Comparative analysis of clastogen-induced chromosome aberrations observed with light microscopy and by means of atomic force microscopy

Koleva, Vanya; Dragoeva, Asya; Andreeva, A.; Burova, M.; Georgiev, S.; Enchev, D. D.

doi:10.1016/j.mrgentox.2012.12.014

Cited by 6 publications

(2 citation statements)

References 54 publications

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“…Also, AFM has been used to study the effect of specific radiations on biological structure, 29 such as chromosome aberrations 30,31 caused by ionizing radiation and clastogen. 32 However, the micro−nano-structural aberrations of chromosomes caused by UVB irradiation have not been reported. In this work, HaCaT cells were exposed to UVB irradiation, and irradiation-induced chromosomal structural aberration was observed by AFM.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Fan et al studied the ultrastructure of G 1 -phase chromosomes by AFM and obtained the 30 nm chromatin fiber structure. Also, AFM has been used to study the effect of specific radiations on biological structure, such as chromosome aberrations , caused by ionizing radiation and clastogen . However, the micro–nano-structural aberrations of chromosomes caused by UVB irradiation have not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Use of Atomic Force Microscopy in UVB-Induced Chromosome Damage Provides Important Bioinformation for Cell Damage Assessment

Wang,

Dong,

Yang

et al. 2023

Langmuir

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The chromosomal structure derived from UVB-stimulated HaCaT cells was detected by atomic force microscopy (AFM) to evaluate the effect of UVB irradiation. The results showed that the higher the UVB irradiation dose, the more the cells that had chromosome aberration. At the same time, different representative types of chromosome structural aberrations were investigated. We also revealed damage to both DNA and cells under the corresponding irradiation doses. It was found that the degree of DNA damage was directly proportional to the irradiation dose. The mechanical properties of cells were also changed after UVB irradiation, suggesting that cells experienced a series of chain reactions from inside to outside after irradiation. The highresolution imaging of chromosome structures by AFM after UVB irradiation enables us to relate the damage between chromosomes, DNA, and cells caused by UVB irradiation and provides specific information on genetic effects.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of Atomic Force Microscopy in UVB-Induced Chromosome Damage Provides Important Bioinformation for Cell Damage Assessment

Wang,

Dong,

Yang

et al. 2023

Langmuir

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Atomic force microscopy imaging of the G-banding process of chromosomes

Wang

Dong

et al. 2020

Appl Nanosci

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The chromosome is an important genetic material carrier in living individuals and the spatial conformation (mainly referring to the chromosomal structure, quantity, centromere position and other morphological information) may be abnormal or mutated. Thus, it may generate a high possibility to cause diseases. Generally, the karyotype of chromosome G-bands is detected and analyzed using an optical microscope. However, it is difficult to detect the G-band structures for traditional optical microscopes on the nanometer scale. Herein, we have studied the detection method of chromosome G-band samples by atomic force microscopy (AFM) imaging.The structures of chromosome G-banding are studied with different trypsin treatment durations. The experiment result shows that the treatment duration of 20 s is the best time to form G-band structures. The AFM images show the structures of chromosome G-bands which cannot be observed under an optical microscope. This work provides a new way for the detection and diagnosis of chromosome diseases on the nanometer scale.

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Increased levels of chromosomal aberrations and DNA damage in a group of workers exposed to formaldehyde

et al. 2015

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Formaldehyde (FA) is a commonly used chemical in anatomy and pathology laboratories as a tissue preservative and fixative. Because of its sensitising properties, irritating effects and cancer implication, FA accounts probably for the most important chemical-exposure hazard concerning this professional group. Evidence for genotoxic effects and carcinogenic properties in humans is insufficient and conflicting, particularly in regard to the ability of inhaled FA to induce toxicity on other cells besides first contact tissues, such as buccal and nasal cells. To evaluate the effects of exposure to FA in human peripheral blood lymphocytes, a group of 84 anatomy pathology laboratory workers exposed occupationally to FA and 87 control subjects were tested for chromosomal aberrations (CAs) and DNA damage (comet assay). The level of exposure to FA in the workplace air was evaluated. The association between genotoxicity biomarkers and polymorphic genes of xenobiotic-metabolising and DNA repair enzymes were also assessed. The estimated mean level of FA exposure was 0.38±0.03 ppm. All cytogenetic endpoints assessed by CAs test and comet assay % tail DNA (%TDNA) were significantly higher in FA-exposed workers compared with controls. Regarding the effect of susceptibility biomarkers, results suggest that polymorphisms in CYP2E1 and GSTP1 metabolic genes, as well as, XRCC1 and PARP1 polymorphic genes involved in DNA repair pathways are associated with higher genetic damage in FA-exposed subjects. Data obtained in this study show a potential health risk situation of anatomy pathology laboratory workers exposed to FA (0.38 ppm). Implementation of security and hygiene measures may be crucial to decrease risk. The obtained information may also provide new important data to be used by health care programs and by governmental agencies responsible for occupational health and safety.

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Comparative analysis of clastogen-induced chromosome aberrations observed with light microscopy and by means of atomic force microscopy

Cited by 6 publications

References 54 publications

Use of Atomic Force Microscopy in UVB-Induced Chromosome Damage Provides Important Bioinformation for Cell Damage Assessment

Use of Atomic Force Microscopy in UVB-Induced Chromosome Damage Provides Important Bioinformation for Cell Damage Assessment

Atomic force microscopy imaging of the G-banding process of chromosomes

Increased levels of chromosomal aberrations and DNA damage in a group of workers exposed to formaldehyde

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