Comparative Analysis of Antioxidative Activity of Flavonoids Using HPLC–ED and Photometric Assays

Piszcz, Paweł; Woźniak, Magdalena; Asztemborska, Monika; Głód, Bronisław K.

doi:10.1007/s12161-013-9776-4

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…In addition, one of the quercetin derivatives has proven to have a protective effect on the intact β-cells of the islets of Langerhans by inhibiting lipid peroxidation, as well as its ability to scavenge free radicals from STZ-induced oxidative stress in STZ-induced diabetic rats [ 20 ]. Although no research has been conducted regarding the proficiency of 6-hydroxyflavanone on directly reducing the blood glucose level, there are some studies that have demonstrated the electron donating ability of 6-hydroxyflavanone to scavenge free radicals induced by 2,2-diphenyl-1-picrylhydrazyl in in vitro antioxidant study [ 21 , 59 ]. Besides that, kaempferol, another flavonoid-type compound that was identified and quantified, also reportedly shows hypoglycemic effect by lowering the levels of glycoprotein in the liver and increment of insulin production, as well as enhancement of glucose utilization in STZ-induced rats [ 22 ].…”

Section: Resultsmentioning

confidence: 99%

In Vitro Anti-Diabetic Activities and UHPLC-ESI-MS/MS Profile of Muntingia calabura Leaves Extract

et al. 2022

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Anti-diabetic compounds from natural sources are now being preferred to prevent or treat diabetes due to adverse effects of synthetic drugs. The decoction of Muntingia calabura leaves was traditionally consumed for diabetes treatment. However, there has not been any published data currently available on the processing effects on this plant’s biological activity and phytochemical profile. Therefore, this study aims to evaluate the effect of three drying methods (freeze-drying (FD), air-drying (AD), and oven-drying (OD)) and ethanol:water ratios (0, 50, and 100%) on in vitro anti-diabetic activities of M. calabura leaves. In addition, an ultrahigh-performance-liquid chromatography–electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method was used to characterize the metabolites in the active extract. The FD M. calabura leaves, extracted with 50% ethanol, is the most active extract that exhibits a high α-glucosidase and α-amylase inhibitory activities with IC50 values of 0.46 ± 0.05 and 26.39 ± 3.93 µg/mL, respectively. Sixty-one compounds were tentatively identified by using UHPLC-ESI-MS/MS from the most active extract. Quantitative analysis, by using UHPLC, revealed that geniposide, daidzein, quercitrin, 6-hydroxyflavanone, kaempferol, and formononetin were predominant compounds identified from the active extract. The results have laid down preliminary steps toward developing M. calabura leaves extract as a potential source of bioactive compounds for diabetic treatment.

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Section: Resultsmentioning

confidence: 99%

In Vitro Anti-Diabetic Activities and UHPLC-ESI-MS/MS Profile of Muntingia calabura Leaves Extract

et al. 2022

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“…Compared with UV and DAD, ECD can identify changes in the electrical signals of substances and selectively detect compounds with oxidizing or reducing abilities [24][25][26]. Indeed, electrochemistry is an effective means for evaluating the antioxidant activity of a species, wherein it has been documented that there is a significant correlation between the DPPH scavenging activity and the peak height or the first half-wave potential [27,28]. Phenolic acids and flavonoids tend to exhibit superior response values by HPLC-electrochemical detection (HPLC-ECD) than compounds that do not bear phenolic hydroxyl groups [26,29].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the phytochemicals and antioxidant activity of Lophatherum gracile Brongn based on chemical fingerprinting by HPLC with electrochemical detection

Zhang

Chun

Shao

et al. 2021

J of Separation Science

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A combinative method using high-performance liquid chromatographyelectrochemical detection for fingerprinting and quantitative analysis was developed and successfully applied for the quality evaluation of Lophatherum gracile Brongn leaves collected from 21 geographical locations in China. In the fingerprint analysis, 18 common peaks were observed among the 21 samples, and 10 peaks were identified. Simultaneous quantification of the 10 components was conducted to interpret the variations in these compounds among the L. gracile Brongn leaves originating from different geographical locations. The correlation between the chromatograms and the antioxidant activities of the samples was further studied. The results indicated a linear correlation between the antioxidant activity and the total common peak areas of the fingerprints obtained by high-performance liquid chromatographyelectrochemical detection. Importantly, it was found that high-performance liquid chromatography-electrochemical detection fingerprinting can not only determine the quantities of individual components present in such samples but also evaluate the antioxidant activities of the samples. The developed method is a valuable reference for the further study and development of L. gracile Brongn.

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“…Flavonoids are phenolic compounds with a chemical structure of C 6 -C 3 -C 6 . Flavonoids have the conjugated bonds, there is a single bonded (C-C) and double bonded (C = C) carbon atoms intermittent [16].…”

Section: Introductionmentioning

confidence: 99%

Electrical conductivity modification using silver nano particles of Jatropha Multifida L. and Pterocarpus Indicus w. extracts films

Diantoro¹,

Hidayati²,

Latifah³

et al. 2016

AIP Conference Proceedings

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Synthesis and characterization of highly purified nanosilica from pyrophyllite ores AIP Conference Proceedings 1719, 030020 (2016) A number of research have been reported related to broad synthesis method and mechanical properties. So far there is no specific report of electrical conductivity associated to PIW and JIL natural polymers. In order to obtain electrical conductivity and its crystallinity of the extracted polymer films, it was induced on them a various fraction of silver nano particles. The film has been prepared by means of spin coating method on nickel substrate. It was revealed that FTIR spectra confirm the existing of rutine flavonoid. The crystallinity of the samples increase from 0.66%, to 4.11% associated to the respective various of silver fractions of 0.1 M to 0.5 M. SEM images show that there are some grains of silver in the film. The nature of electric conductivity increases a long with the addition of silver. The electrical conductivity increase significantly from 3.22 S/cm, to 542.85 S/cm. On the other hand, PIW films also shows similar trends that increase of Ag induce the increase its crystallinity as well as its electrical conductivity at semiconducting level. This result opens a prospective research and application of the green renewable polymer as optoelectronic materials.

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Comparative Analysis of Antioxidative Activity of Flavonoids Using HPLC–ED and Photometric Assays

Cited by 6 publications

References 17 publications

In Vitro Anti-Diabetic Activities and UHPLC-ESI-MS/MS Profile of Muntingia calabura Leaves Extract

In Vitro Anti-Diabetic Activities and UHPLC-ESI-MS/MS Profile of Muntingia calabura Leaves Extract

Evaluation of the phytochemicals and antioxidant activity of Lophatherum gracile Brongn based on chemical fingerprinting by HPLC with electrochemical detection

Electrical conductivity modification using silver nano particles of Jatropha Multifida L. and Pterocarpus Indicus w. extracts films

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