides cannot be detected. Similarly, it might also miss out on detecting differences between epicuticular lipid profiles that have been described between A. gambiae M and S molecular forms as well as A. arabiensis as they are below the detection range [51].…”

Section: Resultsmentioning

confidence: 99%

Identification of Cryptic Anopheles Mosquito Species by Molecular Protein Profiling

Pflüger²,

et al. 2013

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Vector control is the mainstay of malaria control programmes. Successful vector control profoundly relies on accurate information on the target mosquito populations in order to choose the most appropriate intervention for a given mosquito species and to monitor its impact. An impediment to identify mosquito species is the existence of morphologically identical sibling species that play different roles in the transmission of pathogens and parasites. Currently PCR diagnostics are used to distinguish between sibling species. PCR based methods are, however, expensive, time-consuming and their development requires a priori DNA sequence information. Here, we evaluated an inexpensive molecular proteomics approach for Anopheles species: matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS is a well developed protein profiling tool for the identification of microorganisms but so far has received little attention as a diagnostic tool in entomology. We measured MS spectra from specimens of 32 laboratory colonies and 2 field populations representing 12 Anopheles species including the A. gambiae species complex. An important step in the study was the advancement and implementation of a bioinformatics approach improving the resolution over previously applied cluster analysis. Borrowing tools for linear discriminant analysis from genomics, MALDI-TOF MS accurately identified taxonomically closely related mosquito species, including the separation between the M and S molecular forms of A. gambiae sensu stricto. The approach also classifies specimens from different laboratory colonies; hence proving also very promising for its use in colony authentication as part of quality assurance in laboratory studies. While being exceptionally accurate and robust, MALDI-TOF MS has several advantages over other typing methods, including simple sample preparation and short processing time. As the method does not require DNA sequence information, data can also be reviewed at any later stage for diagnostic or functional patterns without the need for re-designing and re-processing biological material.

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Section: Resultsmentioning

confidence: 99%

Identification of Cryptic Anopheles Mosquito Species by Molecular Protein Profiling

Pflüger²,

et al. 2013

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“…Weak but inconclusive evidence for differences in composition of cuticular hydrocarbons between the molecular forms were detected (Caputo et al, 2007). Additional evidence against olfactory cues involvement in mate recognitions was obtained from studies on indoor mating in Mali.…”

Section: Phenotypic Divergence Between the Molecular Formsmentioning

confidence: 99%

The molecular forms of Anopheles gambiae: A phenotypic perspective

Lehmann

Diabaté

2008

Infection, Genetics and Evolution

152

189

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The African malaria mosquito Anopheles gambiae is undergoing speciation, being split into the M and S molecular forms. Speciation is the main process promoting biological diversity, thus, new vector species might complicate disease transmission. Genetic differentiation between the molecular forms has been extensively studied, but phenotypic differences between them, the evolutionary forces that generated divergence, and the mechanisms that maintain their genetic isolation have only recently been addressed. Here, we review recent studies suggesting that selection mediated by larval predation and competition promoted divergence between temporary and permanent freshwater habitats. These differences explain the sharp discontinuity in distribution of the molecular forms between rice fields and surrounding savanna, but they can also explain the concurrent cline between humid and arid environments due to the dependence on permanent habitats in the latter. Although less pronounced, differences in adult body size, reproductive output, and longevity also suggest that the molecular forms have adapted to distinct niches. Reproductive isolation between the molecular forms is achieved by spatial swarm segregation, although within-swarm mate recognition appears to play a role in certain locations. The implications of these results to disease transmission and control are discussed and many of the gaps in our understanding are highlighted.

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“…CHC have also been employed to differentiate hematophagous disease vector species of the genera Glossina, Simulium, and Anopheles (Carlson and Service 1979, Phillips et al 1985, Milligan et al 1986, Carlson et al 1993. In Anopheles sp., some studies have shown that variations in CHC relative abundance were correlated to the geographic origin of the specimens (Caputo et al 2007). Distinctive CHC patterns for a variety of triatomine species are already known (Juárez and Brenner 1986;Juárez et al 2000Juárez et al , 2002.…”

mentioning

confidence: 99%

Cuticular Hydrocarbons of Triatoma dimidiata (Hemiptera: Reduviidae): Intraspecific Variation and Chemotaxonomy

Calderón-Fernández

Girotti

Juárez³

2011

jnl. med. entom.

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Triatoma dimidiata Latreille is a major vector of Chagas disease with an extensive geographic distribution from Central Mexico, through Central America, to northern South America. As a result of its variability in phenetic and genetic characters, disagreement concerning its taxonomic status has been raised. In this study, the cuticular hydrocarbon pattern of T. dimidiata populations from Mexico, Belize, Guatemala, Honduras, Costa Rica, and Colombia was analyzed by capillary gas chromatography coupled to mass spectrometry; linear discriminant analysis was used to help elucidate population structure. Vector populations segregated into five distinct groups; specimens from Yucatan Peninsula, together with those from Central Mexico, Central America, and Colombia corresponded to different T. dimidiata subspecies, a putative different species comprising insects from Belize, together with an isolated population collected at bat caves in Guatemala. The analysis revalidates the earlier division of T dimidiata into three subspecies, T. d. maculipennis, T. d. dimidiata, and T. d. capitata; and an additional subspecies and a distinct species are proposed.

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Comparative analysis of epicuticular lipid profiles of sympatric and allopatric field populations of Anopheles gambiae s.s. molecular forms and An. arabiensis from Burkina Faso (West Africa)

Cited by 39 publications

References 44 publications

Identification of Cryptic Anopheles Mosquito Species by Molecular Protein Profiling

Identification of Cryptic Anopheles Mosquito Species by Molecular Protein Profiling

The molecular forms of Anopheles gambiae: A phenotypic perspective

Cuticular Hydrocarbons of Triatoma dimidiata (Hemiptera: Reduviidae): Intraspecific Variation and Chemotaxonomy

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