Comparative analysis of cell cycle events in zygotic and somatic embryos of Cyclamen persicum indicates strong resemblance of somatic embryos to recalcitrant seeds

Schmidt, Th.; Ewald, A.; Seyring, M.; Hohe, Annette

doi:10.1007/s00299-006-0130-9

Cited by 15 publications

(20 citation statements)

References 18 publications

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“…Over the past 20 years, various studies on somatic embryo development have been conducted, but most were limited to physiological description (Ruffoni et al 2000) or on improvement of culture methods (Kiviharju et al 1992;Kreuger et al 1995;Schwenkel and Winkelmann 1998). As Cyclamen embryos are relatively large in size, they offer advantages over those of D. carota or Arabidopsis thaliana for studying the mechanism of somatic embryogenic development analyses; C. persicum is a well-described system for somatic embryogenesis study in dicotylendons (Schmidt et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Somatic Embryogenesis in Cyclamen persicum Mill

Bian

Zheng

et al. 2009

Plant Mol Biol Rep

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Cyclamen persicum Mill. is a widely grown ornamental species that is clonally propagated by somatic embryogenesis. To better understand the biology of somatic embryo development in C. persicum, detailed proteomic (two-dimensional gel electrophoresis) and mass spectrometric analyses of somatic embryos at globular, torpedo, and germinating stages of development, along with nonembryogenic callus and zygotic embryos, were conducted. Of~460 proteins resolved in two-dimensional gels, 35 proteins were differentially expressed and could be reproducibly displayed across an isoelectric focusing range of 5 to 8. Among those proteins, five were constitutively expressed, 13 were upregulated, nine were downregulated, and eight were deemed as novel proteins during the torpedo stage. A total of 35 protein spots were analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and only four proteins were identified and these were available in public protein databases. The remaining protein spots were subsequently analyzed by MALDI-TOF-TOF-MS, and six proteins were then identified. These findings suggested that specific proteins are involved in the regulation of somatic embryogenesis.

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Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Somatic Embryogenesis in Cyclamen persicum Mill

Bian

Zheng

et al. 2009

Plant Mol Biol Rep

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“…For example, You et al (2011) noted that some C. persicum cultivars were recalcitrant to somatic embryogenesis. C. persicum showed very low levels of somatic embryogenesis when mature tissues or organs were used as explants Schmidt et al 2006). In addition, there was a considerable decrease in the embryogenic competence of C. persicum callus as subcultures increased Winkelmann et al 2004).…”

Section: Callus Induction From Different Explant Types and Pgrs Of Enmentioning

confidence: 98%

Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey

İzgü

Sevindik

Çürük

et al. 2016

Plant Cell Tiss Organ Cult

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In this study, embryo-like structures (ELSs) were induced in four endemic Turkish Cyclamen species (C. cilicium Boiss. et Heldr., C. parviflorum Pobed., C. mirabile Hildebr. and C. pseudibericum Hildebr.) in the presence of 13 combinations of two plant growth regulators (PGRs) (2,4-dichlorophenoxyacetic acid and 6-(c,cdimethylallylamino)purine) and four explant types (ovules, ovaries, leaves and petioles). The ratio of callus induction, different stages of ELS formation and the conversion of ELSs to plantlets were quantified. The most effective explant types for callus induction were leaves (56 % for C. cilicium and 59 % for C. parviflorum) and petioles (80 % for C. mirabile and 100 % for C. pseudibericum). Callus growth from the leaves and petioles of C. cilicium was 30 days earlier than that of C. mirabile and C. pseudibericum. In contrast, most callus formed from the petioles of C. pseudibericum (100 %) in medium with 2.5 mg l -1 2,4-D and 1 mg l -1 2iP. The highest number of ELSs was obtained succesfully from petioles (2.5 mg l -1 2,4-D and 1 mg l -1 2iP) and ovaries (2.5 mg l -1 2,4-D and 0.5 mg l -1 2iP) of C. pseudibericum, in 39 % and as 32 % of explants, respectively. The percentage conversion of ELSs to plantlets was 38, 31, 16 and 15 % for C. mirabile, C. cilicium, C. pseudibericum and C. parviflorum, respectively. The plantlets were successfully acclimatized in the greenhouse with 54, 70, 63 and 25 % of C. cilicium, C. mirabile, C. pseudibericum and C. parviflorum plantlets, respectively surviving after transfer to ex vitro conditions. This paper describes a unique, reliable and consistent protocol for the induction of ELSs from four endangered endemic Turkish Cyclamen species, opening up the possibility of preserving these valuable genetic resources in vitro and also other applied biotechnologies that rely on a stable embryogenic or callus-based protocol.

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“…Starting from embryogenic callus, the removal of auxin, i.e. the culture on plant growth regulator (PGR) free medium, leads to the realisation of embryogenesis undergoing the typical stages as globular and finally torpedo-shaped embryos within 4 weeks (Schmidt et al 2006). After germination of these embryos healthy and genetically identical plants can be transferred to the greenhouse.…”

Section: Introductionmentioning

confidence: 99%

“…Somatic embryos of C. persicum have been shown to lack maturation-related characteristics like the accumulation of storage compounds (Winkelmann et al 2006;Rode et al 2011), desiccation (Winkelmann et al 2004) and a quiescence period (Schmidt et al 2006). This may correlate with inhomogeneous development and premature germination.…”

Section: Introductionmentioning

confidence: 99%

From callus to embryo: a proteomic view on the development and maturation of somatic embryos in Cyclamen persicum

Rode

Lindhorst

Braun

et al. 2011

Planta

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In this study, the proteome structures following the pathway in somatic embryogenesis of Cyclamen persicum were analysed via high-resolution 2D-SDS-PAGE with two objectives: (1) to identify the significant physiological processes during somatic embryogenesis in Cyclamen and (2) to improve the maturation of somatic embryos. Therefore, the effects of maturation-promoting plant growth regulator abscisic acid (ABA) and high sucrose levels on torpedo-shaped embryos were investigated. In total, 108 proteins of differential abundance were identified using a combination of tandem mass spectrometry and a digital proteome reference map. In callus, enzymes related to energy supply were especially distinct, most likely due to energy demand caused by fast growth and cell division. The switch from callus to globular embryo as well as from globular to torpedo-shaped embryo was associated with controlled proteolysis via the ubiquitin-26S proteasome pathway. Storage compound accumulation was first detected 21 days after transfer to plant growth regulator (PGR)-free medium in early torpedo-shaped embryos. Increase in abundance of auxin-amidohydrolase during embryogenesis suggests a possible increase in auxin release in the late embryo stages of Cyclamen. A development-specific isoelectric point switch of catalases has been reported for the first time for somatic embryogenesis. Several proteins were identified to represent markers for the different developmental stages analysed. High sucrose levels and ABA treatment promoted the accumulation of storage compounds in torpedo-shaped embryos. Additionally, proteins of the primary metabolic pathways were decreased in the proteomes of ABA-treated embryos. Thus, ABA and high sucrose concentration in the culture medium improved maturation and consequently the quality of somatic embryos in C. persicum.

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Comparative analysis of cell cycle events in zygotic and somatic embryos of Cyclamen persicum indicates strong resemblance of somatic embryos to recalcitrant seeds

Cited by 15 publications

References 18 publications

Proteomic Analysis of Somatic Embryogenesis in Cyclamen persicum Mill

Proteomic Analysis of Somatic Embryogenesis in Cyclamen persicum Mill

Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey

From callus to embryo: a proteomic view on the development and maturation of somatic embryos in Cyclamen persicum

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