Comparative analysis of biological effects of molybdenum(IV) sulfide in the form of nano- and microparticles on human hepatoma HepG2 cells grown in 2D and 3D models

Sobańska, Zuzanna; Domeradzka-Gajda, Katarzyna; Szparaga, Mateusz; Grobelny, Jarosław; Tomaszewska, Emilia; Ranoszek-Soliwoda, Katarzyna; Celichowski, Grzegorz; Zapór, Lidia; Kowalczyk, K.; Stępnik, Maciej

doi:10.1016/j.tiv.2020.104931

Cited by 8 publications

(11 citation statements)

References 34 publications

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“…For a given bioactive food compound, there are many variables affected by its preparation and processing methods that influence its bioavailability, efficacy, and toxicities; these variables include but are not limited to particle size, purity, and crystallinity, cross-linking, and entanglement. For a given compound, delivery methods (i.e., nanoparticles) to improve the bioavailability of the compound are another emerging research area 67 , 68 . Collectively, these factors generate a considerable bottleneck.…”

Section: Discussionmentioning

confidence: 99%

Advances in 3D peptide hydrogel models in cancer research

Wang

et al. 2021

npj Sci Food

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In vitro cell culture models on monolayer surfaces (2D) have been widely adapted for identification of chemopreventive food compounds and food safety evaluation. However, the low correlation between 2D models and in vivo animal models has always been a concern; this gap is mainly caused by the lack of a three-dimensional (3D) extracellular microenvironment. In 2D models, cell behaviors and functionalities are altered, resulting in varied responses to external conditions (i.e., antioxidants) and hence leading to low predictability. Peptide hydrogel 3D scaffolding technologies, such as PGmatrix for cell culture, have been recently reported to grow organoid-like spheroids physiologically mimicking the 3D microenvironment that can be used as an in vitro 3D model for investigating cell activities, which is anticipated to improve the prediction rate. Thus, this review focuses on advances in 3D peptide hydrogels aiming to introduce 3D cell culture tools as in vitro 3D models for cancer-related research regarding food safety and nutraceuticals.

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Section: Discussionmentioning

confidence: 99%

Advances in 3D peptide hydrogel models in cancer research

Wang

et al. 2021

npj Sci Food

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“…The well-differentiated hepatoma cell line HepG2, which has a wide set of properties inherent to human hepatocytes in vivo, has been actively used for about 40 years in various studies exploiting organ-specific features of the line [ 10 , 11 , 34 , 35 , 36 , 37 ]. Morphology of HepG2 cells was shown to keep main features of the hepatocytes: tight junctions separate apical and basolateral plasmalemma providing formation of bile capillaries and blood-biliary barrier; ultrastructural observations were supported by immunohistochemical and biochemical studies [ 38 , 39 ].…”

Section: Resultsmentioning

confidence: 99%

“…Previously published studies showed changes in the shape and compactization of HepG2 spheroids during their development; early, middle and late stages of spheroid growth were identified, the duration of which varied in different works and mainly depended on the dose of cell seeding. Common recommendation was to work with HepG2 spheroids at middle stage (beginning from days 4–5 after cell seeding) which is characterized by active function of spheroid cells and absence of cell destruction [ 10 , 14 , 45 ]. Amount of published studies using HEK293 spheroids is incomparably less than studies with HepG2 spheroids; however, authors noted a very fast compactization of HEK293 cells on a non-adhesive surface and regular spherical shape of the spheroids [ 46 , 47 ].…”

Section: Resultsmentioning

confidence: 99%

“…Interest to penetration of a drug into spheroid tissue is related with known block of the diffusion in tissue of solid tumors in vivo influencing drug effect [ 63 ]. Published studies show that the penetration of various NPs into HepG2 and other spheroids is limited to a depth of 20–50 µm, despite a fairly long incubation (for 24–72 h) [ 10 , 15 , 64 ]. The same values of penetration into HepG2 spheroids were reported for sorafenib [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

“…Advantages of using gold nanoparticles (AuNPs) and their various modifications in the treatment and diagnosis of diseases are being actively studied; a number of comprehensive detailed reviews is devoted to this issue [ 1 , 2 , 3 , 4 , 5 , 6 ]. Similar to other NPs, AuNPs are studied in cell cultures and in laboratory animals; and in last decade a new experimental model has been developed: multicellular spheroids or micro-tissues (cell cultures in 3D-form); the advantages of spheroids are described in details [ 7 , 8 , 9 , 10 ]. Spheroids that mimic the structure and functions of various tissues have shown their suitability for studies of different problems in modern biomedicine, including the effects of drugs, drug damage to the liver, toxicity of chemical compounds, and human hepatocarcinoma (HepG2) spheroids are considered in such studies as a practically adequate replacement of primary hepatocytes [ 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Ultrastructural Features of Gold Nanoparticles Interaction with HepG2 and HEK293 Cells in Monolayer and Spheroids

et al. 2020

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Use of multicellular spheroids in studies of nanoparticles (NPs) has increased in the last decade, however details of NPs interaction with spheroids are poorly known. We synthesized AuNPs (12.0 ± 0.1 nm in diameter, transmission electron microscopy (TEM data) and covered them with bovine serum albumin (BSA) and polyethyleneimine (PEI). Values of hydrodynamic diameter were 17.4 ± 0.4; 35.9 ± 0.5 and ±125.9 ± 2.8 nm for AuNPs, AuBSA-NPs and AuPEI-NPs, and Z-potential (net charge) values were −33.6 ± 2.0; −35.7 ± 1.8 and 39.9 ± 1.3 mV, respectively. Spheroids of human hepatocarcinoma (HepG2) and human embryo kidney (HEK293) cells (Corning ® spheroid microplates CLS4515-5EA), and monolayers of these cell lines were incubated with all NPs for 15 min–4 h, and fixed in 4% paraformaldehyde solution. Samples were examined using transmission and scanning electron microscopy. HepG2 and HEK2893 spheroids showed tissue-specific features and contacted with culture medium by basal plasma membrane of the cells. HepG2 cells both in monolayer and spheroids did not uptake of the AuNPs, while AuBSA-NPs and AuPEI-NPs readily penetrated these cells. All studied NPs penetrated HEK293 cells in both monolayer and spheroids. Thus, two different cell cultures maintained a type of the interaction with NPs in monolayer and spheroid forms, which not depended on NPs Z-potential and size.

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Understanding Nanomaterial–Liver Interactions to Facilitate the Development of Safer Nanoapplications

Chen

Xia

2022

Advanced Materials

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vaccines, and drug carriers (Figure 1). The functional advantages of NMs are derived from their unique physical and chemical properties. [1,[4][5][6] Among all the NMs, metallic NMs including metal and metal oxides (MOx) nanoparticles (NPs), such as Ag and Au NPs, transition-metal oxides (TMOs, e.g., SiO 2 , Co 3 O 4 , and Mn 2 O 3 ), and rare-earth oxides REOs (e.g., Gd 2 O 3 , La 2 O 3 , and Y 2 O 3 ) NPs, are among the most produced NMs worldwide. Also, they are widely used in consumer products such as dietary supplements, fuel additives, cosmetics, bioimaging, and drug carriers, etc. due to their properties such as small size, high surface area, controlled particle shape as well as superior mechanical, electronic, optical, and magnetic properties. [7][8][9] For example, SiO 2 NPs have been applied in the theranostic fields, including bioimaging and targeted drug delivery. [7] In addition, the popularity of carbon nanomaterials (CNMs) including 0D fullerenes, 1D carbon nanotubes (CNTs), and 2D graphene-based nanoparticles (GBN) including graphene, graphene oxide (GO), and reduced graphene oxide (rGO) has been on the rise for their applications in battery, electronics, drug delivery, bio-sensing, bio-imaging, and tissue engineering due to their large surface area, diverse surface functional groups, excellent optical property, and efficient drug-loading capacity. [10][11][12][13][14][15][16] For example, an advanced NMsbased biosensing platform based on rGO has been developed to detect the coronavirus disease 2019 (COVID-19) antibodies within seconds. [17] Additionally, a form of nanostructured cellulose (nanocellulose), including cellulose nanocrystal (CNC) and cellulose nanofiber (CNF) has been increasingly considered for applications in papermaking, coatings, food, nanocomposite formulations and reinforcement, and biomedical fields (e.g., wound dressing) due to its biocompatibility, outstanding mechanical, chemical, and rheological properties. [18,19] Also 2D transition metal dichalcogenides (TMDs) (including MoS 2 and BN) with high surface area and free surface energy levels are increasingly being used for commercial applications in energy generation, sensors, catalysis, electronics, and biomedicine fields. [13,[20][21][22] Similarly, organic NPs (including lipid, liposome, polymer, micelle, dendrimer, and protein/peptide-based NPs) have been widely used in diagnosis, drug delivery, bioimaging, and cancer therapy because of their facile synthesis and chemical modification, self-assembly, biocompatibility, and biodegradability. [23][24][25] The global market value of NMs will be expected to reach US$ 125 billion marks by 2024. [26] The widespread Nanomaterials (NMs) are widely used in commercial and medical products, such as cosmetics, vaccines, and drug carriers. Exposure to NMs via various routes such as dermal, inhalation, and ingestion has been shown to gain access to the systemic circulation, resulting in the accumulation of NMs in the liver. The unique organ structures and blood flow features facilitate...

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Comparative analysis of biological effects of molybdenum(IV) sulfide in the form of nano- and microparticles on human hepatoma HepG2 cells grown in 2D and 3D models

Cited by 8 publications

References 34 publications

Advances in 3D peptide hydrogel models in cancer research

Advances in 3D peptide hydrogel models in cancer research

Ultrastructural Features of Gold Nanoparticles Interaction with HepG2 and HEK293 Cells in Monolayer and Spheroids

Understanding Nanomaterial–Liver Interactions to Facilitate the Development of Safer Nanoapplications

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