Comparative analysis of cutaneous bacterial communities of farmed Rana dybowskii after gentamycin bath

Abstract

Introduction Pathogenic bacteria limit the success of Rana dybowskii breeding. Gentamicin is used to treat R. dybowskii disease. To understand the effects of gentamicin on the composition and structure of the cutaneous bacterial community of R. dybowskii, three groups (control, gentamicin and recovery) were established in this study. Materials & Methods The V3–V4 hypervariable region of the 16S rRNA gene was analyzed in samples by high-throughput sequencing. Alpha diversity and beta diversity were evaluated to compare the cutaneous bacterial community diversity. Results A total of 1,159,668 valid sequences and 3,132 operational taxonomic units (OTUs) were obtained from these three experimental groups. The number of OTUs obtained in the control group, gentamicin group and recovery group were 2,194, 2,288, and 2,047, respectively, and the number of shared OTUs was 1,313. The alpha diversity of the cutaneous bacterial community was not significantly affected by gentamicin, while beta diversity was significantly affected. Discussion & Conclusions The effect of a gentamicin bath on relative species abundance was greater than the effect on the species composition. The changes in Proteobacteria, Acinetobacter, and Chryseobacterium were significant, and reductions were observed after the recovery period. Six potentially pathogenic genera were detected, including Aeromonas, Citrobacter, Chryseobacterium, Pseudomonas, Staphylococcus, and Streptococcus. Among them, Aeromonas and Chryseobacterium were significantly inhibited by the gentamicin bath. The results of this study provide a theoretical basis for the application of gentamicin in R. dybowskii breeding.

Changes in the Gut Microbiota Diversity of Brown Frogs (Rana Dybowskii) After Local Antimicrobial Therapy

“…Providing medicine in a bath is also a good method for animals. In this study, gentamicin was used at a dose of 20 mg/L, which is close to the dose used in sh seedlings in aquaculture [12]. The drug bath method is non-invasive with less stress to animals than other methods, possibly reducing animal pain and damage to the skin [11].…”

“…However, transdermal absorption also involves the passive diffusion of a chemical through the epidermis into the capillaries of the dermis [11]. The gills and skin of amphibians are highly permeable, resulting in their introduction as a remarkable indicator for assessing the harmful effects of pollution [12]. In research, antibiotics are frequently employed to examine the role of the microbiota.…”

“…Rana dybowskii (brown frog) is a major aquaculture species with medical and nutritional values in China [18]. The current stagnation of the frog culture industry is mainly caused by diseases, primarily bacterial diseases [12]. If a disease is found during the breeding process of Rana dybowskii, it is di cult to carry out effective treatment [12].…”

“…The current stagnation of the frog culture industry is mainly caused by diseases, primarily bacterial diseases [12]. If a disease is found during the breeding process of Rana dybowskii, it is di cult to carry out effective treatment [12]. Mainly, frogs are ingested quickly after the onset of disease, and drugs are di cult to administer orally; furthermore, due to the number of farmed frogs, small individuals and susceptibility to stress, the intramuscular injection and intravenous injection methods are di cult to achieve [12].…”

“…If a disease is found during the breeding process of Rana dybowskii, it is di cult to carry out effective treatment [12]. Mainly, frogs are ingested quickly after the onset of disease, and drugs are di cult to administer orally; furthermore, due to the number of farmed frogs, small individuals and susceptibility to stress, the intramuscular injection and intravenous injection methods are di cult to achieve [12]. Therefore, frog disease prevention and appropriate methods of administration are particularly important.…”